Genetic Differentiation of Portuguese Tea Plant using RAPD Markers DOI Open Access

S. Jorge,

M. Cristina Pedroso,

David B. Neale

et al.

HortScience, Journal Year: 2003, Volume and Issue: 38(6), P. 1191 - 1197

Published: Oct. 1, 2003

Random amplified polymorphic DNA (RAPD) analysis was used to estimate genetic similarities between Portuguese Camelliasinensis (L.) O. Kuntze (tea plant) accessions and those obtained from the germplasm collections Tea Research Foundation of Kenya National Institute Vegetables, Ornamental Plants, Japan. The studied are taxonomically classified as C. sinensis , var. assamica or ssp. lasiocalyx . A set 118 ten-base arbitrary primers tested, which 25 produced informative, reproducible, banding patterns. These were amplify 71 tea plant a total 282 bands, 195 polymorphic. phenotypic frequencies calculated using Shannon's Index employed in estimating diversity within populations. Our study demonstrates that populations, including plants, show considerable variability. From UPGMA cluster based on matrix Jaccard coefficient, it possible distinguish plants remaining accessions. RAPD markers discriminated three varieties. Moreover, each variety cluster, subclusters formed according geographic distribution. also separated commercially cultivated Taiwanese wild plants. present results constitutes good method sinensis, differentiate taxonomic geographical

Language: Английский

Genetic Differentiation of Portuguese Tea Plant using RAPD Markers DOI Open Access

S. Jorge,

M. Cristina Pedroso,

David B. Neale

et al.

HortScience, Journal Year: 2003, Volume and Issue: 38(6), P. 1191 - 1197

Published: Oct. 1, 2003

Random amplified polymorphic DNA (RAPD) analysis was used to estimate genetic similarities between Portuguese Camelliasinensis (L.) O. Kuntze (tea plant) accessions and those obtained from the germplasm collections Tea Research Foundation of Kenya National Institute Vegetables, Ornamental Plants, Japan. The studied are taxonomically classified as C. sinensis , var. assamica or ssp. lasiocalyx . A set 118 ten-base arbitrary primers tested, which 25 produced informative, reproducible, banding patterns. These were amplify 71 tea plant a total 282 bands, 195 polymorphic. phenotypic frequencies calculated using Shannon's Index employed in estimating diversity within populations. Our study demonstrates that populations, including plants, show considerable variability. From UPGMA cluster based on matrix Jaccard coefficient, it possible distinguish plants remaining accessions. RAPD markers discriminated three varieties. Moreover, each variety cluster, subclusters formed according geographic distribution. also separated commercially cultivated Taiwanese wild plants. present results constitutes good method sinensis, differentiate taxonomic geographical

Language: Английский

Citations

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