Novel bacterial lineages assembled from wastewater-impacted river metagenomes unveil ecosystem functions and risk of antibiotic resistance spread in the community DOI
Ranadhir Chakraborty, Ayan Paul, Partha Barman

et al.

Published: April 28, 2025

Abstract The Mahananda River, coursing through Siliguri, India, is subject to intense anthropogenic stressors, including urban wastewater, industrial effluents, and agrochemical runoff, necessitating a high-resolution microbial assessment. Leveraging whole-metagenome sequencing, we analyzed midstream water samples (APMG02 APMG03) reconstructed 25 metagenome-assembled genomes (MAGs), of which 14 met stringent quality criteria. We identified four novel bacterial species, Candidatus Aquabacterium mahanandaense APR203, Sulfurospirillum siliguriense APR208, Fluviibacter nbuensis APR306, Paludibacter darjeelingensis APR314, exhibited critical genomic signatures associated with hydrocarbon degradation, heavy metal detoxification, quorum sensing, stress response, underscoring plasticity in response pollutant influx. Detecting pathogenicity-associated factors, antimicrobial resistance determinants, mobile genetic elements raise public health concerns. Comparative global river-water microbiome analyses delineate distinct anthropogenically sculpted consortium the emphasizing profound ecological ramifications unchecked contamination. This study underscores transformative potential metagenomics deciphering riverine dynamics resilience.

Language: Английский

Novel bacterial lineages assembled from wastewater-impacted river metagenomes unveil ecosystem functions and risk of antibiotic resistance spread in the community DOI
Ranadhir Chakraborty, Ayan Paul, Partha Barman

et al.

Published: April 28, 2025

Abstract The Mahananda River, coursing through Siliguri, India, is subject to intense anthropogenic stressors, including urban wastewater, industrial effluents, and agrochemical runoff, necessitating a high-resolution microbial assessment. Leveraging whole-metagenome sequencing, we analyzed midstream water samples (APMG02 APMG03) reconstructed 25 metagenome-assembled genomes (MAGs), of which 14 met stringent quality criteria. We identified four novel bacterial species, Candidatus Aquabacterium mahanandaense APR203, Sulfurospirillum siliguriense APR208, Fluviibacter nbuensis APR306, Paludibacter darjeelingensis APR314, exhibited critical genomic signatures associated with hydrocarbon degradation, heavy metal detoxification, quorum sensing, stress response, underscoring plasticity in response pollutant influx. Detecting pathogenicity-associated factors, antimicrobial resistance determinants, mobile genetic elements raise public health concerns. Comparative global river-water microbiome analyses delineate distinct anthropogenically sculpted consortium the emphasizing profound ecological ramifications unchecked contamination. This study underscores transformative potential metagenomics deciphering riverine dynamics resilience.

Language: Английский

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