In vitro assessment of Bionectria ochroleuca metabolites: A promising approach for controlling root-knot nematode, Meloidogyne incognita DOI Open Access

Vyamasani Shravani,

Saranya Nallusamy,

Jothi Govindasamy

et al.

Plant Science Today, Journal Year: 2024, Volume and Issue: 11(sp4)

Published: Dec. 29, 2024

Meloidogyne incognita, a highly destructive root-knot nematode, causes substantial crop losses worldwide by infesting plant roots, which disrupts nutrient and water uptake. This pest is notoriously challenging to manage due its broad host range growing resistance many chemical nematicides, emphasizing the urgent need for sustainable, eco-friendly alternatives. In this context, current study investigated nematicidal potential of secondary metabolites derived from entomopathogenic fungus, Bionectria ochroleuca against M. incognita. The in vitro assays demonstrated dose- time-dependent inhibition egg hatching juvenile survival. At crude metabolite concentration 100%, was reduced 5.64% mortality increased 95.4% after 72 h. Gas Chromatography-Mass Spectrometry (GC-MS) analysis identified key metabolites, including palmitic acid, butanedioic lactic oleic appear inhibit nematode growth through mechanisms that impair cell membrane integrity, disrupt energy metabolism, interfere with essential metabolic pathways. Further, enrichment revealed their involvement biosynthetic pathways, such as unsaturated fatty acids, galactose phenylalanine metabolism. Molecular docking studies supported these findings showing high binding affinities virulent proteins, Cytochrome c oxidase subunit 1 NAD(H) oxidase, suggesting interference biological processes within nematode. Overall, position B. promising candidates managing infestations, offering potent alternative nematicides thereby contributing sustainable agricultural practices.

Language: Английский

In vitro assessment of Bionectria ochroleuca metabolites: A promising approach for controlling root-knot nematode, Meloidogyne incognita DOI Open Access

Vyamasani Shravani,

Saranya Nallusamy,

Jothi Govindasamy

et al.

Plant Science Today, Journal Year: 2024, Volume and Issue: 11(sp4)

Published: Dec. 29, 2024

Meloidogyne incognita, a highly destructive root-knot nematode, causes substantial crop losses worldwide by infesting plant roots, which disrupts nutrient and water uptake. This pest is notoriously challenging to manage due its broad host range growing resistance many chemical nematicides, emphasizing the urgent need for sustainable, eco-friendly alternatives. In this context, current study investigated nematicidal potential of secondary metabolites derived from entomopathogenic fungus, Bionectria ochroleuca against M. incognita. The in vitro assays demonstrated dose- time-dependent inhibition egg hatching juvenile survival. At crude metabolite concentration 100%, was reduced 5.64% mortality increased 95.4% after 72 h. Gas Chromatography-Mass Spectrometry (GC-MS) analysis identified key metabolites, including palmitic acid, butanedioic lactic oleic appear inhibit nematode growth through mechanisms that impair cell membrane integrity, disrupt energy metabolism, interfere with essential metabolic pathways. Further, enrichment revealed their involvement biosynthetic pathways, such as unsaturated fatty acids, galactose phenylalanine metabolism. Molecular docking studies supported these findings showing high binding affinities virulent proteins, Cytochrome c oxidase subunit 1 NAD(H) oxidase, suggesting interference biological processes within nematode. Overall, position B. promising candidates managing infestations, offering potent alternative nematicides thereby contributing sustainable agricultural practices.

Language: Английский

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