Toll-like receptor4 and 8 are overexpressed in lung biopsies of human non-small cell lung carcinoma DOI Creative Commons
Silvia Ceccarelli,

Viola Pasqua Marzolesi,

Jacopo Vannucci

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 13, 2024

Abstract Purpose Lung cancer is the leading cause of death worldwide which includes two main types carcinoma distinguished in non-small cell lung (NSCLC) involving epithelial cells, and small (SCLC) affecting neuronal cells hormone secreting cells. Studies have shown a causal link between inflammation/innate immunity onset NSCLC. Hence, present study aimed to evaluate diagnostic prognostic value Toll-like receptors (TLRs), particular TLR4 TLR8 patients with NSCLC.Methods Patients surgically treated for NSCLC anatomical resections benign disease were enrolled. The expression levels determined by real time PCR immunohistochemical analysis PBMC tissues, respectively. A preliminary silico included 1194 arrays from healthy tissues extracted Genevestigator database. association TLRs gene survival outcome was also investigated.Results Bioinformatics showed downregulation positively impacts adenocarcinoma (LUAD) squamous (LUSC). However, no significant differences case control groups observed PBMC. positive correlation found their levels. Interestingly, that higher than group either as staining intensity cells.Conclusions Albeit precise role not fully defined, this identified pathogenesis Our data support possible increase overall may serve potential biomarker

Language: Английский

Toll-Like Receptor 4 and 8 are Overexpressed in Lung Biopsies of Human Non-small Cell Lung Carcinoma DOI Creative Commons
Silvia Ceccarelli,

Viola Pasqua Marzolesi,

Jacopo Vannucci

et al.

Lung, Journal Year: 2025, Volume and Issue: 203(1)

Published: March 1, 2025

Abstract Purpose Lung cancer is the leading cause of death worldwide which includes two main types carcinoma distinguished in non-small cell lung (NSCLC) involving epithelial cells, and small (SCLC) affecting neuronal cells hormone secreting cells. Studies have shown a causal link between inflammation/innate immunity onset NSCLC. The present study aimed to evaluate expression Toll-like receptors (TLRs) 4 TLR8 peripheral blood mononuclear (PBMC) tissues patients with NSCLC, useful for future prognostic tools Methods Patients surgically treated NSCLC anatomical resections benign disease were enrolled. levels TLR4 determined by real time PCR immunohistochemical analysis PBMC tissues, respectively. A preliminary silico including 1194 arrays from healthy extracted Genevestigator database. association TLRs gene survival outcome was also investigated. Results Bioinformatics revealed that downregulation positively impacts adenocarcinoma (LUAD) squamous (LUSC). However, no significant differences case control groups observed PBMC. positive correlation found their levels. Interestingly, showed higher than group terms staining intensity Conclusion Albeit precise role not fully defined, this identified potential involvement pathogenesis Our data led us hypothesize overall deserves be explored further establish whether can represent indicators

Language: Английский

Citations

0
Toll-like receptor4 and 8 are overexpressed in lung biopsies of human non-small cell lung carcinoma DOI Creative Commons
Silvia Ceccarelli,

Viola Pasqua Marzolesi,

Jacopo Vannucci

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 13, 2024

Abstract Purpose Lung cancer is the leading cause of death worldwide which includes two main types carcinoma distinguished in non-small cell lung (NSCLC) involving epithelial cells, and small (SCLC) affecting neuronal cells hormone secreting cells. Studies have shown a causal link between inflammation/innate immunity onset NSCLC. Hence, present study aimed to evaluate diagnostic prognostic value Toll-like receptors (TLRs), particular TLR4 TLR8 patients with NSCLC.Methods Patients surgically treated for NSCLC anatomical resections benign disease were enrolled. The expression levels determined by real time PCR immunohistochemical analysis PBMC tissues, respectively. A preliminary silico included 1194 arrays from healthy tissues extracted Genevestigator database. association TLRs gene survival outcome was also investigated.Results Bioinformatics showed downregulation positively impacts adenocarcinoma (LUAD) squamous (LUSC). However, no significant differences case control groups observed PBMC. positive correlation found their levels. Interestingly, that higher than group either as staining intensity cells.Conclusions Albeit precise role not fully defined, this identified pathogenesis Our data support possible increase overall may serve potential biomarker

Language: Английский

Citations

0