Recent advances of CRISPR-based genome editing for enhancing staple crops

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 23, 2024

An increasing population, climate change, and diminishing natural resources present severe threats to global food security, with traditional breeding genetic engineering methods often falling short in addressing these rapidly evolving challenges. CRISPR/Cas systems have emerged as revolutionary tools for precise modifications crops, offering significant advancements resilience, yield, nutritional value, particularly staple crops like rice maize. This review highlights the transformative potential of technology, emphasizing recent innovations such prime base editing, development novel CRISPR-associated proteins, which significantly improved specificity, efficiency, scope genome editing agriculture. These enable targeted that enhance tolerance abiotic stresses well biotic stresses. Additionally, plays a crucial role improving crop yield quality by enhancing photosynthetic nutrient uptake, resistance lodging, while also taste, texture, shelf life, content through biofortification. Despite challenges off-target effects, need more efficient delivery methods, ethical regulatory concerns, underscores importance security sustainability It calls continued research integration CRISPR other emerging technologies nanotechnology, synthetic biology, machine learning fully realize its developing resilient, productive, sustainable agricultural systems.

Language: Английский

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Antisense DNA acaricide targeting pre-rRNA of two-spotted spider mite Tetranychus urticae as efficacy-enhancing agent of fungus Metarhizium robertsii

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 29, 2024

Abstract Two-spotted spider mite Tetranychus urticae Koch (Acari: Tetranychidae) is one of the most dangerous pests in world and pesticide-resistant species ever. Complex biological preparations are great interest for acaricide market because they do not poison ecosystems bioaccumulate food products, simultaneously, more slowly develop resistance to complex preparations. In this study we applied bioformulation composed 11-mer antisense oligonucleotide (oligonucleotide or DNA acaricide) Tur-3 fungus Metarhizium robertsii T. control. We discovered that joint contact application M. significantly attenuates reproduction rate mite. Our results indicate act synergistically lead a significant 7-times elevated mortality 5-times reduced fecundity Oligonucleotide causes 2,5-fold re-duction expression target pre-rRNA suppresses activity key players detoxifying enzymes its defense system (phenoloxidase, esterase, glutathione-S-transferase), on average, by 2-3 times. interferes with protein biosynthesis causing decrease production pest. Obviously, attenuation enhances fungal infection or/and produces variety degrade destroy integument structure pest, aiding penetration Tur-3. This research article first evidence successful together efficient Combined acaricides based conservative sequences rDNA fungi potent selective approach eco-friendly plant protection.

Language: Английский

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A Comparative Analysis of Immune Response, Gut Microbiota, and Susceptibility to Bacillus thuringiensis Bacteria in the Colorado Potato Beetle Leptinotarsa decemlineata

Journal of Evolutionary Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 60(6), P. 2326 - 2342

Published: Nov. 1, 2024

Language: Английский

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Comparative anlysis of immune response, intestinal microbiota and susceptibility to <i>Bacillus Thuringiensis</i> bacteria of the colorado potato beetle <i>Leptinotarsa Decemlineata</i>

Журнал эволюционной биохимии и физиологии, Journal Year: 2024, Volume and Issue: 60(6)

Published: Nov. 15, 2024

Language: Английский

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