A brief history of insect neuropeptide and peptide hormone research DOI Creative Commons
Dick R. Nässel

Cell and Tissue Research, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Abstract This review briefly summarizes 50 years of research on insect neuropeptide and peptide hormone (collectively abbreviated NPH) signaling, starting with the sequencing proctolin in 1975. The first 25 years, before Drosophila genome, were characterized by efforts to identify novel NPHs biochemical means, mapping their distribution neurons, neurosecretory cells, endocrine cells intestine. Functional studies predominantly dealing hormonal aspects peptides many employed ex vivo assays. With annotation more specifically receptors other insects, a new era followed. started matching NPH ligands orphan receptors, localize improved detection methods. Important advances made introduction rich repertoire innovative molecular genetic approaches interfere expression or function receptors. These methods enabled cell- circuit-specific interference signaling for assays determine roles behavior physiology, imaging neuronal activity, analysis connectivity peptidergic circuits. Recent have seen dramatic increase reports multiple functions development, physiology behavior. Importantly, we can now appreciate pleiotropic NPHs, as well functional “networks” where state dependent ensures behavioral plasticity systemic homeostasis.

Language: Английский

Synaptic connectome of a neurosecretory network in the Drosophila brain DOI Open Access
Theresa H. McKim, Jayati Gera,

Ariana Julia B. Gayban

et al.

Published: Nov. 14, 2024

Hormones mediate inter-organ signaling which is crucial in orchestrating diverse behaviors and physiological processes including sleep activity, feeding, growth, metabolism reproduction. The pars intercerebralis lateralis insects represent major hubs contain neurosecretory cells (NSC) that produce various hormones. To obtain insight into how hormonal regulated, we have characterized the synaptic connectome of NSC adult Drosophila brain. Identification neurons providing inputs to multiple subtypes implicates diuretic hormone 44-expressing as a coordinator physiology behavior. Surprisingly, despite most having dendrites subesophageal zone (primary taste processing center), gustatory are largely indirect. We also deciphered pathways via olfactory relayed NSC. Further, our analyses revealed substantial from descending NSC, suggesting regulate both endocrine motor output synchronize changes with appropriate behaviors. In contrast inputs, sparse mostly mediated by corazonin Therefore, additionally determine putative paracrine interconnectivity between peripheral tissues analyzing single-cell transcriptomic datasets. Our comprehensive characterization network provides platform understand complex networks they orchestrate animal physiology.

Language: Английский

Citations

2
Discovery of Paralogous GnRH and Corazonin Signaling Systems in an Invertebrate Chordate DOI Creative Commons
Luis Alfonso Yañez Guerra, Meet Zandawala

Genome Biology and Evolution, Journal Year: 2023, Volume and Issue: 15(7)

Published: June 9, 2023

Abstract Gonadotropin-releasing hormone (GnRH) is a key regulator of reproductive function in vertebrates. GnRH related to the corazonin (CRZ) neuropeptide which influences metabolism and stress responses insects. Recent evidence suggests that CRZ are paralogous arose by gene duplication common ancestor bilaterians. Here, we report identification complete characterization signaling systems amphioxus Branchiostoma floridae. We have identified novel peptide (YSYSYGFAP-NH2) specifically activates two receptors (FTYTHTW-NH2) three B. The latter appear be promiscuous, as can also activated physiological range. Hence, there potential for cross-talk between these closely systems. Discovery both one closest living relatives vertebrates provides framework discover their roles at transition from invertebrates

Language: Английский

Citations

5
The Neuropeptide Sulfakinin, a peripheral regulator of insect behavioral switch between mating and foraging DOI Creative Commons
Hongfei Li,

Bao Dong,

Yuanyuan Peng

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 31, 2024

Abstract Behavioral strategies for foraging and reproduction in the oriental fruit fly ( Bactrocera dorsalis ) are alternative options resource allocation controlled by neuropeptides. Here we show that behavioral switch between is associated with changes antennal sensitivity. Starved flies became more sensitive to food odors while suppressing their response opposite-sex pheromones. The gene encoding sulfakinin receptor 1 SkR1 was significantly upregulated antennae of starved flies, so tested phenotypes null mutants genes skr1 −/− its ligand sk ). In both knockout lines, responses shifted mating mode even when were starved. This suggests signaling via promotes mating. Further analysis mutant revealed sets odorant (OR) differentially expressed. Functional characterization expressed ORs suggested directly suppresses expression respond hormones enhancing detect volatiles. We conclude SkR1, modulating OR expressions leading altered antenna sensitivities, an important component starvation-dependent change.

Language: Английский

Citations

1
The Neuropeptide Sulfakinin, a peripheral regulator of insect behavioral switch between mating and foraging DOI Open Access
Hongfei Li,

Bao Dong,

Yuanyuan Peng

et al.

Published: Nov. 1, 2024

Behavioral strategies for foraging and reproduction in the oriental fruit fly ( Bactrocera dorsalis ) are alternative options resource allocation controlled by neuropeptides. Here we show that behavioral switch between is associated with changes antennal sensitivity. Starved flies became more sensitive to food odors while suppressing their response opposite-sex pheromones. The gene encoding sulfakinin receptor 1 SkR1 was significantly upregulated antennae of starved flies, so tested phenotypes null mutants genes skr1 −/− its ligand sk ). In both knockout lines, responses shifted mating mode even when were starved. This suggests signaling via promotes mating. Further analysis mutant revealed sets odorant (OR) differentially expressed. Functional characterization expressed ORs suggested directly suppresses expression respond hormones enhancing detect volatiles. We conclude SkR1, modulating OR expressions leading altered antenna sensitivities, an important component starvation-dependent change.

Language: Английский

Citations

1
A brief history of insect neuropeptide and peptide hormone research DOI Creative Commons
Dick R. Nässel

Cell and Tissue Research, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Abstract This review briefly summarizes 50 years of research on insect neuropeptide and peptide hormone (collectively abbreviated NPH) signaling, starting with the sequencing proctolin in 1975. The first 25 years, before Drosophila genome, were characterized by efforts to identify novel NPHs biochemical means, mapping their distribution neurons, neurosecretory cells, endocrine cells intestine. Functional studies predominantly dealing hormonal aspects peptides many employed ex vivo assays. With annotation more specifically receptors other insects, a new era followed. started matching NPH ligands orphan receptors, localize improved detection methods. Important advances made introduction rich repertoire innovative molecular genetic approaches interfere expression or function receptors. These methods enabled cell- circuit-specific interference signaling for assays determine roles behavior physiology, imaging neuronal activity, analysis connectivity peptidergic circuits. Recent have seen dramatic increase reports multiple functions development, physiology behavior. Importantly, we can now appreciate pleiotropic NPHs, as well functional “networks” where state dependent ensures behavioral plasticity systemic homeostasis.

Language: Английский

Citations

1