Multi‐omics revealed that DCP1A and SPDL1 determine embryogenesis defects in postovulatory ageing oocytes DOI Creative Commons
Li Kong,

Yutian Gong,

Yongyong Wang

et al.

Cell Proliferation, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 4, 2024

Abstract Growing evidence indicates that the deterioration of egg quality caused by postovulatory ageing significantly hampers embryonic development. However, molecular mechanisms which leads to a decline in oocyte have not been fully characterized. In this study, we observed an accelerated decay maternal mRNAs through RNA‐seq analyses postovulatory‐aged (PostOA) oocytes. We noted these downregulated should be degraded during 2‐cell stage. Proteomic revealed degradation is associated with accumulation DCP1A. The injection exogenous Dcp1a mRNA or siRNA into MII stage oocytes proved DCP1A could accelerate mRNAs. Additionally, also found SPDL1 crucial for maintaining spindle/chromosome structure and chromosome euploidy PostOA Spdl1‐ remarkably recovered meiotic defects Collectively, our findings provide valuable insights underlying ageing.

Language: Английский

A Single‐Cell Transcriptome Atlas Characterizes the Immune Landscape of Human Testes During Aging DOI Creative Commons

Qiaoling Jiang,

Lina Cui,

Xichen Nie

et al.

Aging Cell, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

Aging disrupts immune regulation, affecting tissue function and increasing vulnerability to various diseases. However, the effects of aging on cells within human testes are not well understood. In this study, we utilized single-cell RNA sequencing profile from 33 testis samples individuals aged 21 69. Our analysis revealed key cell types, including CD8+ T cells, monocytes, cDC2 macrophage subtypes testes. We observed an age-related change in monocytes MRC1hi tissue-resident (TRM), a pattern consistent both mouse Individuals 40 older showed notable shifts pathways related phagocytosis, cytokine signaling, antigen presentation. Monocytes also exhibited pro-inflammatory characteristics, potentially contributing low-grade inflammation commonly associated with aging. Together, these findings provide insights into alterations uncover molecular mechanisms underlying shifts, offering valuable resource for understanding reproductive system.

Language: Английский

Citations

0
Algal and Fungal Antioxidants Alleviate Oxidative Stress‐Induced Reproductive Defects DOI Creative Commons
Nazli Pinar Arslan, Seyda Albayrak,

Aysenur Budak‐Savas

et al.

Food Science & Nutrition, Journal Year: 2025, Volume and Issue: 13(5)

Published: May 1, 2025

The intrinsic and extrinsic factors (natural aging, diseases, infections, chemicals, radiation, heavy metals etc.) create oxidative stress, thereby causing reproductive defects in males females. Similarly, stress causes the abnormalities sperm oocytes, thus reduces success of vitro fertilization. Fungi and/or algae-derived metabolites (polysaccharides, carotenoids, ergothioneine, phycobiliproteins, phlorotannins, cordycepin) alleviate damages ovaries testis correct impaired functions (spermatogenesis, ovulation, fertilization implantation) vivo models by increasing antioxidant enzyme activities (superoxide dismutase, catalase, glutathione peroxidase etc.), making mitochondrial parameters better (membrane potential, distribution, dynamics activity mitochondria), decreasing (the reductions intracellular ROS malondialdehyde levels), inflammation COX-2, IL-6, IL-1β, TNF-α levels) apoptosis Caspase-3 Bax balancing blood hormone levels (follicle stimulating hormone, luteinizing testosterone). models, they increase enzymes activities, decrease apoptosis, make better, restoring oocyte blastocyst formation. Overall, this review study reveals that antioxidants from fungi algae contribute to improvement outcome humans animals assisted technology preventing or alleviating stress. However, more vitro, vivo, clinical studies are needed prove safety profile efficacy fungi- algae-based antioxidants. This is first focusing on protective effects against stress-induced defects.

Language: Английский

Citations

0
Identification of key genes associated with infertile endometriosis based on bioinformatic analysis DOI Creative Commons
Xiaocong Chen, Hongjuan Ye, Xin Xie

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 18, 2024

Abstract Background: Endometriosis is a common disease among women of childbearing age. However, the molecular mechanism behind it still unknown. Therefore, new biomarkers and therapeutic targets are needed to improve diagnosis treatment infertile women. Methods: Microarray datasets GSE7305, GSE7307, GSE51981 were downloaded from Gene Expression Omnibus (GEO) database identify differentially expressed genes (DEGs) between control endometriosis. The STRING Cytoscape software constructed protein-protein interaction (PPI) hub gene networks. At same time, three data sets screened for co-differentially related mitosis. Subsequently, we identified mitosis-related (MRHGs) associated with both genes. Next, enrichment analysis target was performed by Ontology (GO) annotation Kyoto Encyclopedia Genes Genomes (KEGG) pathway, mRNA-miRNA network constructed. Finally, GSE25628 GSE6364 used verify expression MRHGs individually, while GSE120103 employed ascertain influence on female fertility. Results: A total 93 DEGs in endometriosis datasets. Then, placed 11 potential downregulated genes, which eight showed good diagnostic properties endometriosis, two main enriched GO functions revealed that cell cycle mitotic pathway may be critical Meanwhile, networks choosing co-expressed mRNAs miRNAs. Furthermore, cordycepin high drug-targeting relevance Conclusions: We as diagnosing treating CENPE CCNA2 might affecting endometrial secretory phase transition. In addition, clinical people infertility-related

Language: Английский

Citations

0
Cordycepin Ameliorates Spaceflight-Induced Osteoporosis by Preventing BMSCs Oxidative Stress and Senescence via Interacting with PI3K p110α and Regulating PI3K/Akt/FOXO3 Signalling DOI Creative Commons

Yu Zhai,

Yuyao Zhang, Kexin Xu

et al.

Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

Citations

0
Diosmetin Promotes Early Embryonic Development in Pigs by Alleviating Oxidative Stress DOI

Jia‐Jun Ren,

Xiu‐Wen Yuan,

Zilong Meng

et al.

Molecular Reproduction and Development, Journal Year: 2024, Volume and Issue: 91(9)

Published: Sept. 1, 2024

ABSTRACT Diosmetin (DIOS), a natural flavonoid monomer derived from lemons and present in various plants such as spearmint spider moss, exhibits antioxidant, anti‐inflammatory, antiaging properties. Nonetheless, its impact on early embryonic development pigs remains unexplored. This study aimed to determine the influence of DIOS supplementation an vitro culture (IVC) medium porcine embryo elucidate underlying mechanisms. Findings revealed that embryos cultured IVC with 0.1 μM demonstrated increased blastocyst formation rate, higher total cell number, reduced LC3B CASPASE3 levels, elevated Nrf2 decreased ROS, enhanced GSH mitochondrial membrane potential at 4‐cell stage. Additionally, expression proapoptotic genes ( CAS3 , CAS8 BAX ) autophagy‐related BECLIN1 ATG5 P62 was downregulated, whereas development‐related CDK1 CDK2 ), antioxidant‐related SOD1 SOD2 biogenesis‐related NRF2 upregulated. These findings suggest promotes by mitigating oxidative stress enhancing function, thereby reducing autophagy apoptosis levels.

Language: Английский

Citations

0
Single-cell multi-omics analysis of in vitro post-ovulatory aged oocytes revealed aging-dependent protein degradation DOI Creative Commons
Yueshuai Guo,

Mengmeng Gao,

Xiaofei Liu

et al.

Molecular & Cellular Proteomics, Journal Year: 2024, Volume and Issue: unknown, P. 100882 - 100882

Published: Nov. 1, 2024

Language: Английский

Citations

0
Multi‐omics revealed that DCP1A and SPDL1 determine embryogenesis defects in postovulatory ageing oocytes DOI Creative Commons
Li Kong,

Yutian Gong,

Yongyong Wang

et al.

Cell Proliferation, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 4, 2024

Abstract Growing evidence indicates that the deterioration of egg quality caused by postovulatory ageing significantly hampers embryonic development. However, molecular mechanisms which leads to a decline in oocyte have not been fully characterized. In this study, we observed an accelerated decay maternal mRNAs through RNA‐seq analyses postovulatory‐aged (PostOA) oocytes. We noted these downregulated should be degraded during 2‐cell stage. Proteomic revealed degradation is associated with accumulation DCP1A. The injection exogenous Dcp1a mRNA or siRNA into MII stage oocytes proved DCP1A could accelerate mRNAs. Additionally, also found SPDL1 crucial for maintaining spindle/chromosome structure and chromosome euploidy PostOA Spdl1‐ remarkably recovered meiotic defects Collectively, our findings provide valuable insights underlying ageing.

Language: Английский

Citations

0