The role and mechanism of NAT10‐mediated ac4C modification in tumor development and progression DOI Creative Commons

Zhuoran Gu,

Libin Zou,

Xinjian Pan

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(12)

Published: Dec. 1, 2024

Abstract RNA modification has emerged as a crucial area of research in epigenetics, significantly influencing tumor biology by regulating metabolism. N‐acetyltransferase 10 (NAT10)‐mediated N4‐acetylcytidine (ac4C) modification, the sole known acetylation eukaryotic RNA, influences cancer pathogenesis and progression. NAT10 is only writer ac4C catalyzes acetyl transfer on targeted helps to improve stability translational efficiency ac4C‐modified RNA. highly expressed associated with poor prognosis pan‐cancers. Based its molecular mechanism biological functions, central factor tumorigenesis, progression, drug resistance, immune escape. Despite increasing focus ac4C, specific regulatory mechanisms remain elusive. The present review thoroughly analyzes current knowledge NAT10‐mediated cancer, highlighting broad influence gene expression biology. This also summarizes limitations perspectives identify new therapeutic targets advance treatment strategies.

Language: Английский

Synthesis of Sensitive Oligodeoxynucleotides Containing Acylated Cytosine, Adenine, and Guanine Nucleobases DOI Creative Commons

Komal Chillar,

Rohith Awasthy,

Marina Tanasova

et al.

DNA, Journal Year: 2025, Volume and Issue: 5(2), P. 25 - 25

Published: May 9, 2025

Background/Objective: Oligodeoxynucleotides (ODNs) containing base-labile modifications such as N4-acetyldeoxycytidine (4acC), N6-acetyladenosine (6acA), N2-acetylguanosine (2acG), and N4-methyoxycarbonyldeoxycytidine (4mcC) are highly challenging to synthesize because standard ODN synthesis methods require deprotection cleavage under strongly basic nucleophilic conditions, there is a lack of ideal alternative solve the problem. The objective this work explore capability recently developed 1,3-dithian-2-yl-methoxycarbonyl (Dmoc) method for incorporation multiple 4acC into single molecule feasibility using 6acA, 2acG 4mcC ODNs. Methods: sensitive ODNs were synthesized on an automated solid phase synthesizer Dmoc group linker methyl (meDmoc) protection exo-amino groups nucleobases. Deprotection achieved non-nucleophilic weakly conditions. Results: 4acC, 2acG, all found be stable mild Up four able incorporated 19-mer molecule. also successfully synthesized. characterized RP HPLC, capillary electrophoresis, gel electrophoresis MALDI MS. Conclusions: Among modified nucleotides, has been in nature proven beneficial DNA duplex stability. A expected find applications biological studies involving 4acC. Although have not nature, synthetic route them facilitate projects aimed at studying their biophysical properties well potential antisense, RNAi, CRISPR, mRNA therapeutic applications.

Language: Английский

Citations

0
Acetylcytidine modification of Amotl1 by N-acetyltransferase 10 contributes to cardiac fibrotic expansion in mice after myocardial infarction DOI
Xiuxiu Wang, Yiming Zhao,

Qian-yun Zhang

et al.

Acta Pharmacologica Sinica, Journal Year: 2024, Volume and Issue: 45(7), P. 1425 - 1437

Published: June 5, 2024

Language: Английский

Citations

2
RNA ac4C modification in cancer: Unraveling multifaceted roles and promising therapeutic horizons DOI
Wenhao Ouyang, Zhenjun Huang,

Keyu Wan

et al.

Cancer Letters, Journal Year: 2024, Volume and Issue: 601, P. 217159 - 217159

Published: Aug. 14, 2024

Language: Английский

Citations

2
A review of current developments in RNA modifications in lung cancer DOI Creative Commons
Shujun Zhang, Yafeng Liu, Kaijie Liu

et al.

Cancer Cell International, Journal Year: 2024, Volume and Issue: 24(1)

Published: Oct. 25, 2024

Lung cancer has the highest incidence and mortality rates worldwide is primary cause of cancer-related death. Despite rapid development diagnostic methods targeted drugs in recent years, many lung patients do not benefit from effective therapies. The emergence drug resistance led to a reduction therapeutic effectiveness drugs, highlighting crucial need explore novel targets. Many studies have found that epigenetic plays an important role occurrence cancer. This review describes biological function RNA modifications, such as m6A, m5C, m7G, m1A, advancements their development, progression, prognosis aims provide new guidance for treatment

Language: Английский

Citations

2
The role and mechanism of NAT10‐mediated ac4C modification in tumor development and progression DOI Creative Commons

Zhuoran Gu,

Libin Zou,

Xinjian Pan

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(12)

Published: Dec. 1, 2024

Abstract RNA modification has emerged as a crucial area of research in epigenetics, significantly influencing tumor biology by regulating metabolism. N‐acetyltransferase 10 (NAT10)‐mediated N4‐acetylcytidine (ac4C) modification, the sole known acetylation eukaryotic RNA, influences cancer pathogenesis and progression. NAT10 is only writer ac4C catalyzes acetyl transfer on targeted helps to improve stability translational efficiency ac4C‐modified RNA. highly expressed associated with poor prognosis pan‐cancers. Based its molecular mechanism biological functions, central factor tumorigenesis, progression, drug resistance, immune escape. Despite increasing focus ac4C, specific regulatory mechanisms remain elusive. The present review thoroughly analyzes current knowledge NAT10‐mediated cancer, highlighting broad influence gene expression biology. This also summarizes limitations perspectives identify new therapeutic targets advance treatment strategies.

Language: Английский

Citations

2