Enhanced itaconic acid secretion from macrophages mediates the protection of mesenchymal stem cell-derived exosomes on lipopolysaccharide-induced acute lung injury mice DOI Creative Commons

Yanmei Wen,

Zongan Liang

Biology Direct, Journal Year: 2024, Volume and Issue: 19(1)

Published: Dec. 26, 2024

Alveolar macrophages (AMs) is critical to exacerbate acute lung injury (ALI) induced by lipopolysaccharide (LPS) via inhibiting inflammation, which could shifted mesenchymal stem cell-derived exosomes (MSC-exos). But the underlying rationale not fully clarified. Our study aimed analyze significance of itaconic acid (ITA) in mediating protective effects MSC-exos on LPS-induced ALI. were used treat pulmonary microvascular endothelial cells (PMVECs) co-cultured with AMs under LPS stimulation. si-IRG1 was transfected AMs. PMVEC permeability, apoptosis rates, and inflammatory cytokine levels assessed. In vivo, C57BL/6 wild-type (WT) Irg1−/− mice employed explore protection against The determined histological biochemical assays. ITA measured using gas chromatography-mass spectrometry. Western blot flow cytometry analyses performed assess M1/M2 polarization. Co-culture significantly increased IL-6, TNF-α Claudin-5 ZO-1 expression treatment, attenuated accompanied enhanced level. After transfection, MSC-exos' efficacy reversed, suppressed M2 alleviated alveolar structure disruption, edema, inflammation concentration WT but had reduced mice, neglected secretion facilitated benefits damage ALI promoting AM polarization, highlighting a potential therapeutic strategy for related diseases.

Language: Английский

Application of lipopolysaccharide in establishing inflammatory models DOI
Xiao Zhang, Xiao Tian, Yan Wang

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 279, P. 135371 - 135371

Published: Sept. 6, 2024

Language: Английский

Citations

11
Organoids: development and applications in disease models, drug discovery, precision medicine, and regenerative medicine DOI Creative Commons
Qigu Yao, Sheng Cheng, Qiaoling Pan

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(10)

Published: Sept. 21, 2024

Organoids are miniature, highly accurate representations of organs that capture the structure and unique functions specific organs. Although field organoids has experienced exponential growth, driven by advances in artificial intelligence, gene editing, bioinstrumentation, a comprehensive overview organoid applications remains necessary. This review offers detailed exploration historical origins characteristics various types, their applications-including disease modeling, drug toxicity efficacy assessments, precision medicine, regenerative medicine-as well as current challenges future directions research. have proven instrumental elucidating genetic cell fate hereditary diseases, infectious metabolic disorders, malignancies, study processes such embryonic development, molecular mechanisms, host-microbe interactions. Furthermore, integration technology with intelligence microfluidics significantly advanced large-scale, rapid, cost-effective thereby propelling progress medicine. Finally, advent high-performance materials, three-dimensional printing technology, also gaining prominence Our insights predictions aim to provide valuable guidance researchers support continued advancement this rapidly developing field.

Language: Английский

Citations

6
Chinese Medicine Combined with Adipose Tissue-Derived Mesenchymal Stem Cells: A New Promising Aspect of Integrative Medicine DOI
Nan Nan, Na Yang, Liu Yang

et al.

Chinese Journal of Integrative Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Language: Английский

Citations

0
Enhancing Lung Recovery: Inhaled Poly(lactic-co-glycolic) Acid Encapsulating FTY720 and Nobiletin for Lipopolysaccharide-Induced Lung Injury, with Advanced Inhalation Tower Technology DOI Creative Commons
H Zhang, Wen‐Shuo Kuo,

Pei-Yun Tu

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 18, 2025

Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS), a rapidly progressing failure condition, results in high mortality rate, especially severe cases. Numerous trials have investigated various pharmacotherapy approaches, but their effectiveness remains uncertain. Here, we present an inhaled nanoformulation of fingolimod (FTY720)-nobiletin (NOB)- poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) with good biocompatibility and sustained-release pharmacological effect. The formulation decreases the toxicity FTY720 increases bioavailability NOB since use PLGA to encapsulate at same time. In vitro, comparison treatment pure drug, demonstrated that FTY720-NOB-PLGA NPs can reduce interleukin-6 (IL-6) reactive oxygen species (ROS) release by macrophages after lipopolysaccharide (LPS) stimulation more efficiently. vivo, used inhalation tower system allowed exposure unanesthetized mice aerosolized under controlled conditions. We attenuate LPS suppressing cytokine release, such as IL-6 tumor necrosis factor-α (TNF-α). trigger pathway ALI, including nuclear factor κ-light-chain-enhancer activated B cells (NF-κB) p38 mitogen-activated protein kinase, was also efficiently inhibited. Furthermore, provided safety profile, without detrimental effects on biochemical markers function. feasibility administering noninvasively continuous monitoring developed show excellent promise for acute therapy future.

Language: Английский

Citations

0
Co-culture of mesenchymal stem cells and macrophage: a narrative review DOI
Jun Ning, Rajiv Kumar Sah, Jing Wang

et al.

Journal of Pharmacology and Experimental Therapeutics, Journal Year: 2025, Volume and Issue: 392(4), P. 103531 - 103531

Published: March 5, 2025

Stem cell transplantation is a promising treatment for repairing damaged tissues, but challenges like immune rejection and ethical concerns remain. Mesenchymal stem cells (MSCs) offer high differentiation potential regulatory activity, showing promise in treating diseases such as gynecological, neurological, kidney disorders. With scientific progress, MSC applications are overcoming traditional limitations. In MSCs-macrophage coculture, MSCs transform macrophages into anti-inflammatory M2 macrophages, reducing inflammation, whereas enhance osteogenic differentiation. This coculture vital modulation tissue repair, with models varying by contact type dimensional arrangements. Factors techniques ratios influence outcomes. Benefits include improved heart function, wound healing, reduced lung accelerated bone repair. Challenges optimizing conditions. study reviews the methodologies, factors, mechanisms of MSC-macrophage providing foundation engineering applications. SIGNIFICANCE STATEMENT: review underlines significant role mesenchymal cell-macrophage application.

Language: Английский

Citations

0
The impact of the inflammatory pulmonary microenvironment on the behavior and function of mesenchymal stromal cells DOI

Abigail Warren,

Lanzhi Liu,

Daniel O’Toole

et al.

Expert Review of Respiratory Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: April 13, 2025

Acute respiratory distress syndrome is characterized by the dysregulation and activation of several inflammatory pathways which leads to widespread inflammation in lungs. Presently, direct therapy unavailable use mesenchymal stromal cells as a has been proposed, early phase studies have shown promise. MSCs exert various therapeutic effects on microenvironment, such anti-microbial effects, restoration alveolar-capillary barrier, exuding anti-inflammatory effects. However, perform these need be submitted specific external stimuli can affect their immunomodulation, survival, migration metabolic state. This review references articles found through targeted searches PubMed [Accessed between November 2024 March 2025], for key terms 'mesenchymal cells,' 'inflammatory microenvironment,' anti-inflammatory,' 'metabolism,' 'immunomodulation.' The advancement treatment ARDS not progressed effectively one might anticipated. Several clinical findings established patient subgroups based cytokine profiles severity ARDS. variation patients may influence efficacy instead concluding that worth pursuing, more research needed develop an appropriate therapy.

Language: Английский

Citations

0
Boling Huazhuo Pill-medicated serum alleviates inflammation of RWPE-1 cells by regulating M1 macrophage-derived CCL3 DOI

Yuping Peng,

Lu Liu

European Journal of Integrative Medicine, Journal Year: 2025, Volume and Issue: unknown, P. 102482 - 102482

Published: April 1, 2025

Language: Английский

Citations

0
Organoids in disease modeling and regenerative medicine DOI Creative Commons
Ryuji Morizane, Mart M. Lamers

Cellular and Molecular Life Sciences, Journal Year: 2025, Volume and Issue: 82(1)

Published: April 21, 2025

Organoid technology has the potential to revolutionize biomedical research by providing more physiologically relevant models for studying human development, disease mechanisms, and therapeutic development. Derived from stem cells, organoids self-organize into three-dimensional tissues that replicate structures functions of their in vivo counterparts. Their ability mimic organ-specific microstructures offers new tools investigating organogenesis, modeling genetic disorders, screening therapeutics using cells. Additionally, hold promise regenerative medicine as transplantable repairing or replacing damaged organs. However, challenges such batch variability, standardization, vascularization, long-term viability, lack immune cells remain, hindering clinical translation use studies. Recent efforts have focused on improving reproducibility, incorporating bioengineering techniques enhanced maturation, optimizing differentiation methods. This collection highlights recent advances respiratory, renal, retinal organoid systems. From refining cryopreservation methods virus neutralization inflammatory studies, these contributions emphasize translational medicine.

Language: Английский

Citations

0
Baicalin inhibits LPS-induced apoptosis and inflammation in WI- 38 cells by promoting FOXA2/TRIM27 Interaction: Implications for pediatric pneumonia mechanisms DOI
Lihua Gao, Xianyi Zeng, Yubo Huang

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Language: Английский

Citations

0
In vitro co-culture models for studying organoids-macrophages interaction: the golden technology of cancer immunotherapy DOI
Jinming Liu, Biao Zhang, Yuying Cui

et al.

American Journal of Cancer Research, Journal Year: 2024, Volume and Issue: 14(7), P. 3222 - 3240

Published: Jan. 1, 2024

Macrophages, as the largest immune cell group in tumour tissues, play a crucial role influencing various malignant behaviours of cells and evasion. As research on macrophages cancer immunotherapy develops, importance appropriate models becomes increasingly evident. The development organoids has bridged gap between traditional two-dimensional (2D) cultures animal experiments. Recent studies have demonstrated that exhibit similar physiological characteristics to source tissue closely resemble vivo genome molecular markers or organ. However, still lack an component. Developing co-culture model is for studying interaction mechanisms macrophages. This paper presents overview establishment models, current status organoid macrophage interactions, immunotherapy. In addition, application prospects shortcomings are explained. Ultimately, it hoped will offer preclinical testing platform maximising precise strategy.

Language: Английский

Citations

1