Biology Direct, Journal Year: 2024, Volume and Issue: 19(1)
Published: Dec. 26, 2024
Alveolar macrophages (AMs) is critical to exacerbate acute lung injury (ALI) induced by lipopolysaccharide (LPS) via inhibiting inflammation, which could shifted mesenchymal stem cell-derived exosomes (MSC-exos). But the underlying rationale not fully clarified. Our study aimed analyze significance of itaconic acid (ITA) in mediating protective effects MSC-exos on LPS-induced ALI. were used treat pulmonary microvascular endothelial cells (PMVECs) co-cultured with AMs under LPS stimulation. si-IRG1 was transfected AMs. PMVEC permeability, apoptosis rates, and inflammatory cytokine levels assessed. In vivo, C57BL/6 wild-type (WT) Irg1−/− mice employed explore protection against The determined histological biochemical assays. ITA measured using gas chromatography-mass spectrometry. Western blot flow cytometry analyses performed assess M1/M2 polarization. Co-culture significantly increased IL-6, TNF-α Claudin-5 ZO-1 expression treatment, attenuated accompanied enhanced level. After transfection, MSC-exos' efficacy reversed, suppressed M2 alleviated alveolar structure disruption, edema, inflammation concentration WT but had reduced mice, neglected secretion facilitated benefits damage ALI promoting AM polarization, highlighting a potential therapeutic strategy for related diseases.
Language: Английский