Phylogenetic analysis, metabolic profiling, and environmental adaptation of strain LCG007: a novel Rhodobacteraceae isolated from the East China Sea intertidal zone

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 7, 2025

Strain LCG007, isolated from Lu Chao Harbor's intertidal water, phylogenetically represents a novel genus within the family Rhodobacteraceae. Metabolically, it possesses wide array of amino acid metabolic genes that enable to thrive on both acids or peptides. Also, could hydrolyze peptides containing D-amino acids, highlighting its potential role in cycling refractory organic matter. Moreover, strain LCG007 utilize various carbohydrates, including mannopine and D-apiose-compounds primarily derived terrestrial plants-demonstrating capacity degrade It assimilate ammonia, nitrate nitrite, utilizes nitrogen sources such as polyamines, along with diverse inorganic phosphorus sulfur sources. Importantly, unlike very limited Sulfitobacter species possess photosynthetic genes, genomes LCG007-affiliated all Roseobacter harbor gene clusters. This conservation was further supported by significant impact light growth cell aggregation suggesting acquirement play crucial speciation their common ancestor. In terms environmental adaptability, encode for DNA photolyase, heat cold shock proteins, enzymes responsible scavenging reactive oxygen species, those involved uptake biosynthesis osmoprotectants betaine, γ-aminobutyric (GABA), trehalose collectively survive dynamic complex zone environment. Besides, biofilm formation is survival under conditions oligotrophy high salinity. study enhances our comprehension microbial taxonomy clade affiliated cluster, strategies ecosystems, underscores significance nutrient cycling. also highlights importance metabolism marine bacteria ecological resilience.

Language: Английский

The kinase LRRK2 is required for the physiological function and expression of the glial glutamate transporter EAAT2 (SLC1A2)

Journal of Neurochemistry, Journal Year: 2024, Volume and Issue: 169(1)

Published: Dec. 10, 2024

Neurotransmitter transporters (NTTs) control synaptic responses by modulating the concentration of neurotransmitters at cleft. Glutamate is most abundant excitatory neurotransmitter in brain and needs to be finely tuned time space maintain a healthy precise neurotransmission. The glutamate transporter EAAT2 (SLC1A2) primarily responsible for clearance. impairment has been associated with Alzheimer's disease (AD), Huntington's (HD), amyotrophic lateral sclerosis (ALS), Parkinson's (PD). Mutations leucine-rich repeat kinase 2 (LRRK2) contribute both monogenic sporadic forms PD, which common substitution Gly2019Ser significant deficit expression. role pathological mutants LRRK2 intensively studied reviewed. Here we have focused attention on physiological EAAT2, comparing activity NTTs or without kinase. By heterologous expression Xenopus laevis oocytes two-electrode voltage clamp, current amplitudes selected kinetic parameters collected presence absence LRRK2. results show that function are impaired also under its pharmacological inhibition via MLi-2 treatment. stabilizes increasing amount plasma membrane. Interestingly, action EAAT2-specific, as observed no changes transport amplitude obtained other inhibitory studied. This study, first time, demonstrates importance function, highlighting specificity LRRK2-mediated modulation suggesting potential checkpoint preserving neurons from excitotoxicity. In conditions clearance, targeting regulation may offer novel therapeutic opportunities.

Language: Английский

Abnormal Weakening of DNA Methylation around the SLC6A1 Gene Promoter in Temporal Lobe Epilepsy

Journal of Integrative Neuroscience, Journal Year: 2024, Volume and Issue: 23(9)

Published: Sept. 24, 2024

Background: The solute carrier (SLC) superfamily, which transports solutes across biological membranes, includes four members (SLC2A1, SLC6A1, SLC9A64, and SLC35A2) that have been linked to epilepsy. This study sought examine the DNA methylation patterns near promoters of these genes in temporal lobe epilepsy (TLE), as is a crucial epigenetic modification can impact gene expression. Methods: comprised 38 individuals with TLE healthy controls. Methylation experiments were performed using peripheral blood, while demethylation carried out SH-SY5Y cells inhibitor decitabine. Results: A significant difference was observed rate SLC6A1 between patients controls, showing lower (4.81% vs. 5.77%, p = 0.0000), remained even after Bonferroni correction (p 0.0000). Based on hypomethylated TLE, predictive model established showed promise distinguishing calibrating TLE. In group, there differences rates young older controls (4.42% 5.22%, 0.0004). similar trend 0.0436) noted adjusting for sex, age at onset, drug response. addition, found had silencing expression cells, treated decitabine set dose gradient. Conclusions: evidence suggests may stimulate transcription however, further investigation necessary confirm exact mechanism.

Language: Английский