miR-574-5p in epigenetic regulation and Toll-like receptor signaling DOI Creative Commons

James Y. Yang

Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 26, 2024

miR-574-5p is an unusual microRNA (miRNA) that often upregulated or downregulated following exposure to irradiation toxic chemicals; bacterial, parasitic viral infection; and a variety of other disease conditions. Canonically, epigenetically regulates the expression many messenger RNAs (mRNAs) through miRNA-mediated posttranscriptional regulation, thereby affecting cellular physiology pathophysiology contributing pathogenesis progression diseases. However, recent studies have established in addition serving as fine-tuning repressor gene expression, also stimulates endogenous ligand for Toll-like receptor-8/7 (TLR8/7). Indeed, binding TLR8/7 triggers TLR signaling pathway, leading induction interferons, inflammatory cytokines autoimmune signaling. These findings suggest not only important epigenetic regulator but immune responses. Abnormal miR-574-5p-TLR8/7 has been shown be tightly associated with inflammation-related cancers number disorders. can serve potential biomarker Most importantly, promising therapeutic target treatment prevention human disorders, especially infectious diseases,

Language: Английский

Crosstalk between kidney and bones: New perspective for modulating osteoporosis DOI

Yan Duan,

Lijuan Zhao, Yuting Lu

et al.

Ageing Research Reviews, Journal Year: 2025, Volume and Issue: unknown, P. 102776 - 102776

Published: May 1, 2025

Language: Английский

Citations

0
Extracellular Vesicles in Calcium Oxalate Nephrolithiasis: Emerging Biomarkers and Therapeutic Potential DOI Creative Commons
Zhu Wang,

Xu Changzhi,

Qiong Deng

et al.

Advanced NanoBiomed Research, Journal Year: 2025, Volume and Issue: unknown

Published: May 20, 2025

Kidney stone ranks as one of the most prevalent disorders in urology department, causing substantial personal suffering and healthcare costs globally. However, prediction, early diagnosis, treatment kidney disease are still limited. Extracellular vesicles (EVs), loaded with nucleic acids, proteins, metabolites, lipids, released by a wide variety cell types have potential biomarkers for disease. Meanwhile, some natural EVs derived from plants animals been evidenced to effects on elimination calcium oxalate crystals. More importantly, recent explorations elucidated multifaceted role therapeutic applications. These engineered can be RNAs, oligonucleotides, peptides, small molecules; this approach has shown great promise targeted drug delivery presents solution challenges prevention treatment. This review focuses blood, urine, kidney, gut microbiota, urine bacteria, which contribute crystal elimination. The is significant, offering personalized options. it crucial assess moving EV‐based therapies laboratory settings clinical

Language: Английский

Citations

0
CREB1/CRTC2 regulated tubular epithelial-derived exosomal miR-93-3p promotes kidney injury induced by calcium oxalate via activating M1 polarization and macrophage extracellular trap formation DOI Creative Commons

Yushi Sun,

Bojun Li,

Baofeng Song

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 12, 2025

Calcium oxalate (CaOx) crystals are known to cause renal injury and trigger inflammatory responses. However, the role of exosome-mediated epithelial-macrophage communication in CaOx-induced kidney remains unclear. To identify key molecules, miRNA sequencing was conducted on exosomes derived from CaOx-treated (CaOx-exo) control (Ctrl-exo) epithelial cells, identifying miR-93-3p as significantly upregulated. A combination dual-luciferase reporter assays, Western blot, RT-qPCR, immunofluorescence staining, flow cytometry, electrophoretic mobility shift assay (EMSA), chromatin immunoprecipitation-qPCR (CHIP-qPCR) used explore regulation by CREB1/CRTC2 its downstream effects NFAT5/Akt1/NIK/NF-κB2 signaling macrophages. The functional roles NFAT5 macrophage polarization extracellular traps (METs) formation were further evaluated both vitro vivo. Epithelial stimulated CaOx found promote via METs formation. Treatment with NIK SMI1, a inhibitor, or CI-amidine, mitigated crystal deposition damage. Overexpression mouse model reduced deposition, downregulated NF-κB2 levels, decreased number M1-polarized Mechanistic studies revealed that directly targets mRNA, confirmed qRT-PCR, blot. Additionally, we demonstrated acts transcriptional activator miR-93-3p. Inhibition partially reversed NIK/NF-κB2 activation alleviated injury. exacerbate interstitial promoting M1 through CREB1/CRTC2–exosomal miR-93-3p–NIK/NF-κB2 pathway. Targeting this pathway may provide therapeutic avenues for mitigating deposition-induced

Language: Английский

Citations

0
Noncoding RNA, friend or foe for nephrolithiasis? DOI Creative Commons
Qing Wang, Zhenlu Yang, Xiaolong Chen

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Nov. 20, 2024

Nephrolithiasis is one of the most common diseases in urology, characterized by notable incidence and recurrence rates, leading to significant morbidity financial burden. Despite its prevalence, precise mechanisms underlying stone formation remain incompletely understood, thus hindering advancements kidney management over past three decades. Investigating pivotal biological molecules that govern has consistently been a challenging high-priority task. A portion mammalian genomes are transcribed into noncoding RNAs (ncRNAs), which have ability modulate gene expression disease progression. They emerging as novel target class for diagnostics pharmaceutical exploration. In recent years, role ncRNAs attracted burgeoning attention. found influence regulating ion transportation, oxidative stress injury, inflammation, osteoblastic transformation, autophagy, pyroptosis. These findings contributes new perspectives on pathogenesis nephrolithiasis. To enhance our understanding diagnostic therapeutic potential nephrolithiasis-associated ncRNAs, we summarized profiles, functions, clinical significance these current review.

Language: Английский

Citations

0
miR-574-5p in epigenetic regulation and Toll-like receptor signaling DOI Creative Commons

James Y. Yang

Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 26, 2024

miR-574-5p is an unusual microRNA (miRNA) that often upregulated or downregulated following exposure to irradiation toxic chemicals; bacterial, parasitic viral infection; and a variety of other disease conditions. Canonically, epigenetically regulates the expression many messenger RNAs (mRNAs) through miRNA-mediated posttranscriptional regulation, thereby affecting cellular physiology pathophysiology contributing pathogenesis progression diseases. However, recent studies have established in addition serving as fine-tuning repressor gene expression, also stimulates endogenous ligand for Toll-like receptor-8/7 (TLR8/7). Indeed, binding TLR8/7 triggers TLR signaling pathway, leading induction interferons, inflammatory cytokines autoimmune signaling. These findings suggest not only important epigenetic regulator but immune responses. Abnormal miR-574-5p-TLR8/7 has been shown be tightly associated with inflammation-related cancers number disorders. can serve potential biomarker Most importantly, promising therapeutic target treatment prevention human disorders, especially infectious diseases,

Language: Английский

Citations

0