Expression of both N- and C-terminal GFP tagged huCD36 and their discrepancy in OxLDL and pRBC binding on CHO cells DOI Creative Commons
Jianshe Zhang,

Ian Crandall

Lipids in Health and Disease, Journal Year: 2007, Volume and Issue: 6(1), P. 24 - 24

Published: Jan. 1, 2007

CD36, an 88 kDa membrane glycoprotein, is found in several cell types and it has been characterized to have two hydrophobic domains at their N- C-termini which are essential for protein folding targeting. In this study, we first tagged the green fluorescent (GFP) both of huCD36 investigated cellular expression influences on lipoprotein plasmodium falciparium parasitized erythrocytes (pRBC) binding. Our work revealed that proteins expressed normally irrespective GFP tag presence either or C-termini. However, recombinant showed discrepancy uptake surface-binding OxLDL but they did not affect pRBC These results suggested interaction between oxLDL CD36 could be blocked using may useful potential control trafficking modified lipoproteins into monocytes leading atherogenesis.

Language: Английский

High-density lipoprotein and transport of cholesterol and triglyceride in blood DOI
W. Virgil Brown

Journal of clinical lipidology, Journal Year: 2007, Volume and Issue: 1(1), P. 7 - 19

Published: March 1, 2007

Language: Английский

Citations

42
Increased risk of cardiovascular disease in Type 1 diabetes: arterial exposure to remnant lipoproteins leads to enhanced deposition of cholesterol and binding to glycated extracellular matrix proteoglycans DOI

Rabban Mangat,

Jenny Su,

Jessica E. Lambert

et al.

Diabetic Medicine, Journal Year: 2010, Volume and Issue: 28(1), P. 61 - 72

Published: Sept. 27, 2010

Diabet. Med. 28, 61–72 (2011) Abstract Aims To determine fasting and postprandial metabolism of apolipoprotein B48 (apoB48) remnant lipoproteins in subjects with Type 1 diabetes the relationship to progressive cardiovascular disease, investigate impact lipoprotein cholesterol accumulation associated arterial wall biglycan using a rodent model diabetes. Methods Normolipidaemic ( n = 9) long‐standing (and advanced disease) seven healthy control were studied. Fasting apoB48 concentration was determined following sequential meal challenge. A streptozotocin‐induced used ex vivo retention fluorescent‐conjugated remnants. Binding human recombinant assessed vitro . Results significantly higher plasma remnants observed patients compared subjects. Patients exhibited greater total area under curve (AUC) an increased incremental AUC second The sevenfold rats relative controls. Remnants shown bind significant affinity further 2.3‐fold binding capacity glycated biglycan. colocalize both matrix proteins model. Conclusion Impaired enhanced proteoglycans appears contribute deposition Our findings support hypothesis that impaired may accelerated progression atherosclerosis hyperglycaemic insulin‐deficient state.

Language: Английский

Citations

35
Influence of chylomicron remnants on human monocyte activation in vitro DOI Creative Commons

Carolyn Bentley,

N. Hathaway,

J. Widdows

et al.

Nutrition Metabolism and Cardiovascular Diseases, Journal Year: 2010, Volume and Issue: 21(11), P. 871 - 878

Published: Aug. 3, 2010

Atherosclerosis is known to be an inflammatory disease and there increasing evidence that chylomicron remnants (CMR), the lipoproteins which carry dietary fats in blood, cause macrophage foam cell formation inflammation. In early atherosclerosis frequency of activated monocytes peripheral circulation increased, clearance CMR from blood may delayed, however, whether contribute directly monocyte activation subsequent egress into arterial wall has not been established. Here, contribution pro-inflammatory pathways was assessed using vitro model.Primary human CMR-like particles (CRLP) were used measure several endpoints activation. Treatment with CRLP caused rapid prolonged generation reactive oxygen species by monocytes. The chemokines MCP-1 IL-8 secreted nanogram quantities cells absence CRLP. secretion transiently increased after treatment, maintained presence pharmacological inhibitors production. contrast, exposure significantly reduced secretion. Chemotaxis towards pre-exposed reversed addition exogenous MCP-1.Our findings indicate activate augment their migration reducing cellular expression. Our data support current hypothesis milieu atherosclerosis, suggest this reflect direct interaction circulating

Language: Английский

Citations

34
Karma of Cardiovascular Disease Risk Factors for Prevention and Management of Major Cardiovascular Events in the Context of Acute Exacerbations of Chronic Obstructive Pulmonary Disease DOI Creative Commons

Liliana Crisan,

Nathan D. Wong, Don D. Sin

et al.

Frontiers in Cardiovascular Medicine, Journal Year: 2019, Volume and Issue: 6

Published: June 25, 2019

Cardiovascular diseases (CVDs) and chronic obstructive pulmonary disease (COPD) are the two leading causes of mortality morbidity worldwide that coexist frequently. Most recent studies have concluded patients with CVD or risk factors for more likely to major adverse cardiovascular events (MACEs) after an acute exacerbation COPD, severe COPD higher than those less COPD. The MACEs in is determined by complex interactions between genetics, behavioral, metabolic environmental factors. To date, there no guidelines regarding prevention, screening, management modifiable context exacerbations, insufficient control It not clear whether tight individual (e.g., smoking cessation) composite will reduce deaths from cardiac events. A deeper insight may improve strategies reduction Key words: Chronic disease, diseases, events,

Language: Английский

Citations

19
Genetics of Triglyceride-Rich Lipoproteins Guide Identification of Pharmacotherapy for Cardiovascular Risk Reduction DOI
Aleesha Shaik,

Robert S. Rosenson

Cardiovascular Drugs and Therapy, Journal Year: 2021, Volume and Issue: 35(3), P. 677 - 690

Published: March 12, 2021

Language: Английский

Citations

14
Metabolic disorders and inflammation are associated with familial combined hyperlipemia DOI

M. J. Díaz-Ruíz,

María L. Martínez-Triguero,

Antonio López‐Ruiz

et al.

Clinica Chimica Acta, Journal Year: 2018, Volume and Issue: 490, P. 194 - 199

Published: Sept. 7, 2018

Language: Английский

Citations

15
Impaired ATP‐binding cassette transporter A1‐mediated sterol efflux from oxidized LDL‐loaded macrophages DOI
Elda Favari, Francesca Zimetti, Anna E. Bortnick

et al.

FEBS Letters, Journal Year: 2005, Volume and Issue: 579(29), P. 6537 - 6542

Published: Nov. 2, 2005

We investigated the interaction of oxidized low density lipoprotein (OxLDL) with ATP‐binding cassette A1 (ABCA1) pathway in J774 macrophages. Cellular efflux to apolipoprotein AI (apo‐AI) OxLDL‐derived cholesterol was lower than derived from acetylated (AcLDL). ABCA1 upregulation by 8‐(4‐chlorophenylthio)adenosine 3′:5′‐cyclic monophosphate (cpt‐cAMP) or 22 (R)‐hydroxycholesterol (22‐OH) and 9‐ cis retinoic acid (9cRA) increased apo‐AI cellular sterols AcLDL, but not those OxLDL. OxLDL, induced protein content activity J774. However, OxLDL did influence cpt‐cAMP 22‐OH/9cRA. conclude that released cells are available neither as substrate nor modulator ABCA1.

Language: Английский

Citations

24
Probucol mediates vascular remodeling after percutaneous transluminal angioplasty via down-regulation of the ERK1/2 signaling pathway DOI
Yunbo Yang, Yixin Yang, Bo Su

et al.

European Journal of Pharmacology, Journal Year: 2007, Volume and Issue: 570(1-3), P. 125 - 134

Published: June 11, 2007

Language: Английский

Citations

18
Oxidation of chylomicron remnant-like particles inhibits their uptake by THP-1 macrophages by apolipoprotein E-dependent processes DOI Creative Commons

Fatos Bejta,

Elizabeth Hernández Moore,

Michael Avella

et al.

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Journal Year: 2007, Volume and Issue: 1771(7), P. 901 - 910

Published: May 4, 2007

The influence of the oxidative state chylomicron remnants (CMR) on mechanisms their uptake and induction lipid accumulation by macrophages derived from human monocyte cell line, THP-1, during foam formation was investigated using chylomicron-remnant-like particles (CRLPs) at 3 different levels oxidation. CRLPs varied exposure to CuSO(4) (oxCRLPs) or incorporation antioxidant, probucol (pCRLPs) into particles. oxCRLPs caused significantly less triacylglycerol in than CRLPs, rate lower, while pCRLPs more were taken up faster. Uptake all types inhibited a similar extent when entry via low density lipoprotein (LDL) receptor related protein (80-90%), LDL (-30-40%), CD36 (-40%) phagocytosis (-35-40%) blocked lactoferrin, excess LDL, anti-CD36 cytochalasin D, respectively, but blocking scavenger receptors-A -B1 poly inosinic acid HDL had no effect. These findings show that oxidation lowers macrophages. However, does not change main pathways internalisation THP-1 macrophages, which occur mainly LRP with some contribution LDLr, have only minor role, regardless Thus, effects CMR contrast sharply those this may be important role dietary oxidized lipids antioxidants modulating atherosclerosis.

Language: Английский

Citations

18
Modulation of Macrophage Fatty Acid Content and Composition by Exposure to Dyslipidemic Serum in Vitro DOI
Bruce X. Wong,

Reece A. Kyle,

Kevin D. Croft

et al.

Lipids, Journal Year: 2011, Volume and Issue: 46(4), P. 371 - 380

Published: Jan. 31, 2011

Abstract Macrophages in arterial walls accumulate lipids leading to the development of atherosclerotic plaques. However, mechanisms underlying macrophage lipid accumulation and foam cell formation are often studied without accounting for risk factors such as dyslipidemia. We investigated effect varying concentrations triglyceride (TG) within physiological range on fatty acid (FA) expression cholesterol efflux proteins. Human monocytes were cultured media supplemented with 10% sera containing low (0.7 mmol/L) high (1.4 TG. The resulting macrophages harvested after 10 days analysis FA content composition genes involved metabolism. Exposure higher TG lower HDL increased content. exposed had total compared controls (876 μg/mg protein vs. 652 protein) greater proportions C16:0, C18:1 C18:2. Macrophage both ABCA1 ABCG1 proteins reduced when present media. Expression scavenger receptor CD36, lipoprotein uptake, was also downregulated Culturing conditions versus influenced composition, levels regulatory Replicating vitro dyslipidemia encountered vivo may provide an informative model investigation atherogenesis.

Language: Английский

Citations

13