Assessment of Allometry in Environmental DNA and RNA Production From Ayu (Plecoglossus altivelis) in an Experimental Condition Using Mitochondrial and Nuclear Gene Markers DOI Creative Commons
Toshiaki Jo,

Yusuke Ozaki,

Nao Matsuda

et al.

Environmental DNA, Journal Year: 2024, Volume and Issue: 6(6)

Published: Nov. 1, 2024

ABSTRACT Recent studies demonstrated that integrating allometric scaling could strengthen the relationship between environmental DNA (eDNA) concentration and organism abundance. The finding has been supported mainly by field surveys but not verified sufficiently at individual level in a controlled experimental condition. In addition, it remains unknown whether only eDNA also RNA (eRNA) production scales allometrically with body mass. To address knowledge gaps, we conducted long‐term rearing experiment using ayu ( Plecoglossus altivelis ) to monitor of their messenger ribosomal eRNA growth from larvae adults. Water samples were collected tanks P. (eNAs) concentrations water estimated quantitative real‐time PCR mitochondrial nuclear gene markers. parallel each sampling, number fish individuals total biomass tank recorded. Regardless types, individual‐level eNA related scaled mass (ASM), coefficient b = 0.75, more strongly than mean result indicated larger produces lesser mass‐specific particles, supporting relevance population observed surveys. Moreover, ratios some significantly decreased tank. may relate changes intracellular physiology and/or aging, having potential indicate population's average size. Our study offered insights into various particles depending on size metabolic rate implications for better understanding ecology effective stock management.

Language: Английский

Biodiversity Monitoring in Remote Marine Environments: Advancing Environmental DNA/RNA Sampling Workflows DOI Creative Commons
Michelle Scriver, Anastasija Zaiko, Xavier Pochon

et al.

Marine Environmental Research, Journal Year: 2025, Volume and Issue: 206, P. 107041 - 107041

Published: Feb. 26, 2025

Language: Английский

Citations

0
Parameterizing the particle size distribution of environmental DNA provides insights into its improved availability from the water DOI
Toshiaki Jo

Environmental Monitoring and Assessment, Journal Year: 2025, Volume and Issue: 197(5)

Published: April 8, 2025

Language: Английский

Citations

0
Assessment of Allometry in Environmental DNA and RNA Production From Ayu (Plecoglossus altivelis) in an Experimental Condition Using Mitochondrial and Nuclear Gene Markers DOI Creative Commons
Toshiaki Jo,

Yusuke Ozaki,

Nao Matsuda

et al.

Environmental DNA, Journal Year: 2024, Volume and Issue: 6(6)

Published: Nov. 1, 2024

ABSTRACT Recent studies demonstrated that integrating allometric scaling could strengthen the relationship between environmental DNA (eDNA) concentration and organism abundance. The finding has been supported mainly by field surveys but not verified sufficiently at individual level in a controlled experimental condition. In addition, it remains unknown whether only eDNA also RNA (eRNA) production scales allometrically with body mass. To address knowledge gaps, we conducted long‐term rearing experiment using ayu ( Plecoglossus altivelis ) to monitor of their messenger ribosomal eRNA growth from larvae adults. Water samples were collected tanks P. (eNAs) concentrations water estimated quantitative real‐time PCR mitochondrial nuclear gene markers. parallel each sampling, number fish individuals total biomass tank recorded. Regardless types, individual‐level eNA related scaled mass (ASM), coefficient b = 0.75, more strongly than mean result indicated larger produces lesser mass‐specific particles, supporting relevance population observed surveys. Moreover, ratios some significantly decreased tank. may relate changes intracellular physiology and/or aging, having potential indicate population's average size. Our study offered insights into various particles depending on size metabolic rate implications for better understanding ecology effective stock management.

Language: Английский

Citations

0