Chromosome-scale genome assembly of bread wheat’s wild relativeTriticum timopheevii DOI Creative Commons
Surbhi Grewal, Caiyun Yang,

Duncan Scholefield

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 17, 2024

Abstract Wheat ( Triticum aestivum ) is one of the most important food crops with an urgent need for increase in its production to feed growing world. timopheevii (2n = 4x 28) allotetraploid wheat wild relative species containing A t and G genomes that has been exploited many pre-breeding programmes improvement. In this study, we report generation a chromosome-scale reference genome assembly T. accession PI 94760 based on PacBio HiFi reads chromosome conformation capture (Hi-C). The comprised total size 9.35 Gb, featuring contig N50 42.4 Mb, 166,325 predicted gene models. DNA methylation analysis showed had average more methylated bases than genome. was also closely related S Aegilops speltoides B hexaploid or tetraploid wheat. summary, provides valuable resource genome-informed discovery agronomically genes security.

Language: Английский

QTL analysis of spike traits and KASP marker validation for basal sterile spikelet number in wheat DOI Creative Commons

Pengbo Song,

Yueyue Li, Kaoping Song

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 10, 2025

Spike traits play a pivotal role in determining wheat yield. In this study, two recombinant inbred line (RIL) populations were employed to identify quantitative trait loci (QTLs) for spike length (SL), spikelet number per (SNS), and basal sterile (BSSN). A total of 30 QTLs identified, including 7 major-effect that exhibited stability across multiple environments. Among these, novel discovered: QBssn.A-3 A.3 QBssn.AV-5B.2. Furthermore, it was found the combination these with other major effect had no impact on thousand-kernel weight (TKW). For A.3, Kompetitive Allele-Specific PCR (KASP) marker developed validated based its physical location. Additionally, candidate genes within intervals QBssn.AV-5B.2 predicted, primarily associated spike. This study provides foundation future map-baesd cloning related traits, breeding high-yield varieties.

Language: Английский

Citations

0
Identification and validation of a novel tiller inhibition locus (tin7) on chromosome 2BL in wheat DOI
Shuai Hou,

Yuzhou Mou,

Haojie Li

et al.

Molecular Breeding, Journal Year: 2025, Volume and Issue: 45(5)

Published: May 1, 2025

Language: Английский

Citations

0
Enhancing the breeding gene pool of wheat using accessions in gene banks as demonstrated by the Watkins collection DOI

Demissew Sertse,

Mulualem T. Kassa, Brent McCallum

et al.

Theoretical and Applied Genetics, Journal Year: 2025, Volume and Issue: 138(6)

Published: May 5, 2025

Language: Английский

Citations

0
A diagnostic marker for the Hessian fly resistance gene H13 in wheat DOI
Xiaoting Xu,

Xuming Liu,

Ming‐Shun Chen

et al.

Crop Science, Journal Year: 2025, Volume and Issue: 65(3)

Published: May 1, 2025

Abstract Hessian fly [HF; Mayetiola destructor (Say)] is a serious pest of wheat in the United States, and growing HF‐resistant cultivars an effective approach to control pest. H13 from Aegilops tauschii resistance gene on chromosome 6D that shows high multiple HF biotypes; however, diagnostic markers are not available for selection gene. This study developed two kompetitive allele‐specific PCR (KASP) markers, Kasp_H13_R1064* Kasp_H13_R217K , using sequence previously reported candidate Both showed same segregation pattern Molly × Newton population 38 lines carry different named genes. The marker alleles were detected ( ), KS89WGRC03 H23 KS89WGRC04 Hwgrc4 ) with genes all mapped arm 6DS A. . results suggest those near three they either allelic or tightly linked. was validated diversity panel major US wheat‐growing areas carrying Further screening breeding 2022 hard winter (HWW) regional nurseries (RGON2022 RPN2022; where RGON Regional Germplasm Observation Nursery RPN Performance Nursery) did detect suggesting has been deployed current HWW programs. allele at also relatively low (16.1%) worldwide collection. Development KASP will facilitate deployment

Language: Английский

Citations

0
Allelic Variations in Vernalization (Vrn) Genes in Triticum spp. DOI Open Access
Sanaz Afshari-Behbahanizadeh, Damiano Puglisi, Salvatore Esposito

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(2), P. 251 - 251

Published: Feb. 17, 2024

Rapid climate changes, with higher warming rates during winter and spring seasons, dramatically affect the vernalization requirements, one of most critical processes for induction wheat reproductive growth, severe consequences on flowering time, grain filling, yield. Specifically, Vrn genes play a major role in transition from vegetative to growth wheat. Recent advances genomics have significantly improved understanding molecular mechanisms (Vrn-1, Vrn-2, Vrn-3, Vrn-4), unveiling diverse array natural allelic variations. In this review, we examined current knowledge functional structural point view, considering studies conducted alleles at different ploidy levels (diploid, tetraploid, hexaploid). The characterization Vrn-1 has been focal point, revealing forms implications time. We highlighted complexity problems linked nomenclature some alleles. Addressing these issues will be crucial harmonizing research efforts enhancing our gene function evolution. increasing availability genome transcriptome sequences, along improvements bioinformatics computational biology, offers versatile range possibilities enriching genomic regions surrounding target sites genes, paving way innovative approaches manipulate time improve productivity.

Language: Английский

Citations

3
The Expression of Triticum aestivum Cysteine-Rich Receptor-like Protein Kinase Genes during Leaf Rust Fungal Infection DOI Creative Commons
Ahmed Kamel, Khaled Metwally, Mostafa Sabry

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(16), P. 2932 - 2932

Published: Aug. 14, 2023

Understanding the role of cysteine-rich receptor-like kinases (CRKs) in plant defense mechanisms is crucial for enhancing wheat resistance to leaf rust fungus infection. Here, we identified and verified 164 members CRK gene family using Triticum aestivum reference version 2 collected from international genome sequencing consortium (IWGSC). The proteins exhibited characteristic features CRKs, including presence signal peptides, cysteine-rich/stress antifungal/DUF26 domains, transmembrane Pkinase domains. Phylogenetic analysis revealed extensive diversification within family, indicating development distinct specific functional roles plants. When studying expression near-isogenic lines (NILs) carrying Lr57- Lr14a-resistant genes, Puccinia triticina, causal agent fungus, triggered temporal dynamics. upregulation genes resistant interaction indicated their potential rust, while contrasting patterns susceptible highlighted susceptibility associated genes. study uncovered certain that upon infection gene. findings suggest targeting CRKs may present a promising strategy improving diseases.

Language: Английский

Citations

7
Identifying the seeds of heterotic pools for Southern and Eastern Africa from global elite spring wheat germplasm DOI Creative Commons

Carus John-Bejai,

Richard Trethowan,

Isobella Revell

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: June 27, 2024

Hybrid breeding can increase the competitiveness of wheat (Triticum aestivum L.) in Sub-Saharan Africa by fostering more public-private partnerships and promoting investment private sector. The benefit hybrid cultivars South has previously been demonstrated but due to high cost seed production, not received significant attention past decade. Considering renewed commitment sector establish as a crop globally, coupled with research into enhancement outcrossing wheat, Southern Eastern should be revisited. Our study aimed identify genetically distinct germplasm groups spring that would useful establishment heterotic pools targeting this region. Multi-environment yield testing large panel F1 test hybrids, generated using global elite germplasm, was carried out between 2019 2020 Argentina, Africa, Europe, Australia. We observed genotype environment interactions within our network, confirming distinctiveness African trial sites. Relatively additive genetic variance highlighting contribution parental genotypes grain hybrids. explored architecture these parents factors underlying value appear associated their subgroup, positive marker effects distributed throughout genome. In testcrosses, from International Maize Wheat Improvement Center (CIMMYT) complementary bred Africa. feasibility achieving gain via pool divergence, extension viability is supported results study.

Language: Английский

Citations

2
The addition of Psathyrostachys Huashanica Keng 6Ns large segment chromosomes has positive impact on stripe rust resistance and plant spikelet number of common wheat DOI Creative Commons
Jiaojiao Li,

Jiachuang Li,

Xueni Cheng

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: July 18, 2024

Abstract Background Developing novel germplasm by using wheat wild related species is an effective way to rebuild the resource bank. The Psathyrostachys huashanica Keng ( P. , 2 n = x 14, NsNs) regarded as a superior improve breeding because of its multi-resistance, early maturation and numerous tiller traits. Introducing genetic components into common background most important step in achieving use. Therefore, cytogenetic characterization influence introgressed large segment chromosomes necessary be explored. Results In this study, we characterized derived line, named D88-2a, progeny former wheat- partial amphiploid line H8911 (2 7 49, AABBDDNs). Cytological identification showed that chromosomal composition D88-2a was 44 22II, indicating addition exogenous chromosomes. Genomic situ hybridization demonstrated supernumerary were pair homologues from could stably inherited background. Molecular markers 15 K SNP array indicated additional sixth homoeologous group (i.e., 6Ns) . Based on distribution heterozygous single-nucleotide polymorphism sites fluorescence karyotype each chromosome, confirmed 6Ns chromosomes, which contained entire short arm proximal centromere portion long arm. terms agronomic traits, exhibited enhanced stripe rust resistance, improved spike characteristics increased protein content than parent 7182. Conclusions new cytogenetically stable alien chromosome has positive impact plant spikelet number resistance. Thus, can used for improvement cultivated study functional segment.

Language: Английский

Citations

2
Cytogenetic identification and molecular mapping for the wheat-Thinopyrum ponticum introgression line with resistance to Fusarium head blight DOI
Xiaoying Yang, Xiaofang Cheng, Guangyi Wang

et al.

Theoretical and Applied Genetics, Journal Year: 2024, Volume and Issue: 137(8)

Published: July 24, 2024

Language: Английский

Citations

2
Genome-wide characterization and expression analysis of the CINNAMYL ALCOHOL DEHYDROGENASE gene family in Triticum aestivum DOI Creative Commons
Luigi M. Peracchi, Rhoda A. T. Brew‐Appiah, Kim Campbell

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Aug. 29, 2024

CINNAMYL ALCOHOL DEHYDROGENASE (CAD) catalyzes the NADPH-dependent reduction of cinnamaldehydes into cinnamyl alcohols and is a key enzyme found at final step monolignol pathway. Cinnamyl their conjugates are subsequently polymerized in secondary cell wall to form lignin. CAD genes typically encoded by multi-gene families thus traditionally organized general classifications functional relevance.

Language: Английский

Citations

2