Rapid isolation of Yr9 via MutIsoSeq and QTL analysis of durable stripe rust resistance in wheat cultivar Xingzi 9104 DOI Creative Commons
Yibo Zhang, Shuo Huang, Yuqing Li

et al.

Stress Biology, Journal Year: 2025, Volume and Issue: 5(1)

Published: April 30, 2025

Abstract The fungus Puccinia striiformis f. sp. tritici ( Pst ) is the causal agent of wheat stripe rust which constitutes a major limitation to production. Cloning and applying disease-resistant genes are considered as an effective solution. Chinese cultivar Xingzi 9104 (XZ9104) has exhibited durable resistance across multiple environments since its release. Through quantitative trait loci (QTL) analysis, eight QTL were found on chromosome arms 1BS, 1BL, 2AL, 2BL, 3BS, 4BL, 5BL 7BL. YrXZ identified 1RS.1BL translocation conferred race-specific all-stage race CYR23. QYrxz.nwafu-1BL.6 QYrxz.nwafu-3BS.7 adult plant Yr29 Yr30 , respectively. Notably, QYrxz.nwafu-2BL.5 accounted for 15.75–47.63% phenotypic variation diverse pyramiding with can confer high level resistance. Other environment-dependent minor effects. To clone above genes, we created population over 2,000 M 5 mutants in XZ9104 using ethylmethane sulfonate (EMS) mutagenesis screened various types susceptible mutants. Using MutIsoseq approach five mutant lines CYR23, rapid isolated candidate gene encoding coiled-coil nucleotide-binding site leucine-rich repeat (CC-NBS-LRR) protein. Integrating cytological gene-based association transcriptomic profiling virus-induced silencing (VIGS), confirmed that was indeed Yr9. This study demonstrated different effects contributed XZ9104. Understanding molecular mechanisms pathways involved defense inform future strategies deploying engineering genetic against evolving diseases.

Language: Английский

Rapid isolation of Yr9 via MutIsoSeq and QTL analysis of durable stripe rust resistance in wheat cultivar Xingzi 9104 DOI Creative Commons
Yibo Zhang, Shuo Huang, Yuqing Li

et al.

Stress Biology, Journal Year: 2025, Volume and Issue: 5(1)

Published: April 30, 2025

Abstract The fungus Puccinia striiformis f. sp. tritici ( Pst ) is the causal agent of wheat stripe rust which constitutes a major limitation to production. Cloning and applying disease-resistant genes are considered as an effective solution. Chinese cultivar Xingzi 9104 (XZ9104) has exhibited durable resistance across multiple environments since its release. Through quantitative trait loci (QTL) analysis, eight QTL were found on chromosome arms 1BS, 1BL, 2AL, 2BL, 3BS, 4BL, 5BL 7BL. YrXZ identified 1RS.1BL translocation conferred race-specific all-stage race CYR23. QYrxz.nwafu-1BL.6 QYrxz.nwafu-3BS.7 adult plant Yr29 Yr30 , respectively. Notably, QYrxz.nwafu-2BL.5 accounted for 15.75–47.63% phenotypic variation diverse pyramiding with can confer high level resistance. Other environment-dependent minor effects. To clone above genes, we created population over 2,000 M 5 mutants in XZ9104 using ethylmethane sulfonate (EMS) mutagenesis screened various types susceptible mutants. Using MutIsoseq approach five mutant lines CYR23, rapid isolated candidate gene encoding coiled-coil nucleotide-binding site leucine-rich repeat (CC-NBS-LRR) protein. Integrating cytological gene-based association transcriptomic profiling virus-induced silencing (VIGS), confirmed that was indeed Yr9. This study demonstrated different effects contributed XZ9104. Understanding molecular mechanisms pathways involved defense inform future strategies deploying engineering genetic against evolving diseases.

Language: Английский

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