Comprehensive analysis of housekeeping genes, tissue-specific genes, and dynamic regulation across developmental stages in pearl millet DOI Creative Commons
Wei Luo, Min Sun, Ailing Zhang

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Dec. 18, 2024

Abstract Background Pearl millet ( Pennisetum glaucum (L.) R. Br.) is a vital cereal crop, predominantly cultivated in arid and semi-arid regions of Asia Africa. It serves as staple food for millions, while also being utilized forage an energy crop. The crop’s resistance to heat drought, coupled with its high biomass, positions it promising candidate climate-resilient agriculture. A detailed understanding gene expression patterns across various tissues developmental stages essential enhancing yield quality. This study aims fill this knowledge gap by employing RNA-seq identify housekeeping genes (HKGs) tissue-specific (TSGs) pearl millet. Results Our analysis data from nine (seed, germ, radicle, leaf, root, tillering tissue, stem, spike, grain) eight accession Tifleaf3 revealed comprehensive profile. We identified 461 HKGs that exhibited stable all stages, providing robust internal references RT-qPCR. Additionally, 8091 TSGs were discovered, many which showed distinctive such leaf. Functional enrichment these using GO KEGG pathways highlighted their roles key biological processes pathways, indicating potential crop trait enhancement. Protein-protein interaction networks constructed stem leaf further illuminated the regulatory mechanisms underlying transition vegetative reproductive growth stages. Conclusion presents transcriptomic landscape millet, identifying set are crucial molecular basis development. provided valuable options transcript normalization targets exploring function plant development insights gained work instrumental breeding programs aimed at productivity thereby contributing security.

Language: Английский

Development of Genome-Wide SSR Markers in Leymus chinensis with Genetic Diversity Analysis and DNA Fingerprints DOI Open Access

Taiyou Ou,

Zinian Wu,

Chunyu Tian

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 918 - 918

Published: Jan. 22, 2025

Leymus chinensis, a major component of the plant community in eastern Eurasian grasslands with wide distribution, provides stability to grassland ecosystems and supports animal husbandry. This study aimed bridge gap between molecular breeding industrial application L. chinensis by conducting comprehensive simple sequence repeat (SSR) analysis. A total 973,129 SSRs were identified whole genome, which was used design 20 polymorphic pairs SSR primers further assess 105 accessions. On average, 33.55 alleles detected per locus, an average Shannon index 2.939 information content value 0.910. Principal coordinate, maximum likelihood, structure analyses consistently showed that all samples coincidentally divided into four subclasses. In addition, Mantel test data indicated weak correlation genetic geographical distances whose variability may be related pollination mode natural selection pressures. Finally, we selected markers scan accessions, constructing fingerprint for them. These findings provide new foundations identifying superior varieties, improving management resources, germplasm resource database chinensis.

Language: Английский

Citations

0
Phylogenomic analysis and SNP fingerprinting construction of Camellia oleifera Abel. germplasm resources using SLAF-seq technology DOI Creative Commons

Xuming Dan,

Xiner Qumu,

Qiang Lai

et al.

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 229, P. 120978 - 120978

Published: April 8, 2025

Language: Английский

Citations

0
Transcriptomics and metabolomics analyses reveal pollen abortion mechanism in alfalfa early stage male sterile lines DOI Creative Commons
Hanyang Cai,

Shuhe Zhang,

Weijie Yu

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 17, 2024

Alfalfa (

Language: Английский

Citations

1
Comprehensive analysis of housekeeping genes, tissue-specific genes, and dynamic regulation across developmental stages in pearl millet DOI Creative Commons
Wei Luo, Min Sun, Ailing Zhang

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Dec. 18, 2024

Abstract Background Pearl millet ( Pennisetum glaucum (L.) R. Br.) is a vital cereal crop, predominantly cultivated in arid and semi-arid regions of Asia Africa. It serves as staple food for millions, while also being utilized forage an energy crop. The crop’s resistance to heat drought, coupled with its high biomass, positions it promising candidate climate-resilient agriculture. A detailed understanding gene expression patterns across various tissues developmental stages essential enhancing yield quality. This study aims fill this knowledge gap by employing RNA-seq identify housekeeping genes (HKGs) tissue-specific (TSGs) pearl millet. Results Our analysis data from nine (seed, germ, radicle, leaf, root, tillering tissue, stem, spike, grain) eight accession Tifleaf3 revealed comprehensive profile. We identified 461 HKGs that exhibited stable all stages, providing robust internal references RT-qPCR. Additionally, 8091 TSGs were discovered, many which showed distinctive such leaf. Functional enrichment these using GO KEGG pathways highlighted their roles key biological processes pathways, indicating potential crop trait enhancement. Protein-protein interaction networks constructed stem leaf further illuminated the regulatory mechanisms underlying transition vegetative reproductive growth stages. Conclusion presents transcriptomic landscape millet, identifying set are crucial molecular basis development. provided valuable options transcript normalization targets exploring function plant development insights gained work instrumental breeding programs aimed at productivity thereby contributing security.

Language: Английский

Citations

0