Genomic analysis and process optimization for nigericin production in a newly-isolated antimicrobial Streptomyces

New Biotechnology, Journal Year: 2025, Volume and Issue: 86, P. 97 - 106

Published: Feb. 17, 2025

Streptomyces, the dominant species of actinomycetes, contribute to a large proportion bioactive natural products. In our previous study, over 2,400 culture extracts were subjected bioassays against Candida albicans and Staphylococcus aureus. Among these, Streptomyces javensis named Inha503, was found produce nigericin, which exhibits antimicrobial activity gram-positive bacteria, such as Micrococcus luteus S. This study aimed understand genomic features Inha503 optimize downstream process improve titer nigericin derivatives. Identification biosynthetic gene cluster (BGC) in chromosome confirmed using whole-genome bioinformatics core BGC knockout results. The cultivation time optimized 10 days, derivatives identified quantified LC/MS. seven-production media for highest production obtained R5 medium, exhibited 1.75-fold increase compared conditions. Additionally, extraction ethyl acetate single solvent mixture ethanol 7:3 ratio. medium after days with ethanol, implying that purification processes genetic insights promising platform compound nigericin.

Language: Английский

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Discovery of phenoxazine congeners as novel α-glucosidase inhibitors and identification of their biosynthetic gene cluster from Streptomyces sp. CB00316

Archives of Microbiology, Journal Year: 2025, Volume and Issue: 207(6)

Published: April 29, 2025

Language: Английский

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Discovery of Novel D/L-Camphor Derivatives Containing Oxime Ester as Fungicide Candidates: Antifungal Activity, Structure-Activity Relationship and Preliminary Mechanistic Study

Published: Jan. 1, 2024

Language: Английский

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Discovery of Novel D/L-Camphor Derivatives Containing Oxime Ester as Fungicide Candidates: Antifungal Activity, Structure-Activity Relationship and Preliminary Mechanistic Study

Advanced Agrochem, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

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Bioprotection by Natural Sources for Sustainable Agriculture

IntechOpen eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 15, 2024

Biocontrol, or protection, involves using living organisms such as microbes, insects, and their by-products to safeguard food security against harmful pathogens pests in agriculture. Excessive use of synthetic inputs fertilizers pesticides farming can lead soil accumulation crust formation over time. Inorganic chemical pollutants enter the chain through plant absorption from soil, resulting environmental public health concerns. Compensating for destructive effects on agroecosystems is challenging. Therefore, it important discuss future restoration microbial communities phyllosphere, endosphere, rhizosphere, growth–promoting microorganisms natural sources. Local governments should enforce legal restrictions frequency quantity conventional pesticide use, promote bioagents, encourage farmers stakeholders adopt alternatives. This chapter introduces various bio-based materials that serve alternatives chemicals eradicating minimizing invasive phytopathogen species biocontrol protection.

Language: Английский

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Design and synthesis of camphor‐thiazole derivatives as potent antifungal agents: structure–activity relationship and preliminary mechanistic study

Pest Management Science, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

Plant pathogenic fungi pose a severe threat to crop yield and food security. This study aims investigate the potential antifungal activity mechanism of action camphor-thiazole derivatives against six plant fungi. A novel series were designed, synthesized evaluated for their effects Rhizoctonia solani, Fusarium graminearum, Valsa mali, Alternaria Colletotrichum orbiculare Botryitis cinerea.

Language: Английский

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