Anticariogenic activity of marine brown algae Padina boergesenii and its active components towards Streptococcus mutans DOI Creative Commons

Ravichellam Sangavi,

Nambiraman Malligarjunan,

Lakkakula Satish

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Nov. 25, 2024

Streptococcus mutans is a well-recognized bacterium that plays predominant role in the progression of dental caries. Its pathogenicity linked to several key characteristics, including ability produce organic acids (acidogenicity), thrive low pH environments (aciduricity), synthesize exopolysaccharides (EPS) via glucosyltransferases, and form retentive biofilms. The treatment caries with conventional antibiotics often ineffective due bacterium’s capacity recalcitrant To address these challenges, strategies specifically target pathogen’s virulence without affecting its viability have emerged as promising alternatives. In this context, we investigated anticariogenic properties methanolic extract Padina boergesenii (MEPB). MEPB demonstrated substantial, dose-dependent antibiofilm activity, maximum inhibition 93% at 128 μg/mL, compromising S. . Anti-virulence assays using sub-MIC (minimum inhibitory concentration) levels showed significant reductions factors: 75% reduction sucrose-dependent adherence, 65% sucrose-independent along notable decreases acid production, tolerance, water-insoluble (85%) water-soluble (52%) glucan synthesis. Additionally, significantly reduced cell surface hydrophobicity (55%) extracellular DNA (eDNA) production (64%). qPCR analysis corroborated vitro findings, revealing suppresses expression genes involved virulence, particularly related EPS synthesis ( gtfB , gtfC & gtfD ) biofilm formation( gbpB gbpC two-component regulatory system vicR) were downregulated. Toxicity testing on human buccal epithelial cells confirmed non-toxic nature MEPB, suggesting safety for potential therapeutic use. Furthermore, GC-MS/MS identified palmitic acid, myristic stearic major active constituents extract. Subsequent potent efficacy compounds: (85%), (72%) (83%). conclusion, study identifies P. biomolecules agents, offering an alternative approach combat by targeting bacterial mechanisms rather than viability.

Language: Английский

Functional Study of desKR: a Lineage-Specific Two-Component System Positively Regulating Staphylococcus aureus Biofilm Formation DOI Creative Commons
Xinyan Ma, Ziyan Wu,

Junpeng Li

et al.

Infection and Drug Resistance, Journal Year: 2024, Volume and Issue: Volume 17, P. 4037 - 4053

Published: Sept. 1, 2024

Biofilms significantly contribute to the persistence and antibiotic resistance of

Language: Английский

Citations

0
Anticariogenic activity of marine brown algae Padina boergesenii and its active components towards Streptococcus mutans DOI Creative Commons

Ravichellam Sangavi,

Nambiraman Malligarjunan,

Lakkakula Satish

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Nov. 25, 2024

Streptococcus mutans is a well-recognized bacterium that plays predominant role in the progression of dental caries. Its pathogenicity linked to several key characteristics, including ability produce organic acids (acidogenicity), thrive low pH environments (aciduricity), synthesize exopolysaccharides (EPS) via glucosyltransferases, and form retentive biofilms. The treatment caries with conventional antibiotics often ineffective due bacterium’s capacity recalcitrant To address these challenges, strategies specifically target pathogen’s virulence without affecting its viability have emerged as promising alternatives. In this context, we investigated anticariogenic properties methanolic extract Padina boergesenii (MEPB). MEPB demonstrated substantial, dose-dependent antibiofilm activity, maximum inhibition 93% at 128 μg/mL, compromising S. . Anti-virulence assays using sub-MIC (minimum inhibitory concentration) levels showed significant reductions factors: 75% reduction sucrose-dependent adherence, 65% sucrose-independent along notable decreases acid production, tolerance, water-insoluble (85%) water-soluble (52%) glucan synthesis. Additionally, significantly reduced cell surface hydrophobicity (55%) extracellular DNA (eDNA) production (64%). qPCR analysis corroborated vitro findings, revealing suppresses expression genes involved virulence, particularly related EPS synthesis ( gtfB , gtfC & gtfD ) biofilm formation( gbpB gbpC two-component regulatory system vicR) were downregulated. Toxicity testing on human buccal epithelial cells confirmed non-toxic nature MEPB, suggesting safety for potential therapeutic use. Furthermore, GC-MS/MS identified palmitic acid, myristic stearic major active constituents extract. Subsequent potent efficacy compounds: (85%), (72%) (83%). conclusion, study identifies P. biomolecules agents, offering an alternative approach combat by targeting bacterial mechanisms rather than viability.

Language: Английский

Citations

0