Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 1, 2024
Current Microbiology, Journal Year: 2025, Volume and Issue: 82(2)
Published: Jan. 13, 2025
Language: Английский
Citations
0
Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 324 - 324
Published: Feb. 2, 2025
Marine bacteria that have adapted to thrive in extreme environments, such as Pseudoalteromonas haloplanktis TAC125 (PhTAC125), offer a unique biotechnological potential. The discovery of an endogenous megaplasmid (pMEGA) raises questions about its metabolic impact and functional role strain. This study aimed at streamlining the host genetic background by curing PhTAC125 pMEGA plasmid using sequential approach. We combined homologous recombination exploiting suicide vector, with PTasRNA gene-silencing technology interfering replication machinery. approach led construction novel KrPL2 strain, cured plasmid, which exhibited no significant differences growth behavior, though showcasing enhanced resistance oxidative stress reduced capacity for biofilm formation. These findings represent achievement developing our understanding applications recombinant protein production. opens up possibility valuable elements further advancing tools PhTAC125.
Language: Английский
Citations
0
Life, Journal Year: 2025, Volume and Issue: 15(3), P. 354 - 354
Published: Feb. 24, 2025
In an investigation exploring endophytic microbiota from agricultural crops, aerobic, non-motile, Gram-negative, coccobacillus-shaped bacterial isolate, designated as strain NGMCC 1.201697T, was isolated maize roots in Hunan Province, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that 1.201697T belonged to the genus Paracoccus, showing highest sequence similarity Paracoccus broussonetiae CPCC 101403T (99.86%). The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) were 98.57% 87.90% between novel isolate its closest phylogenetic relative. However, phenotypic characterization further differentiated P. 101403T. showed enhanced environmental tolerance adaptability (growth 0–8% NaCl 4–37 °C), unique enzymatic activities (esterase C4, β-glucosidase, L-proline arylamidase, β-galactosidase), expanded metabolic capabilities (D-mannitol, D-cellobiose, saccharose, so on). major polar lipids consisted of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylglycerol (PG), two unidentified glycolipids (GLs) four phospholipids (PLs). predominant respiratory quinone ubiquinone-10, fatty acid summed feature 8 (C18:1 ω7c, 69.42%). DNA G + C content 64.49 mol%. Based results these analyses, represents a subspecies broussonetiae, for which name subsp. drimophilus nov. is proposed. type-strain (=CGMCC 1.61958T =JCM 37104T).
Language: Английский
Citations
0
PROTOPLASMA, Journal Year: 2025, Volume and Issue: unknown
Published: April 3, 2025
Language: Английский
Citations
0
Genetic Resources and Crop Evolution, Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 28, 2024
Language: Английский
Citations
2
Climate, Journal Year: 2024, Volume and Issue: 13(1), P. 1 - 1
Published: Dec. 24, 2024
Climate change is a global challenge that impacts rangeland and pastureland landscapes by inducing shifts in temperature variability, precipitation patterns, extreme weather events. These changes alter soil plant conditions, reducing forage availability chemical composition leading to nutritional stress cattle. This occurs when animals lack adequate water feed sources or these resources are insufficient quantity, composition, nutrient balance. Several strategies essential address impacts. Genetic selection, epigenetic biomarkers, exploration of memories present promising avenues for enhancing the resilience cattle populations improving adaptation environmental stresses. Remote sensing GIS technologies assist locating wet spots establish islands diversity high quality grazing amid ongoing climate challenges. Establishing functional improves quality, reduces carbon nitrogen footprints, provides nutrients bioactives, thus health, welfare, productivity. Real-time GPS collars coupled with accelerometers provide detailed data on movement activity, aiding livestock nutrition management while mitigating heat stress. Integrating may offer significant advantages facing changing world securing future production food system.
Language: Английский
Citations
1
Microbiology and Biotechnology Letters, Journal Year: 2024, Volume and Issue: 52(3), P. 233 - 254
Published: Sept. 16, 2024
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 13, 2024
Abstract Marine bacteria that have adapted to thrive in extreme environments, such as Pseudoalteromonas haloplanktis TAC125 ( Ph TAC125), offer a unique biotechnological potential. The discovery of an endogenous megaplasmid (pMEGA) raised questions about its metabolic impact and functional role this strain. This study aimed at streamlining the host genetic background by curing from pMEGA plasmid using sequential approach. We combined homologous recombination exploiting suicide vector with PTasRNA gene silencing technology interfere replication machinery. approach led construction novel KrPL 2 strain, cured plasmid, which exhibited no significant differences growth behaviour, though showcasing enhanced resistance oxidative stress reduced capability biofilm formation. These findings represent achievement for understanding applications recombinant protein production. opens up possibility exploit valuable elements further advancing tools TAC125.
Language: Английский
Citations
0
Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 1, 2024
Citations
0