Engineering a Cross-Feeding Synthetic Bacterial Consortium for Degrading Mixed PET and Nylon Monomers DOI Open Access

Ida Putu Wiweka Dharmasiddhi,

jinjin chen, Bahareh Sadat Arab

et al.

Processes, Journal Year: 2025, Volume and Issue: 13(2), P. 375 - 375

Published: Jan. 30, 2025

Plastics are indispensable to modern life, but their widespread use has created an environmental crisis due inefficient waste management. Mixed plastic waste, comprising diverse polymers, presents significant recycling challenges the high costs of sorting and processing, leading ecosystem accumulation harmful by-product generation. This study addresses this issue by engineering a synthetic bacterial consortium (SBC) designed degrade mixed monomers. The pairs Escherichia coli Nissle 1917, which uses ethylene glycol (EG), monomer derived from polyethylene terephthalate (PET), as carbon source, with Pseudomonas putida KT2440, metabolizes hexamethylenediamine (HD), nylon-6,6, nitrogen source. Adaptive evolution SBC revealed novel metabolic interaction where P. developed ability both EG HD, while E. played critical role in degrading glycolate, mitigating its toxicity. evolved cross-feeding pattern enhanced biomass production, efficiency, community stability compared monocultures. consortium’s performance was validated through flux balance analysis (FBA), high-performance liquid chromatography (HPLC), growth assays. These findings highlight potential SBCs addressing complex offering promising avenue for sustainable bioremediation advancing future polymer degradation strategies.

Language: Английский

A novel Gordonia sp. PS3 isolated from the gut of Galleria mellonella larvae: mechanism of polystyrene biodegradation and environmental toxicological evaluation DOI

Luhui Xu,

Xuejiao An,

Huoyong Jiang

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: unknown, P. 137219 - 137219

Published: Jan. 1, 2025

Language: Английский

Citations

1
Engineering a Cross-Feeding Synthetic Bacterial Consortium for Degrading Mixed PET and Nylon Monomers DOI Open Access

Ida Putu Wiweka Dharmasiddhi,

jinjin chen, Bahareh Sadat Arab

et al.

Processes, Journal Year: 2025, Volume and Issue: 13(2), P. 375 - 375

Published: Jan. 30, 2025

Plastics are indispensable to modern life, but their widespread use has created an environmental crisis due inefficient waste management. Mixed plastic waste, comprising diverse polymers, presents significant recycling challenges the high costs of sorting and processing, leading ecosystem accumulation harmful by-product generation. This study addresses this issue by engineering a synthetic bacterial consortium (SBC) designed degrade mixed monomers. The pairs Escherichia coli Nissle 1917, which uses ethylene glycol (EG), monomer derived from polyethylene terephthalate (PET), as carbon source, with Pseudomonas putida KT2440, metabolizes hexamethylenediamine (HD), nylon-6,6, nitrogen source. Adaptive evolution SBC revealed novel metabolic interaction where P. developed ability both EG HD, while E. played critical role in degrading glycolate, mitigating its toxicity. evolved cross-feeding pattern enhanced biomass production, efficiency, community stability compared monocultures. consortium’s performance was validated through flux balance analysis (FBA), high-performance liquid chromatography (HPLC), growth assays. These findings highlight potential SBCs addressing complex offering promising avenue for sustainable bioremediation advancing future polymer degradation strategies.

Language: Английский

Citations

0