The Combined Effect of Two Alternaria Mycotoxins (Alternariol and Alternariol Monomethyl Ether) on Porcine Epithelial Intestinal Cells DOI Creative Commons
Daniela Eliza Marin, Iulian Alexandru Grosu, Gina Cecilia Pistol

et al.

Agriculture, Journal Year: 2024, Volume and Issue: 14(9), P. 1478 - 1478

Published: Aug. 30, 2024

Alternariol (AOH) and alternariol monomethyl ether (AME) are secondary metabolites produced by fungi belonging to the genus Alternaria, which generally contaminate fruits but also cereal crops vegetables. The objective of this study was investigate if co-exposure swine epithelial intestinal cell line (IPEC-1) a mixture mycotoxins would cause an increase in toxicity as compared with exposure single toxin. effects individual toxins well those their combination (1:1 ratio), range 1–250 μM, were assessed vitro for viability proliferating IPEC-1 cells then on parameters related oxidative stress. Our results indicate that both AOH AME significantly decreased viability, cytotoxicity induced + higher than toxins. main interaction type ranged from slight synergy AOH-AME affecting 25% (CI = 0.88), evolved into synergistic effect level IL50 0.41) strong at IL90 0.10). In addition, we investigated two low concentrations (2.5 μM 5 μM) administered individually or stress cells. Both can induce reactive oxygen species—ROS (+) cells%—and damage porcine At least additive observed when exposed AOH-AME, consisting percentage ROS oxidation lipids, proteins, DNA toxin effect. A breakdown antioxidant defense after toxins, decrease activity enzymes superoxide dismutase (SOD) catalase (CAT), no synergic resulted conclusion, our data interfere proliferation Moreover, had dose-dependent viability. Also, stronger However, signaling pathways responsible AOH, AME, combinations need further investigations order provide important risk assessments case contamination feed Alternaria

Language: Английский

Qualitative and relative abundance analysis of Alternaria alternata metabolites in pomegranate (Punica granatum L.) cultivars affected by Heart Rot using UHPLC-Q-TOF-MS DOI Creative Commons
Mario Riolo,

Luz del C.,

Bua C.

et al.

Food Control, Journal Year: 2025, Volume and Issue: unknown, P. 111204 - 111204

Published: Feb. 1, 2025

Language: Английский

Citations

0
Toxin Production by Alternaria alternata in Black Spot Disease of Chrysanthemum morifolium ‘Fubai’: Accumulation of Altenuene and Tenuazonic Acid in Flowers DOI Creative Commons
Qingling Zhan, Lina Liu, Wenjie Li

et al.

Toxins, Journal Year: 2025, Volume and Issue: 17(4), P. 181 - 181

Published: April 5, 2025

Alternaria species produce diverse secondary metabolites that act as critical virulence factors during plant pathogenesis. In cultivation areas of Chrysanthemum morifolium ‘Fubai’—a key cultivar for herbal tea—black spot disease caused by A. alternata manifests necrotic leaf lesions progressing to wilting. Despite this disease’s economic impact, information on its associated toxins is limited, and the types produced black pathogen ‘Fubai’ in particular remain unclear. Furthermore, whether are present flowers when leaves show symptoms uncertain, but their inflorescence not visibly infected. Using two previously characterized strains (F16/F20) isolated from earlier studies, we demonstrated concomitant production altenuene (ALT) tenuazonic acid (TeA) both strains, with strain-specific yield variations (F20 TeA: 342.16 µg/mL vs. F16: 21.84 µg/mL; ALT: 0.28 0.90 µg/mL). Time-course monitoring revealed coordinated accumulation inoculated petals, reaching 18.07 μg/g ALT 2.59 µg/g TeA day 9. Notably, these were detected only flower samples spot-infected plants, concentrations correlated closely severity leaves. Moreover, although inflorescences did display symptoms, fresh dried retained detectable toxin levels. We established a technical system extraction quantitative detection tea chrysanthemum. This work provides first confirmation ALT/TeA co-contamination ‘Fubai’, revealing substantial dietary exposure risks through consumption. Our findings suggest that, food safety risk reduction perspective, integrated management strategies should be developed minimize contamination chrysanthemum, including improved prevention measures potential regulatory considerations.

Language: Английский

Citations

0
Absolute oral bioavailability, quantitative toxicokinetics and metabolite profiling of alternariol and alternariol monomethyl ether in pigs DOI Creative Commons

Danica den Hollander,

Siegrid De Baere,

Celestien Holvoet

et al.

Archives of Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: April 26, 2025

Abstract The mycotoxins alternariol (AOH) and monomethyl ether (AME), produced by Alternaria spp . , are common contaminants of food feed a potential threat to animal human health. To date, the most prominent data gaps for their comprehensive risk assessment concern information on in vivo absorption, distribution, metabolism excretion (ADME) toxicokinetic behavior. aim this study was determine absolute oral bioavailability, quantitative characteristics biotransformation AOH AME pigs, using crossover trials with intravenous administration single dose both at 2 mg/kg b.w. Plasma profiles phase I II metabolites were studied vena jugularis UPLC-MS/MS LC-HRMS methods. Furthermore, plasma from portae analyzed presystemic biotransformation. Urine collected urinary metabolite profiles. Results reveal low bioavailability (15%) (9%), caused absorption and/or extensive first-pass liver mainly II, lesser extent metabolites. Quantitative modeling IV showed high total body clearance (12.9 16.8 L/(h*kg b.w.), respectively), volume distribution (4.97 5.15 L/kg b.w., respectively) short elimination half-life 0.16 0.21 h, respectively. These findings may contribute development candidate biomarkers exposure biomonitoring studies since pigs considered suitable model extrapolate humans.

Language: Английский

Citations

0
Integrative multi-omics analysis of the microbiome and metabolome in bronchoalveolar lavage fluid from patients with early-stage lung cancer DOI Creative Commons
Jiajun Xie,

Nengyang Zhu,

Weiguo Xu

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: April 28, 2025

Lung cancer is a significant health concern that poses considerable threat to human and quality of life. In order enhance the prognosis patients with lung cancer, we conducted combined analysis 16S rDNA gene sequencing alveolar lavage fluid LC-MS metabolomics research, objective identifying biomarkers in early-stage presenting as SPN. A comparison benign nodule group revealed phylum-level Bacteroidetes genus-level Chryseobacterium Delftia were more abundant latter group. Additionally, Fusobacteriales might serve predictive marker for diagnosis cancer. context metabolomics, was found be characterised by elevated levels specific metabolites, including Alternariol, dTMP, Oxymatrine, Gedunin, PC 36:4. Conversely, reductions other such LPC O-24:0, 18:2_18:3, 19:2_19:2, Cholecalciferol T-2 Triol, also observed. Correlation analyses demonstrated microorganisms closely associated differential metabolites. Specifically, variety high-abundance flora involved vitamin digestion absorption pathways. Furthermore, cholecalciferol may robust predictor These findings provide new manifested SPN, which clinically important requires further study potential mechanisms action function targets.

Language: Английский

Citations

0
An overview of cyclodextrin on the detection and removal of mycotoxin: recent advances and future prospects DOI
Yan Shi, Wei Xu, Wenli Zhang

et al.

Food Control, Journal Year: 2024, Volume and Issue: 165, P. 110660 - 110660

Published: June 17, 2024

Language: Английский

Citations

1
Brain health: Pathway to primary prevention of neurodegenerative disorders of environmental origin DOI
Peter S. Spencer, Shala Ghaderi Berntsson, Alain Buguet

et al.

Journal of the Neurological Sciences, Journal Year: 2024, Volume and Issue: 468, P. 123340 - 123340

Published: Dec. 9, 2024

Language: Английский

Citations

1
The Combined Effect of Two Alternaria Mycotoxins (Alternariol and Alternariol Monomethyl Ether) on Porcine Epithelial Intestinal Cells DOI Creative Commons
Daniela Eliza Marin, Iulian Alexandru Grosu, Gina Cecilia Pistol

et al.

Agriculture, Journal Year: 2024, Volume and Issue: 14(9), P. 1478 - 1478

Published: Aug. 30, 2024

Alternariol (AOH) and alternariol monomethyl ether (AME) are secondary metabolites produced by fungi belonging to the genus Alternaria, which generally contaminate fruits but also cereal crops vegetables. The objective of this study was investigate if co-exposure swine epithelial intestinal cell line (IPEC-1) a mixture mycotoxins would cause an increase in toxicity as compared with exposure single toxin. effects individual toxins well those their combination (1:1 ratio), range 1–250 μM, were assessed vitro for viability proliferating IPEC-1 cells then on parameters related oxidative stress. Our results indicate that both AOH AME significantly decreased viability, cytotoxicity induced + higher than toxins. main interaction type ranged from slight synergy AOH-AME affecting 25% (CI = 0.88), evolved into synergistic effect level IL50 0.41) strong at IL90 0.10). In addition, we investigated two low concentrations (2.5 μM 5 μM) administered individually or stress cells. Both can induce reactive oxygen species—ROS (+) cells%—and damage porcine At least additive observed when exposed AOH-AME, consisting percentage ROS oxidation lipids, proteins, DNA toxin effect. A breakdown antioxidant defense after toxins, decrease activity enzymes superoxide dismutase (SOD) catalase (CAT), no synergic resulted conclusion, our data interfere proliferation Moreover, had dose-dependent viability. Also, stronger However, signaling pathways responsible AOH, AME, combinations need further investigations order provide important risk assessments case contamination feed Alternaria

Language: Английский

Citations

0