Synergistic Anti-Inflammatory Effects of Pomegranate Peel–Hawthorn Combinations in Ulcerative Colitis: Network Pharmacology Prediction and Experimental Validation

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(4), P. 243 - 243

Published: April 1, 2025

Ulcerative colitis (UC) is a chronic inflammatory bowel disease characterized by complex pathogenesis involving dysregulated immunity and gut microbiota imbalance, demanding innovative therapeutic strategies. This study investigates the synergistic potential of pomegranate peel–hawthorn combinations their active constituents (ellagic acid maslinic acid) through an integrative approach combining network pharmacology, in vitro/in vivo experiments, analysis. Network pharmacology identified 61 shared targets (p < 0.05 for pathway enrichment) revealed complementary mechanisms: peel primarily modulated AGE-RAGE/PI3K-Akt pathways, while hawthorn targeted IL-17/NF-κB signaling. Experimental validation demonstrated potent anti-inflammatory effects (combination index 1), with optimal reducing nitric oxide production 52.35% (herbal extracts, p 0.05) 74.4% (active monomers, 0.05). In DSS-induced UC mice, combinatorial therapies significantly suppressed pro-inflammatory cytokines (TNF-α: 204.78 vs. 446.52 pg/mL group, 0.05; IL-6: 33.19 64.86 pg/mL, 0.05), restored colonic SOD activity (72.31 50.10 U/mg·prot 0.01), alleviated histopathological damage, outperforming monotherapeutics. Gut analysis recovery α-diversity indices normalized Bacteroidota/Bacillota ratios. Mechanistically, MAPK/NF-κB signaling cascades, p-p38/p38 0.01 group) p-ERK1/2/ERK1/2 phosphorylation. These findings establish that formulations exert multi-modal inhibition mitigation oxidative stress, restoration microbiota, offering scientifically validated management rooted traditional medicine principles.

Language: Английский

Metabolomics reveals the metabolic disturbance caused by arsenic in the mouse model of inflammatory bowel disease

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 287, P. 117305 - 117305

Published: Nov. 1, 2024

Arsenic exposure has long been a significant global health concern due to its association with various human diseases. The adverse effects of arsenic can be influenced by multiple factors, resulting in considerable individual variability. Individuals inflammatory bowel disease (IBD) are particularly vulnerable the toxin exposure, yet specific impact context IBD remains unclear. In this study, we employed non-targeted metabolomics approach investigate how affects metabolic homeostasis an model using Helicobacter trogontum-infected interleukin-10 deficient mice. Our results demonstrated that disrupted balance metabolites, including tryptophan, polyunsaturated fatty acids, purine and pyrimidine branched-chain amino mice colitis but not those without colitis. Notably, several crucial metabolites involved anti-inflammatory responses, oxidative stress, energy metabolism were significantly altered These indicate lead extensive disturbances, potentially exacerbating severity impacting overall health. This study underscores necessity evaluating toxicity relation better understand mitigate associated risks.

Language: Английский