Yellow mealworm beetle (Tenebrio molitor) Larvae as an Alternative Model for Antileishmanial Drug Evaluation

Veterinary Parasitology, Journal Year: 2025, Volume and Issue: 337, P. 110468 - 110468

Published: April 17, 2025

Language: Английский

Arylacetamides exhibit antiproliferative effects on non-transformed mammalian and vegetal cells and toxicity on crustaceans and fish embryos

Drug and Chemical Toxicology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 11

Published: May 8, 2025

Non-clinical steps for development, validation and biosafety of new medicines products comprises studies on cells, proteins, animals. Herein, we evaluated the toxic activity antitumoral 2-chloro-N-arylacetamides eukaryotic dividing cells animal replacement models. Firstly, cytotoxicity chloro (compound 2), bromo 3) nitro 4) acetamides was analyzed by fluorescent assays in fibroblasts. Next, toxicity Allium cepa meristematic 48h-living Artemia salina larvae. Finally, embryos Danio rerio (zebrafish) were exposed to compound 2 (0.14 - 7.2 μg/mL) 120 h exposure. All arylacetamides cytotoxic murine human fibroblasts, with IC50 values ranging from 1.2 μg/mL (5.6 µM = 4 L-929) 4.9 (24 MRC-5 cells), respectively, inhibited root growth 10 100 µg/mL, corroborated mitotic index reduction cell cycle arrest interphase (p < 0.05) without clastogenic injuries. Compound showed time- concentration-dependent killing effects zebrafish embryos. Its 24 h-acute at higher concentrations (1.93 90% 100% death) aquatic A. organisms. After 96 exposure 0.52 (2.55 µM), almost more than one lethal/sublethal morphological abnormality 0.05). Then, all unspecific effects, mainly halogenated electrophile chloroacetamide. They present strong antimitotic action vertebrate vegetal although such antiproliferative does not seem be directly related chromosomal damage inductions.

Language: Английский

Zebrafish as a Model Organism for Congenital Hydrocephalus: Characteristics and Insights

Zebrafish, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 7, 2024

Hydrocephalus is a cerebrospinal fluid-related disease that usually manifests as abnormal dilation of the ventricles, with triad clinical findings including walking difficulty, reduced attention span, and urinary frequency or incontinence. The onset congenital hydrocephalus closely related to mutations in genes regulate brain development. Currently, our understanding mechanisms remains limited, prognosis existing treatments unsatisfactory. Additionally, there are no suitable dedicated model organisms for hydrocephalus. Therefore, it significant determine mechanism develop special animal models Recently, zebrafish have emerged popular organism many fields, developmental biology, genetics, toxicology. Its genome shares high similarity humans, has fast low-cost reproduction. These advantages make studying pathogenesis therapeutic approaches various diseases, specifically diseases. This study explored possibility using review describes characteristics discusses specific models. limitations research highlighted, insights further development provided.

Language: Английский