Poly-γ-glutamic acid alleviates slow transit constipation by regulating aquaporin and gut microbes

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 10, 2025

Slow transit constipation (STC) is a prevalent gastrointestinal disorder caused by colon dysfunction. Poly-γ-glutamic acid (γ-PGA), an anionic polymer known for its moisture retention, degradability, and food safety, was studied effects on loperamide-induced STC in mice. Treatment with γ-PGA one week significantly increased both defecation frequency fecal water content, the high-dose group (10 g/kg/d) restoring content to 34.23%, outperforming low- (16.16%) medium-dose (27.08%) groups exceeding positive control, PEG, 1.35 times. enhanced intestinal peristalsis reduced expression of inflammatory markers (IL-1β, IL-6, caspase-1, TLR2) water-electrolyte transport genes (AQP3, AQP4, ENaC-β), while improving tight junction proteins (Claudin-1, Occludin, ZO-1) damaged loperamide. Histopathological analyses confirmed γ-PGA's capacity repair damage. Additionally, Western Blot analysis indicated AQP3/4 levels colon, molecular docking showed good binding affinity between AQPs. also positively altered gut microbiota composition. Overall, shows promise treating modulating aquaporins microbiota.

Language: Английский

Tangeretin Improves the Memory of Swiss Mice, Suggesting Potential Molecular Interventions Through Animal Behavior Assessments and In Silico Studies

Brain and Behavior, Journal Year: 2025, Volume and Issue: 15(5)

Published: May 1, 2025

ABSTRACT Introduction Tangeretin (TAN), a polymethoxylated flavone from citrus peels, exhibits neuroprotective, anti‐inflammatory, and antioxidant properties. This study aims to evaluate the memory‐enhancing effects of TAN in Swiss mice explore its potential molecular interactions with D 2 dopamine (DOP) receptor through vivo behavioral assessments silico approaches. Methods were administered (10 20 mg/kg), DOP (22 olanzapine (OLN) (2 alone combinations per orally (p.o.), followed by cognitive using marble burying, dust removal, trained swimming tests. In studies included docking against (PDB: 6CM4), pharmacokinetics (SwissADME, pkCSM), toxicity predictions (ProTox‐3). Results significantly ( p < 0.05) improved functions, including memory, anxiety, motor coordination, dose‐dependent manner, mg/kg showing most notable effect. The combination TAN‐10 DOP‐22 enhanced these benefits, whereas OLN‐2 reduced improvements. TAN‐treated showed better performance tests, indicating problem‐solving, coordination. These results suggest TAN's enhancement, particularly DOP‐22. No deaths observed any treatment group, all treated animals exhibited normal physiological activity no signs acute toxicity. revealed that strongest binding affinity (BA) (−6.6 kcal/mol) receptor, forming multiple hydrogen bonds (HBs), which indicates mechanism for memory enhancement via dopaminergic modulation. Pharmacokinetic analyses also has favorable ADMET properties, high gastrointestinal absorption, blood–brain barrier penetration, low Conclusion findings highlight as promising therapeutic candidate memory‐related disorders, warranting further clinical exploration.

Language: Английский

Fruit-derived bioactive compounds of Malus domestica as novel therapeutic inhibitors against Monkeypox and Marburg virus: A computational based drug discovery study

Food Chemistry Advances, Journal Year: 2025, Volume and Issue: 7, P. 100998 - 100998

Published: May 10, 2025

Language: Английский