Herein,
a
novel
functional
DNA
-linked
immunosorbent
assay
(DLISA)
with
dual-modality
based
on
hybrid
chain
reaction
(HCR)
has
been
successfully
developed
for
ultrasensitive
detection
of
aflatoxin
B1
(AFB1).
The
strategy
relies
AFB1
immune-bridged
occurrence
HCR
accompanying
the
formation
double-stranded
polymer
and
salt-induced
aggregation
gold
nanoparticles
(AuNPs).
Concretely,
an
aptamer-initiator
stand
(Apt-Ini
stand)
is
designed
recognition
activation
HCR,
which
anchored
to
96-well
plates
by
antibody.
This
Apt-Ini
can
recognize
matched
hairpins
cause
crossing-opening
H1
H2,
triggering
producing
long
polymer.
After
addition
SYBR
Green
I,
fluorescent
signal
was
achieved.
remaining
were
added
stuck
surface
AuNPs,
protecting
AuNPs
against
aggregation.
A
colorimetric
response
from
red
blue
caused
provides
limits
detections
1.333×10-14
g/mL
2.471×10-15
colorimetric/fluorescence
signals,
respectively.
not
only
colorimetry
that
meet
needs
on-the-spot
preliminary
inspection,
but
also
fluorescence
acquire
precise
results
in
laboratory.
Environmental Technology & Innovation,
Journal Year:
2024,
Volume and Issue:
34, P. 103625 - 103625
Published: April 4, 2024
Contaminants,
such
as
nucleic
acids
or
toxic
small
molecules,
threaten
both
human
health
and
ecosystems
when
they
infiltrate
the
environment.
The
precise
highly
sensitive
identification
of
contaminants
holds
paramount
importance
across
diverse
domains,
including
safeguarding
food
integrity,
facilitating
clinical
diagnostics,
monitoring
environmental
conditions.
Traditional
methodologies,
encompassing
spectroscopy,
chromatography,
sequencing,
metagenomics,
have
conventionally
served
pivotal
roles
in
detection
processes.
Nevertheless,
these
methods
encountered
recurring
challenges
related
to
sensitivity,
specificity,
portability.
This
review
focuses
on
groundbreaking
CRISPR/Cas12-based
biosensors.
These
biosensors
leverage
incredible
precision
programmability
CRISPR/Cas
system
recognize
specific
targets.
Here,
we
comprehensively
assess
fundamental
mechanisms
that
enable
detection,
ranging
from
guide
RNA
design
collateral
cleavage.
versatility
CRISPR/Cas12
becomes
evident
through
their
applications.
applications
encompass
medical
safety,
monitoring.
transition
conventional
ultimately
represents
a
significant
milestone
contaminant
detection.
By
incorporating
molecular
biology,
nanotechnology,
bioinformatics,
potential
reshape
landscape
water
CRIPSR-Cas
diagnostics
is
transformative
technology
paves
way
for
safer
healthier
future
environment
life.
Frontiers in Microbiology,
Journal Year:
2024,
Volume and Issue:
15
Published: Feb. 6, 2024
Major
health
events
caused
by
pathogenic
microorganisms
are
increasing,
seriously
jeopardizing
human
lives.
Currently
PCR
and
ITA
widely
used
for
rapid
testing
in
food,
medicine,
industry
agriculture.
However,
due
to
the
non-specificity
of
amplification
process,
researchers
have
proposed
combination
nucleic
acid
technology
with
novel
CRISPR
detection,
which
improves
specificity
credibility
results.
This
paper
summarizes
research
progress
conjunction
CRISPR/Cas
detection
pathogens,
provides
a
reference
theoretical
basis
subsequent
application
field
pathogen
detection.
ACS Omega,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 26, 2025
Molecular
logic
gates,
as
biomolecule-based
computational
systems,
are
highly
suitable
for
multitarget
detection
due
to
their
programmability
and
modularity.
However,
existing
systems
primarily
limited
nucleic
acid
have
not
been
widely
applied
disease-related
sensing,
particularly
disease
antigens.
CD33
CD123
critical
biomarkers
acute
myeloid
leukemia
(AML),
yet
conventional
methods
rely
on
expensive
equipment
complex
procedures,
limiting
accessibility
practicality.
This
study
designs
a
DNA
gate
system
integrating
aptamers,
catalytic
hairpin
assembly
(CHA),
CRISPR-Cas12a,
pioneering
its
use
AML
antigen
detection.
The
comprises
three
modules:
input
recognition,
signal
amplification,
transduction.
Nucleic
aptamers
specifically
identify
CD123,
while
CHA
enables
efficient
amplification
CRISPR-Cas12a
generates
fluorescent
output
via
trans-cleavage
activity.
operates
stably
at
room
temperature
implements
multiple
models,
including
YES,
OR,
AND,
NOR,
INHIBIT,
enabling
the
simultaneous
of
CD123.
Experimental
results
visually
distinguishable
under
blue
light,
requires
only
standard
fluorescence
instruments.
In
serum
samples,
it
exhibits
excellent
selectivity
stability,
with
limit
0.5
ng/mL.
pioneers
application
technology
detection,
addressing
gap
in
biomarker
sensing.
Our
indicates
that
this
platform,
characterized
by
simplicity
operation,
high
sensitivity,
versatility
functions,
holds
promise
potent
sensing
intelligent
multiplex
target
antigens,
environmental
pollutants,
heavy
metals.
