Quantitative Study on the Charge-Dependent Uptake of Ultrasmall Fluorescent Gold Nanoclusters in 3D Spheroids of Cancer Cells

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Gold nanoclusters (AuNCs) have garnered significant attention in biomedical applications, particularly biosensing, cancer therapy, and imaging, due to their unique optical property, good biocompatibility, distinct bioactivity. Understanding the cellular uptake behavior of AuNCs is critical improve efficacy whose mechanism has not been adequately validated. In this work, we synthesized with varying surface modifications quantify exact law charge on a multidimensional manner by using 3D multicellular tumor spheroids both HeLa cells MCF-7 as model system. By combined use fluorescence live cell imaging inductively coupled plasma–mass spectrometry, systematically investigated effect rate, intracellular versus intercellular distribution, penetration depth quantitative manner. Our results showed that was strongly dependent, efficiency increasing degree positive charges. A similar charge-dependent observed 2D cultures spheroids, but difference less pronounced, comparison model. The AuNCs' quantified multiple dimensions which also provides crucial knowledge for effective therapeutics applications based other nanomaterials.

Language: Английский

Molecular Packing-Driven Manipulation of Aggregation Induced Emission in Gold Nanoclusters

The Journal of Physical Chemistry Letters, Journal Year: 2024, Volume and Issue: 15(33), P. 8510 - 8519

Published: Aug. 12, 2024

A key limitation of supramolecular force-driven molecular assembly in aggregation-induced emission (AIE) materials is the need to precisely regulate interactions within assembly. Achieving such assemblies with situ manipulable arrangements could provide valuable insights into role forces AIE. Herein, by using glutathione-protected gold nanoclusters (AuNCs) as a model AIE material and naturally occurring polyphenol, tannic acid (TA), assembling agent, we demonstrate that dominated covalent bonds hydrogen bonding show enhanced AIE, while those π-π stacking promote charge transfer, resulting significant photoluminescence (PL) quenching. This phenomenon primarily stems from oxidation TA smaller aromatic ring structures, leading an increase interactions. The complete induces morphological transition 3-D spherical 2-D sheet-like structures due dominance interactions, consequently PL quenching AuNCs. These findings highlight critical packing modulating properties

Language: Английский

Tailoring the Photoluminescence of AIE-Type Gold Nanoclusters via Biomineralization-Inspired Polymorphism

Nanoscale, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

By mimicking biomineralization, this study introduces a facile approach to incorporate Au 22 SG 18 nanoclusters within different polymorphs of CaCO 3 and reveal that variations in the nanocluster photoluminescence are linked polymorph stability.

Language: Английский