Exploring the Interaction of Biotinylated FcGamma RI and IgG1 Monoclonal Antibodies on Streptavidin-Coated Plasmonic Sensor Chips for Label-Free VEGF Detection DOI Creative Commons
Soodeh Salimi Khaligh, Fahd Khalid-Salako, Hasan Kurt

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(12), P. 634 - 634

Published: Dec. 20, 2024

Vascular endothelial growth factor (VEGF) is a critical angiogenesis biomarker associated with various pathological conditions, including cancer. This study leverages pre-biotinylated FcγRI interactions IgG1-type monoclonal antibodies to develop sensitive VEGF detection method. Utilizing surface plasmon resonance (SPR) technology, we characterized the binding dynamics of immobilized biotinylated an antibody, Bevacizumab (AVT), through kinetic studies and investigated suitable conditions for sensor regeneration. Subsequently, FcγRI-captured AVT VEGF, calculating constants affinity. A calibration curve was established analyze quantification capacity accuracy biosensor, computing limits blank, detection, at 95% confidence interval. Additionally, specificity biosensor over other protein analytes assessed. innovative biomimetic approach enabled FcγRI-mediated site-specific capture, establishing stable reusable platform detecting accurately quantifying VEGF. The results indicate effectiveness plasmonic making it research applications and, potentially, clinical diagnostics. FcγRI-IgG1 antibody binding, this highlights industrial value advanced biosensing technologies, offering insights enhance therapeutic monitoring improve outcomes in anti-VEGF therapies.

Language: Английский

Advancing aflatoxin analytical techniques: harnessing next-generation technologies and industrial innovations for precision quantification and qualitative profiling—a prospective approach toward public health safeguarding DOI
Ishrat Perveen,

Muhammad Yaqoob,

Sajid Hameed

et al.

Journal of Food Measurement & Characterization, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

Language: Английский

Citations

0
A Review on Various Surface Plasmon Resonance-Based Sensors DOI

S. Rafi Ahamed,

Kishore Kumar Venkatesan,

Shuaib Abrar Jalaludeen

et al.

Plasmonics, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Language: Английский

Citations

0
Magnetic Fe3O4@Au@PDA core-shell nanoparticle-enhanced SPR detection of tebuconazole DOI
Bing Zhang, Yulian Li, Li Wang

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113472 - 113472

Published: March 1, 2025

Language: Английский

Citations

0
Progress in Surface Plasmon and Other Resonance Biosensors for Biomedical Applications DOI Creative Commons
Faten Bashar Kamal Eddin,

Houxin Fan,

Zhenchao Liu

et al.

Advanced Materials Technologies, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Abstract In today's technologically advanced era, the demand for swift and precise analyses spans scientific research industrial applications alike. Surface plasmon resonance (SPR) technology has surged in popularity, due to its real‐time measurement capabilities with exceptional sensitivity label‐free operation. These attributes have propelled SPR into prominence across diverse fields including medical diagnostics, pharmaceuticals, food safety, environmental monitoring of hazardous compounds. Here key principles behind are delved, workings SPR‐based sensors elucidated. Additionally, latest advancements biosensors tailored detecting a variety analytes, focus on dopamine (DA) α‐synuclein, which hold particular significance context neurological disorders, examined. potential breakthroughs discussed, thanks integration nanomaterials, lab‐on‐a‐chip devices, multiplexing formats, machine learning. Finally, new modes discussed based subwavelength structures leveraging guided mode (GMR), surface lattice (SLR), bound state continuum (BIC) their applications, particularly nonplasmonic refractive index biosensors.

Language: Английский

Citations

0
Numerical Investigation of a D‐Shaped Fiber‐Optic Biosensor Utilizing Surface Plasmon Resonance for Early Cancer Cell Detection DOI Open Access
Ahmed Akouibaa,

Abdelilah Akouibaa,

Rachid Masrour

et al.

Published: Aug. 7, 2024

The field of biosensors has found great use for surface plasmon resonance (SPR), which attracted attention its multiple applications. With the goal early cancer detection at level a single living cell, this research numerically investigates very sensitive D-shaped fiber-optic biosensor based on (SPR). titanium oxide (TiO2) is coated gold (Au), utilized as thin-film plasmonic material in suggested structure. guiding light fiber modeled using finite element method (FEM) when six different types healthy human cells and their malignant counterparts are exposed to region. Refractive index (RI) differences between through analysis optical spectra amplitudes. This enables us identify small changes characteristics that could be sign pathogenic alterations. In work, we concentrated three forms cancer: skin (basal cell) breast (MDA-MB-231 MCF-7). results show parameters optimal ensure sensor performs well used detect under study. When sensor’s performance compared SPR sensors have previously been published literature, it becomes clear our offers promise detection.

Language: Английский

Citations

0
Analytical and bioanalytical chemistry for digital diagnostics in digital healthcare DOI Creative Commons
Antje J. Baeumner

Analytical and Bioanalytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 31, 2024

Language: Английский

Citations

0
Exploring the Interaction of Biotinylated FcGamma RI and IgG1 Monoclonal Antibodies on Streptavidin-Coated Plasmonic Sensor Chips for Label-Free VEGF Detection DOI Creative Commons
Soodeh Salimi Khaligh, Fahd Khalid-Salako, Hasan Kurt

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(12), P. 634 - 634

Published: Dec. 20, 2024

Vascular endothelial growth factor (VEGF) is a critical angiogenesis biomarker associated with various pathological conditions, including cancer. This study leverages pre-biotinylated FcγRI interactions IgG1-type monoclonal antibodies to develop sensitive VEGF detection method. Utilizing surface plasmon resonance (SPR) technology, we characterized the binding dynamics of immobilized biotinylated an antibody, Bevacizumab (AVT), through kinetic studies and investigated suitable conditions for sensor regeneration. Subsequently, FcγRI-captured AVT VEGF, calculating constants affinity. A calibration curve was established analyze quantification capacity accuracy biosensor, computing limits blank, detection, at 95% confidence interval. Additionally, specificity biosensor over other protein analytes assessed. innovative biomimetic approach enabled FcγRI-mediated site-specific capture, establishing stable reusable platform detecting accurately quantifying VEGF. The results indicate effectiveness plasmonic making it research applications and, potentially, clinical diagnostics. FcγRI-IgG1 antibody binding, this highlights industrial value advanced biosensing technologies, offering insights enhance therapeutic monitoring improve outcomes in anti-VEGF therapies.

Language: Английский

Citations

0