Capillary electromigration methods for food analysis and Foodomics: Advances and applications in the period February 2019–February 2021

Electrophoresis, Journal Year: 2021, Volume and Issue: 43(1-2), P. 37 - 56

Published: Sept. 2, 2021

Abstract This work presents a revision of the main applications capillary electromigration methods in food analysis and Foodomics. Articles that were published during period February 2019‐February 2021 are included. The shows multiple CE have been developed applied to analyze different types molecules foods. Namely, amino acids, biogenic amines, carbohydrates, chiral compounds, contaminants, DNAs, additives, heterocyclic lipids, secondary metabolites, peptides, pesticides, phenols, pigments, polyphenols, proteins, residues, toxins, vitamins, small organic inorganic as well other minor compounds. last results on use for monitoring interactions processing, including recent microchips developments new Foodomics, discussed too. procedures investigate quality safety, nutritional value, storage bioactivity also included present review work.

Language: Английский

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Novel eDNA approaches to monitor Western honey bee (Apis mellifera L.) microbial and arthropod communities

Environmental DNA, Journal Year: 2023, Volume and Issue: 6(1)

Published: May 2, 2023

Abstract Pests and pathogens are a continuous threat to the health of Western honey bee Apis mellifera L. Monitoring colonies for arthropod pests, disease‐causing bacteria fungi, early detection new invasions is essential maintain pollination services provided by bees. We investigated feasibility using eDNA metabarcoding detect pests in hives across their foraging environment. sampled 13 sources within outside from our test apiary determine where most informative could be obtained, with thrice. This resulted 61 samples, 20 negative controls, three positive controls. Furthermore, we compared two collection techniques—wiping surfaces moistened forensic swabs spray/wash technique that aggregated surface into container, before collecting on filter. used DNA universal primer sets target arthropod, bacterial, fungal communities. Our results showed yielded sufficient swab methods were similar when they applied same surface. detected bacterial symbionts, mycotoxin‐producing fungi Brachymyrmex sp. rover ants. Common concern bees (i.e., [small hive beetle ( Aethina tumida ), Varroa destructor , Melissococcus plutonius ]) detected. matched visual observations clinical signs these tested. some species was source specific, which has implications as monitoring tool. Collectively, data show can accurately arthropods microbes contact comprehensive molecular predictor tool colony surveys.

Language: Английский

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Bee foraging preferences, microbiota and pathogens revealed by direct shotgun metagenomics of honey

Molecular Ecology Resources, Journal Year: 2022, Volume and Issue: 22(7), P. 2506 - 2523

Published: May 20, 2022

Honeybees (Apis mellifera) continue to succumb human and environmental pressures despite their crucial role in providing essential ecosystem services. Owing foraging honey production activities, honeybees form complex relationships with species across all domains, such as plants, viruses, bacteria other hive pests, making a valuable biomonitoring tool for assessing ecological niche. Thus, the application of shotgun metagenomics (SM) has paved way detailed description interact with. Nevertheless, SM bioinformatics tools DNA extraction methods rely on resources not necessarily optimized honey. In this study, we compared five widely used taxonomic classifiers using simulated communities commonly found We that Kraken 2 threshold 0.5 performs best distribution. also simple NaOH-based methodology (Direct-SM), which profiled seasonal variability similarly an established column-based approach (SM). Both approaches produce results consistent melissopalinology analysis describing botanical landscape surrounding apiary. Interestingly, detected strong stability constituting core noncore gut microbiome seasons, pointing potential utility noninvasive assessment bee microbiota. Finally, Direct-SM detect Varroa correlates well mite infestation observed hives. These observations suggest comprehensively describe honeybee niches can be tested building block large-scale studies assess health field.

Language: Английский

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Bacterial Profile and Fatty Acid Composition of Anatolian Bee Bread Samples by Metataxonomic and Metabolomic Approach

Current Microbiology, Journal Year: 2023, Volume and Issue: 80(3)

Published: Feb. 1, 2023

Language: Английский

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Chemical Analysis and in Vitro Antiviral Effects of Northeast Türkiye Propolis Samples against HSV‐1

Chemistry & Biodiversity, Journal Year: 2023, Volume and Issue: 20(8)

Published: June 21, 2023

Propolis is one of the mixtures with widest biological activity among natural products used in complementary medicine. HSV-1 a highly contagious and endemic virus. Available drugs are insufficient for recurrent infections. Therefore, new approaches to treat infections still being developed. In this study, it was aimed investigate inhibition effect ethanolic Anatolian propolis extracts obtained from Eastern Black Sea Region (Pazar, Ardahan, Uzungöl) on HSV-1. addition total phenolic (TPC) flavonoid content (TFC), profiles were analyzed by HPLC-UV. The antiviral tested 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), quantitative Real Time Polymerase Chain Reaction (qRT-PCR), plaque reduction tests, results evaluated statistically. It determined that amount substances varied between 44.12 166.91 mg GAE/g, samples 12.50 41.58 (mg QUE/g). shown all current study effective against HSV-1, but higher compounds contained showed activity. show promising candidates treatment.

Language: Английский

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