Investigating Hippocampal Proteome Dynamics in Aging Rats with Minimal Hepatic Encephalopathy via High-Resolution Mass Spectrometry DOI Creative Commons
Vishal Vikram Singh, Shambhu Kumar Prasad, Arup Acharjee

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Abstract The aging population faces a gradual decline in physical and mental capacities, with an increased risk of liver cirrhosis chronic diseases leading to hepatic encephalopathy (HE). intertwining physiological manifestations the pathophysiology HE significantly impairs cognitive ability, reduces quality life, increases mortality. Hence, effective therapeutic intervention is imperative. present study investigated impact minimal (MHE) on impairment rat by analyzing hippocampal proteome dynamics. For this purpose, old MHE model was induced via thioacetamide. label-free LC‒MS/MS method employed explore proteomic changes associated dysregulated biological pathways. A total 1533 proteins were identified, among these, 30 differentially expressed (18 upregulated, 12 downregulated). Three upregulated proteins, namely, fetuin-A, p23, intersectin-1 selected validated for their expression western blotting immunofluorescence analysis, which confirmed mass spectrometry results. These have not been reported previously cases. We also identified possible pathways Metascape, network analysis tool. found that may be involved generation precursor metabolites energy, neurotransmitter release cycle, positive regulation dendritic spine development, chaperone-mediated protein folding stabilization. This highlights potential mechanisms underlying neurological dysfunction identifies novel targets improved disease management.

Language: Английский

The 40S ribosomal subunit recycling complex modulates mitochondrial dynamics and endoplasmic reticulum - mitochondria tethering at mitochondrial fission/fusion hotspots DOI Creative Commons
Foozhan Tahmasebinia,

Yinglu Tang,

Rushi Tang

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 25, 2025

The 40S ribosomal subunit recycling pathway is an integral link in the cellular quality control network, occurring after translational errors have been corrected by ribosome-associated (RQC) machinery. Despite our understanding of its role, impact translation on metabolism remains poorly understood. Here, we reveal a conserved role (USP10-G3BP1) complex regulating mitochondrial dynamics and function. binds to fission-fusion proteins located at hotspots, functional assembly endoplasmic reticulum-mitochondria contact sites (ERMCSs). Furthermore, it alters activity mTORC1/2 pathways, suggesting between energy fluctuations. Effective communication essential for resolving proteostasis-related stresses. Our study illustrates that USP10-G3BP1 acts as hub interacts with various pathways adapt environmental stimuli promptly. It advances molecular RQC regulation helps explain pathogenesis human proteostasis dysfunction diseases. Coordinating protein supply significant challenge faced cells. authors found network may provide two.

Language: Английский

Citations

0
Investigating cognitive impairments and hippocampal proteome alterations in aged male rats with TAA-Induced minimal hepatic encephalopathy DOI
Vishal Vikram Singh, Shambhu Kumar Prasad, Arup Acharjee

et al.

Biogerontology, Journal Year: 2024, Volume and Issue: 26(1)

Published: Dec. 20, 2024

Language: Английский

Citations

1
Investigating Hippocampal Proteome Dynamics in Aging Rats with Minimal Hepatic Encephalopathy via High-Resolution Mass Spectrometry DOI Creative Commons
Vishal Vikram Singh, Shambhu Kumar Prasad, Arup Acharjee

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Abstract The aging population faces a gradual decline in physical and mental capacities, with an increased risk of liver cirrhosis chronic diseases leading to hepatic encephalopathy (HE). intertwining physiological manifestations the pathophysiology HE significantly impairs cognitive ability, reduces quality life, increases mortality. Hence, effective therapeutic intervention is imperative. present study investigated impact minimal (MHE) on impairment rat by analyzing hippocampal proteome dynamics. For this purpose, old MHE model was induced via thioacetamide. label-free LC‒MS/MS method employed explore proteomic changes associated dysregulated biological pathways. A total 1533 proteins were identified, among these, 30 differentially expressed (18 upregulated, 12 downregulated). Three upregulated proteins, namely, fetuin-A, p23, intersectin-1 selected validated for their expression western blotting immunofluorescence analysis, which confirmed mass spectrometry results. These have not been reported previously cases. We also identified possible pathways Metascape, network analysis tool. found that may be involved generation precursor metabolites energy, neurotransmitter release cycle, positive regulation dendritic spine development, chaperone-mediated protein folding stabilization. This highlights potential mechanisms underlying neurological dysfunction identifies novel targets improved disease management.

Language: Английский

Citations

0