Analysis of the Associations Between the Human Fecal Microbiome and Bone Density, Structure, and Strength: The Osteoporotic Fractures in Men (MrOS) Cohort DOI
Eric Orwoll,

Neeta Parimi,

Jack Wiedrick

et al.

Journal of Bone and Mineral Research, Journal Year: 2020, Volume and Issue: 37(4), P. 597 - 607

Published: Dec. 1, 2020

ABSTRACT In preclinical models, the composition and function of gut microbiota have been linked to bone growth homeostasis, but there are few available data from studies human populations. a hypothesis-generating experiment in large cohort community-dwelling older men (n = 831; age range, 78–98 years), we explored associations between fecal microbial profiles density, microarchitecture, strength measured with total hip dual-energy X-ray absorptiometry (DXA) high-resolution peripheral quantitative computed tomography (HRpQCT) (distal radius, distal diaphyseal tibia). Fecal samples were collected 16S rRNA gene V4 hypervariable region sequenced. Sequences bioinformatically processed through DADA2 pipeline then taxonomically assigned using SILVA. Generalized linear models as implemented microbiome multivariable association (MaAsLin 2) used test for skeletal measures specific genera. The abundances four bacterial genera weakly associated structure, or (false discovery rate [FDR] ≤ 0.05), directions generally consistent across multiple measures, supporting role on homeostasis. However, effect sizes small (log2 fold change < ±0.35), limiting power confidently identify these even high resolution imaging phenotypes, assessed resulting implications design future cohort-based studies. As analogous examples genomewide studies, find that larger will likely be needed health relying sequencing. Our findings bolster view is clinically important health, while also indicating challenges © 2022 American Society Bone Mineral Research (ASBMR).

Language: Английский

The intestinal flora of patients with GHPA affects the growth and the expression of PD-L1 of tumor DOI Creative Commons

Ding Nie,

Qiuyue Fang,

Jianhua Cheng

et al.

Cancer Immunology Immunotherapy, Journal Year: 2021, Volume and Issue: 71(5), P. 1233 - 1245

Published: Oct. 13, 2021

Pituitary adenoma (PA) is a common intracranial tumor. The evidence indicates that the tumor immune microenvironment (TIME) associated with PA and intestinal flora influences other tumors' growth through interacting TIME. However, how microbial contributes to development of response unknown.Here we used high-throughput Illumina MiSeq sequencing targeting V3-V4 region 16S ribosomal RNA gene investigate patients hormone-secreting pituitary (GHPA), nonfunctional (NFPA), healthy controls. We determined their effects on Fecal microbiota transplantation (FMT) was performed after adoptive transfer via peripheral blood mononuclear cells tumor-bearing nude mice, which allowed study response.We discovered differences in structures quantities between GHPA, NFPA, After FMT, GHPA promoted tumors mouse models. number programmed cell death ligand 1 (PD-L1)-positive increased tissues as well extent infiltration CD8+ cells. Increased numbers CD3+CD8+ levels sPD-L1 were detected blood.These findings indicated system may mediate this change.

Language: Английский

Citations

19
Composition of the microbiota in patients with growth hormone deficiency before and after treatment with growth hormone DOI Creative Commons

Patricia García Navas,

María Yolanda Ruiz del Prado,

Pablo Villoslada Blanco

et al.

Anales de Pediatría (English Edition), Journal Year: 2024, Volume and Issue: 100(6), P. 404 - 411

Published: May 27, 2024

Growth hormone (GH) and insulin-like growth factor-1 (IGF-1) have modulatory effects on bowel function its microbiota. Our aim was to investigate whether low levels of GH IGF-1 in patients with deficiency are associated changes gut physiology/integrity as well the composition

Language: Английский

Citations

2
Lactobacilli and Their Fermented Foods as a Promising Strategy for Enhancing Bone Mineral Density: A Review DOI
Weiwei He, Hanne Christine Bertram, Junyi Yin

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(32), P. 17730 - 17745

Published: July 30, 2024

Lactobacilli fermentation possesses special nutritional and health values to food, especially in improving diseases related the gut microbiota such as osteoporosis risk. Previous research indicates that lactobacilli-fermented foods have potential enhance bone mineral density (BMD), suggested by some clinical studies. Nonetheless, there is currently a lack of comprehensive summaries effects mechanisms on BMD. This review summarizes findings from preclinical studies, revealing lactobacilli possess mitigate age-related secondary factor-induced loss. Furthermore, these imply are likely mediated through modulation remodeling via inflammation-related pathways. Additionally, may augment calcium accessibility directly promoting absorption or modifying food constituents. Considering escalating global challenge bone-related issues among elderly population, this offer valuable reference for development strategies aimed at preventing osteoporosis.

Language: Английский

Citations

2
Alterations of the GH/IGF-I Axis and Gut Microbiome after Traumatic Brain Injury: A New Clinical Syndrome? DOI Open Access
Kevin C.J. Yuen, Brent E. Masel,

Kent Reifschneider

et al.

The Journal of Clinical Endocrinology & Metabolism, Journal Year: 2020, Volume and Issue: 105(9), P. e3054 - e3064

Published: June 25, 2020

Abstract Context Pituitary dysfunction with abnormal growth hormone (GH) secretion and neurocognitive deficits are common consequences of traumatic brain injury (TBI). Recognizing the comorbidity these symptoms is clinical importance; however, efficacious treatment currently lacking. Evidence Acquisition A review studies in PubMed published between January 1980 to March 2020 ongoing trials was conducted using search terms “growth hormone,” “traumatic injury,” “gut microbiome.” Synthesis Increasing evidence has implicated effects TBI promoting an interplay ischemia, cytotoxicity, inflammation that renders a subset patients develop postinjury hypopituitarism, severe fatigue, impaired cognition behavioral processes. Recent data have suggested association GH altered gut microbiome patients, thus prompting description hypothesized new syndrome called “brain associated fatigue cognition.” Notably, demonstrate distinct characteristics from those deficiency other non-TBI causes their symptom complex improves significantly recombinant human treatment, but does not reverse underlying mechanistic cause as typically recur upon cessation. Conclusion The reviewed describe importance alterations GH/insulin-like factor I axis after its influence bidirectional relationship, highlight may exist whom therapy could improve symptomatology. More needed further characterize this syndrome.

Language: Английский

Citations

18
Analysis of the Associations Between the Human Fecal Microbiome and Bone Density, Structure, and Strength: The Osteoporotic Fractures in Men (MrOS) Cohort DOI
Eric Orwoll,

Neeta Parimi,

Jack Wiedrick

et al.

Journal of Bone and Mineral Research, Journal Year: 2020, Volume and Issue: 37(4), P. 597 - 607

Published: Dec. 1, 2020

ABSTRACT In preclinical models, the composition and function of gut microbiota have been linked to bone growth homeostasis, but there are few available data from studies human populations. a hypothesis-generating experiment in large cohort community-dwelling older men (n = 831; age range, 78–98 years), we explored associations between fecal microbial profiles density, microarchitecture, strength measured with total hip dual-energy X-ray absorptiometry (DXA) high-resolution peripheral quantitative computed tomography (HRpQCT) (distal radius, distal diaphyseal tibia). Fecal samples were collected 16S rRNA gene V4 hypervariable region sequenced. Sequences bioinformatically processed through DADA2 pipeline then taxonomically assigned using SILVA. Generalized linear models as implemented microbiome multivariable association (MaAsLin 2) used test for skeletal measures specific genera. The abundances four bacterial genera weakly associated structure, or (false discovery rate [FDR] ≤ 0.05), directions generally consistent across multiple measures, supporting role on homeostasis. However, effect sizes small (log2 fold change < ±0.35), limiting power confidently identify these even high resolution imaging phenotypes, assessed resulting implications design future cohort-based studies. As analogous examples genomewide studies, find that larger will likely be needed health relying sequencing. Our findings bolster view is clinically important health, while also indicating challenges © 2022 American Society Bone Mineral Research (ASBMR).

Language: Английский

Citations

18