Xylocopa caerulea and Xylocopa auripennis harbor a homologous gut microbiome related to that of eusocial bees

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: May 17, 2023

Eusocial bees, such as bumblebees and honey harbor host-specific gut microbiota through their social behaviors. Conversely, the of solitary bees is erratic owing to lack eusocial activities. Carpenter (genus Xylocopa) are long-lived that do not exhibit advanced eusociality like bees. However, they often compete for nests reproduce. Xylocopa caerulea auripennis important pollinators wild plants on Hainan Island. Whether have bacteria in guts similar remains unknown.We targeted bacterial 16S rRNA V3-V4 region investigate diversity symbionts fore-midgut hindgut two carpenter X. auripennis.A maximum 4,429 unique amplicon sequence variants (ASVs) were detected from all samples, belonging 10 different phyla. shared community profiles, with Lactobacillaceae, Bifidobacteriaceae, Orbaceae being dominant entire guts. a highly conserved core set bacteria, including genera Candidatus Schmidhempelia Bombiscardovia. These taxa closely related those isolated bumblebees. The LEfSe analysis showed genus Bombilactobacillus significantly enriched hindguts both Functional prediction suggested most pathways involved carbohydrate lipid metabolism.Our results revealed structure confirmed presence some previously only found

Language: Английский

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Bacteria in honeybee crops are decoupled from those in floral nectar and bee mouths

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 5, 2024

Abstract Bacteria in the honeybee gut have garnered much attention as a factor affecting bee health. However, unlike hindgut, which has been primary focus of this research, crop, or honey stomach, is often assumed to be dominated by environmentally acquired transient taxa that matter little bees. To evaluate assumption, we examined bacterial crop and mouth foraging adults Apis mellifera A. cerana japonica floral nectar Japanese apricot, Prunus mume , visited bees Minabe-Tanabe region Japan. We found was distinct from both composition even though all samples were collected simultaneously at same locations, whereas indistinguishable each other. Furthermore, remained similar alpha beta diversity summer winter, showed sharp drop large increase winter. These results refute conventional suggesting instead contains conserved community largely environmental encounter they forage environment. also found, however, strains representative crop-associated species, Apilactobacillus kunkeei could season- host species-specific. Together, these findings suggest further studies communities are needed better understand relationship between honeybees their bacteria.

Language: Английский

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Microbiome diversity and composition across development stages of the Blue Orchard Bee, Osmia lignaria (Megachilidae)

Symbiosis, Journal Year: 2024, Volume and Issue: 93(3), P. 269 - 280

Published: July 1, 2024

Language: Английский

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Diet affects reproductive development and microbiota composition in honey bees

Animal Microbiome, Journal Year: 2024, Volume and Issue: 6(1)

Published: Nov. 5, 2024

Gut microbes are important to the health and fitness of many animals. Many factors have been shown affect gut microbial communities including diet, lifestyle, age. Most animals very complex physiologies, lifestyles, microbiomes, making it virtually impossible disentangle what largest impact on microbiota composition. Honeybees an excellent model study host-microbe interactions due their relatively simple microbiota, experimental tractability, eusociality. Worker honey bees distinct from queen mothers despite being close genetic relatives living in same environment. Queens workers differ numerous ways development, physiology, pheromone production, behavior. In prolonged absence a or Queen Mandibular Pheromones (QMP), some but not all will develop ovaries become "queen-like". Using this inducible developmental change, we aimed determine if diet and/or reproductive development impacts bee workers. Microbiota-depleted newly emerged were inoculated with mixture worker homogenates reared under four conditions varying exposure. Three weeks post-emergence, evaluated for ovary characterized. The proportion developed was increased QMP also when fed (royal jelly). Overall, found that rather than exposure, led more "queen-like" However, revealed alone cannot explain composition hypothesis explains differences between queens rejected. We evidence is one main drivers community compositions fully queens. Thus, predict behavioral other physiological dictate Our findings only contribute our understanding affecting which health, illustrate versatility benefits utilizing honeybees as system interactions.

Language: Английский

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Fecal transplant allows transmission of the gut microbiota in honey bees

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 29, 2023

Abstract The gut of honey bees is colonized by symbiotic bacteria during the first days adult life, once have emerged from their wax cells. Within five days, microbiota becomes remarkably stable and consistent across individual bees. Yet, modes acquisition transmission are to be confirmed. Few studies suggested could via contact with fecal matter in hive social interactions. However, composition still unknown. It particularly unclear whether all bacterial species can found viable feces therefore transmitted newborn nestmates. Using 16s rRNA gene amplicon sequencing we revealed that bee strikingly similar entire guts. We transplantation resulted microbial communities largely those obtained feeding homogenates. Our study shows sampling tools for longitudinal analysis community host-microbe results also imply young a plausible route core microbiota.

Language: Английский

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