Journal of Mathematical and Fundamental Sciences,
Journal Year:
2022,
Volume and Issue:
54(1), P. 190 - 210
Published: Oct. 18, 2022
Blister
beetles
are
an
important
object
of
study
in
the
field
agriculture
and
health.
Despite
being
known
to
be
for
host
animal,
gut
bacteria
blister
have
not
been
investigated
deeply.
This
aimed
provide
first
initial
description
bacterial
community
Mylabris
pustulata
as
one
species
beetles,
based
on
a
culture-independent
technique.
Adult
same
colony
were
sampled
confirmed
Thunberg.
The
content
was
used
high
throughput
sequencing,
targeting
V3-V4
regions
16S
rRNA
gene,
cultivation
resident
bacteria.
results
showed
that
higher
richness
present
communities
when
compared
grown
culture.
Proteobacteria
most
abundant
phylum
M.
pustulata,
whereby
reads
belonged
class
Gammaproteobacteria.
dominant
genera
determined
Enterobacter,
Acinetobacter,
Enterococcus,
Klebsiella,
Pseudomonas.
In
addition,
our
attempts
led
successful
isolation
members
Klebsiella
Enterococcus.
subject
this
could
explored
further
find
potential
roles
these
microbiota
specific
their
bioprospects.
mSystems,
Journal Year:
2024,
Volume and Issue:
9(7)
Published: July 23, 2024
ABSTRACT
Locusta
migratoria
is
an
important
phytophagous
pest,
and
its
gut
microbial
communities
play
role
in
cellulose
degradation.
In
this
study,
the
digestibility
dynamics
of
were
jointly
analyzed
using
high-throughput
sequencing
anthrone
colorimetry.
The
results
showed
that
diversity
across
life
stages
dynamically
changing.
species
richness
bacteria
was
significantly
higher
eggs
than
larvae
imago,
early
(first
second
instars)
late
(third
to
fifth
instars),
imago
larvae.
There
a
correlation
between
bacterial
digestibility.
Enterobacter
,
Lactococcus,
Pseudomonas
are
most
abundant
genera
throughout
all
stages.
Six
strains
highly
efficient
cellulolytic
screened,
which
dominant
bacteria.
Carboxymethyl
cellulase
activity
(CMCA)
filter
paper
(FPA)
experiments
revealed
had
highest
enzyme
activity.
This
study
provides
new
way
for
screening
lays
foundation
developing
insects
with
significant
biomass
into
cellulose-degrading
bioreactors.
IMPORTANCE
Cellulose
cheapest
renewable
resource
nature,
but
degradation
difficult,
so
finding
methods
urgent
challenge.
large
group
agricultural
pests,
number
microorganisms
inhabit
their
intestinal
tracts
We
combination
technology
changed
at
different
addition,
we
explored
community
uncovered
carboxymethyl
determined.
avenue
Insects,
Journal Year:
2023,
Volume and Issue:
14(6), P. 504 - 504
Published: May 31, 2023
Plutella
xylostella
(L.),
commonly
known
as
the
diamondback
moth,
is
currently
a
major
worldwide
pest.
Gut
bacteria
play
an
important
role
in
physiology
and
insecticide
resistance
of
P.
xylostella,
but
little
about
sources
transmission
routes
its
gut
bacteria.
In
this
study,
traditional
microbial
culture
methods
were
used
to
analyze
modes
which
could
help
develop
pest
control
strategies
based
on
The
main
findings
are
follows:
bacterial
diversity
was
significantly
higher
xylostella-fed
radish
sprouts
than
those
fed
artificial
diet,
indicating
potential
association
between
food
addition,
sequence
analysis
revealed
isolation
Enterobacter
sp.,
Pantoea
Cedecea
Pseudomonas
sp.
from
both
xylostella.
Importantly,
found
all
tested
samples
(radish
sprouts,
ovaries,
eggs),
suggesting
that
acquired
be
transferred
ovaries
eggs.
This
confirmed
through
experiments,
also
showed
eggs
carry
transmit
them
gut,
vertical
via
Furthermore,
3rd
instar
larvae
with
without
mixed
raised
until
4th
instar.
Then,
we
carried
same
bacteria,
can
horizontally
transmitted
social
behavior.
study
lays
foundation
for
further
exploration
sources,
transmission,
coevolution
host
provides
new
insights
into
source
Heliyon,
Journal Year:
2024,
Volume and Issue:
10(11), P. e32384 - e32384
Published: June 1, 2024
The
mutualistic
symbiotic
relationship
between
insects
and
bacteria
greatly
influences
the
growth
development
of
host
insects.
Tessaratoma
javanica
(Thunberg)
(Hemiptera:
Tessaratomidae),
also
referred
to
as
litchi
stink
bug,
has
recently
been
established
an
important
insect
pest
Litchi
chinensis
Sonn.
causes
substantial
yield
loss
in
India.
To
design
effective
environmentally
safe
management
strategies,
understanding
diversity
functions
microbiota
harbored
across
stages
is
very
important.
assessment
development-associated
T.
their
predicted
was
conducted
using
16S
rRNA
gene
sequences
obtained
by
Illumina
MiSeq
technology.
result
showed
that
taxonomic
analysis
associated
different
developmental
includes
a
total
46
phyla,
encompassing
139
classes,
271
orders,
474
families,
893
genera
bacteria.
All
shared
42.82
percent
operational
units
(OTUs),
with
97%
similarity
threshold.
Alpha
indices
maximum
species
richness
egg
adult
stages.
phyla
Proteobacteria
followed
Firmicutes,
Bacteriodetes,
Actinobacteria,
exhibited
highest
levels
abundance
all
javanica.
Microbiota
were
most
4th
nymphal
stage
(χ2
=711.67)
least
2nd
instars
=
44.45).
are
mainly
involved
amino
acid
metabolism,
cell
motility,
cellular
processes
signaling,
glycan
biosynthesis
lipid
membrane
transport.
present
study
documentation
information
on
life
will
prompt
novel
biological
strategies.
Biologia,
Journal Year:
2024,
Volume and Issue:
79(9), P. 2821 - 2830
Published: Aug. 2, 2024
Abstract
Gastrolina
depressa
(Coleoptera:
Chrysomelidae)
has
been
found
to
damage
many
walnut
plants
in
China.
It
prefers
feed
on
the
annual
leaves
of
an
important
economic
plant,
Pterocarya
stenoptera
(Fagales:
Juglandaceae),
and
causes
extensive
cosmetic
destruction,
even
plant
death.
Nevertheless,
its
biological
characteristics
are
mostly
unknown.
In
this
study,
we
investigate
harmful
G.
with
different
developmental
stages.
The
results
showed
that
had
four
stages:
egg,
larva
(1
st
-3
rd
instar),
pupa,
adult,
life
history
lasted
nine
days,
obvious
overlapping
generations.
food
intake
larvae
increased
increase
instar,
maximum
3
instar
was
448.19
mm
2
.
abdomen
females
enlarged
before
oviposition,
average
number
eggs
laid
47.63
±
13.53.
Bioassay
there
no
difference
search
time
for
young
(2.03,
1.25,
1.15,
0.91
min)
old
(1.68,
0.90,
1.93
P.
between
1
adults;
feeding
preference
adults
higher
than
(23.33%,
26.67%,
33.33%,
21.67%,
respectively).
successful
selection
rates
(81.67%,
80%,
85.00%,
70.00%,
respectively)
(8.35%,
11.67%,
3.34%,
PLoS ONE,
Journal Year:
2023,
Volume and Issue:
18(8), P. e0289733 - e0289733
Published: Aug. 17, 2023
Bacterial
content
of
mosquitoes
has
given
rise
to
the
development
innovative
tools
that
influence
and
seek
control
malaria
transmission.
This
study
identified
bacterial
microbiota
in
field-collected
female
adults
Anopheles
hyrcanus
group
three
species,
nivipes,
philippinensis,
vagus,
from
an
endemic
area
southeastern
part
Ubon
Ratchathani
Province,
northeastern
Thailand,
near
Lao
PDR-Cambodia-Thailand
border.
A
total
17
DNA
libraries
were
generated
pooled
abdomen
samples
(10
abdomens/
sample).
The
mosquito
was
characterized
through
analysis
sequences
V3-V4
regions
16S
rRNA
gene,
data
analyzed
QIIME2.
3,442
ASVs
obtained,
revealing
differences
both
within
same
species/group
between
different
species/group.
Statistical
difference
alpha
diversity
observed
An.
vagus
nivipes
beta
analyses
showed
community
most
dissimilar
other
species.
abundant
bacteria
belonged
Proteobacteria
phylum
(48%-75%)
which
Pseudomonas,
Serratia,
Pantoea
predominant
genera
among
four
However,
significantly
genus
each
as
follows:
Staphylococcus
group,
Rosenbergiella
Pseudomonas
vagus.
Particularly,
sp.
highly
all
species
except
nivipes.
present
provides
first
on
potential
vectors
a
starting
step
towards
understanding
role
biology
ultimately
for
control.
Journal of Apicultural Research,
Journal Year:
2023,
Volume and Issue:
unknown, P. 1 - 10
Published: Sept. 5, 2023
AbstractBumble
bees
and
honey
are
pollinivorous
nectarivorous
insects.
We
classify
them
as
“important
pollinators”
because
of
their
feeding
behaviors.
The
gut
microbiota
these
pollinators
is
essential
in
nutrition,
detoxification,
resistance
to
diseases.
Evaluating
the
diversity
microbiomes
for
pollinator
taxa
may
give
a
chance
understand
ecological
relationships
among
related
plants.
investigated
bacteria
fungi
communities
four
distributed
local
steppe-vegetation
habitat:
wild
populations
Bombus
terrestris,
niveatus
niveatus,
vorticosus
and,
manageable
pollinator,
Apis
mellifera.
composition
within
was
identified
using
Illumina
MiSeq
metabarcoding
ITS1
16S
rRNA
genes.
found
that
dominant
detected
phyla
three
bumble
bee
were
Firmicutes,
Bacteroidota,
Proteobacteria
while
A.
mellifera,
Proteobacteria,
respectively.
For
fungi,
Candida
predominant
taxa,
whereas
Penicillium,
Fusarium,
predominantly
mellifera.Keywords:
Wild
beehoney
beemicrobiomebacteriafungi
Code
availabilityNot
applicable.Disclosure
statementNo
potential
conflict
interest
reported
by
authors.Availability
data
material/data
availabilityRaw
sequencing
reads
have
been
deposited
at
NCBI
SRA
database
under
BioProject
PRJNA953022
PRJNA953027.Additional
informationFundingThis
work
supported
Hacettepe
University,
Project
No:
F.Bİ.P20.
Journal of Mathematical and Fundamental Sciences,
Journal Year:
2022,
Volume and Issue:
54(1), P. 190 - 210
Published: Oct. 18, 2022
Blister
beetles
are
an
important
object
of
study
in
the
field
agriculture
and
health.
Despite
being
known
to
be
for
host
animal,
gut
bacteria
blister
have
not
been
investigated
deeply.
This
aimed
provide
first
initial
description
bacterial
community
Mylabris
pustulata
as
one
species
beetles,
based
on
a
culture-independent
technique.
Adult
same
colony
were
sampled
confirmed
Thunberg.
The
content
was
used
high
throughput
sequencing,
targeting
V3-V4
regions
16S
rRNA
gene,
cultivation
resident
bacteria.
results
showed
that
higher
richness
present
communities
when
compared
grown
culture.
Proteobacteria
most
abundant
phylum
M.
pustulata,
whereby
reads
belonged
class
Gammaproteobacteria.
dominant
genera
determined
Enterobacter,
Acinetobacter,
Enterococcus,
Klebsiella,
Pseudomonas.
In
addition,
our
attempts
led
successful
isolation
members
Klebsiella
Enterococcus.
subject
this
could
explored
further
find
potential
roles
these
microbiota
specific
their
bioprospects.
