Lactic acid bacteria modulate the CncC pathway to enhance resistance to β-cypermethrin in the oriental fruit fly

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Abstract The gut microbiota of insects has been shown to regulate host detoxification enzymes. However, the potential regulatory mechanisms involved remain unknown. Here, we report that bacteria increase insecticide resistance by activating cap “n” collar isoform-C (CncC) pathway through enzymatically generated reactive oxygen species (ROS) in Bactrocera dorsalis. We demonstrated Enterococcus casseliflavus and Lactococcus lactis, two lactic acid-producing bacteria, B. dorsalis β-cypermethrin regulating cytochrome P450 (P450) enzymes α-glutathione S-transferase (GST) activities. These symbionts also induced expression CncC muscle aponeurosis fibromatosis. BdCncC knockdown led a decrease caused bacteria. Ingestion ROS scavenger vitamin C resistant strain affected BdCncC/BdKeap1/BdMafK, resulting reduced GST activity. Furthermore, feeding with E. or L. lactis showed BdNOX5 increased production, BdCncC/BdMafK genes. Moreover, acid activated ROS-associated regulation Collectively, our findings indicate symbiotic modulate intestinal pathways affecting physiological biochemistry, thus providing new insights into involvement insect microbes development resistance.

Language: Английский

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Uninheritable but Widespread Bacterial Symbiont Enterococcus casseliflavus Mediates Detoxification of the Insecticide Chlorantraniliprole in the Agricultural Invasive Pest Spodoptera frugiperda

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(33), P. 18365 - 18377

Published: Aug. 6, 2024

Host-symbiont interaction plays a crucial role in determining the host's fitness under toxic stress, as observed numerous insect species. However, mechanism of symbionts involved detoxification insecticides remains poorly known. In this study, through microbiome, proteomic, and genomic analysis, we identified prevalent symbiont,

Language: Английский

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Zirconium based Metal-Organic framework for emamectin benzoate Delivery: Enhanced control efficiency against Spodoptera frugiperda (Lepidoptera: Noctuidae) and significantly improved biosafety

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 159547 - 159547

Published: Jan. 1, 2025

Language: Английский

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Gut bacteria of Spodoptera frugiperda establish endophytic association and affect the interactions of their host herbivore with maize plants

Journal of Pest Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Language: Английский

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The larval gut of Spodoptera frugiperda harbours culturable bacteria with metabolic versatility after insecticide exposure

Insect Molecular Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 14, 2025

Abstract Spodoptera frugiperda (fall armyworm) poses a substantial risk to crops worldwide, resulting in considerable economic damage. The gut microbiota of insects plays crucial roles digestion, nutrition, immunity, growth and, sometimes, the degradation insecticides. current study examines effect synthetic insecticides on microbiome third instar S. larvae using both culture‐dependent techniques and 16S rRNA gene sequencing for bacterial community profiling diversity analysis. In untreated larvae, approach revealed diverse dominated by phyla Firmicutes, Proteobacteria Bacteroidota, with key genera including Bacteroides , Faecalibacterium Pelomonas . parallel, 323 strains were isolated assigned orders Bacillales, Burkholderiales, Enterobacterales, Flavobacteriales, Lactobacillales, Micrococcales, Neisseriaies, Pseudomonadales, Sphingobacteriales Xanthomonadales. prevailing culturable species included Serratia marcescens Klebsiella variicola Enterobacter quasiroggenkampii Treatment sublethal concentrations three (broflanilide, spinosad indoxacarb) caused significant changes composition. Treated showed shift towards increased abundance decreased Firmicutes. Specifically, Acinetobacter Rhodococcus dominant treated samples. Functional predictions highlighted metabolic versatility involving nutrient processing, immune response, detoxification, xenobiotic metabolism, stress suggesting microbial adaptation insecticide exposure. Network correlation analysis disrupted interactions altered structures under treatment. These findings enhance our understanding how impact may inform future strategies managing pest resistance through microbiome‐based approaches.

Language: Английский

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Host diet and phylogeny interact to shape the bacterial and fungal microbiome in the regurgitant of four Spodoptera species

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

The gut microbiome of Lepidopteran insects is highly dynamic, influenced by both host diet and phylogeny. While microbial communities are thought to facilitate adaptation diverse diets environments, the existence a core shared among closely related herbivores remains largely untested. In this study, we examined in regurgitant four Spodoptera species (S. exigua, S. frugiperda, latifascia, littoralis) across different (artificial diet, cotton, maize, squash). Using high-throughput sequencing, characterized bacterial fungal community composition diversity. Bacterial were shaped species, indicating species-specific selection. contrast, exclusively structured with lower diversity dominance few key taxa. Notably, no operational taxonomic units consistently all or diets, challenging concept conserved these generalist herbivores. Understanding how shape herbivores’ ability feed on plants may offer potential strategies for microbiome-based pest management.

Language: Английский

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Distinct effects of two dominant enteric bacteria on the developmental performance of spodoptera frugiperda and their association with physiological metabolism

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 27, 2025

Enteric bacteria can play an important role in the developmental performance of their insect hosts. The present study revealed that two dominant enteric bacteria, Enterococcus mundtii and casseliflavus, are gut Spodoptera frugiperda larvae on different host plants (maize rice). However, S. remains poorly understood. To clarify functions E. effects growth development were studied by separately adding them to artificial diet with proportions yeast. elucidate physiological metabolism underlying differential these frugiperda, transcriptome sequencing was conducted. results showed under a rich (with 1.85% yeast extract), casseliflavus significantly inhibited larval prolonged pupal stage, poor (without survival rates decreased, but body weight increased, increased. had no significant effect fed nutritionally or diet. These indicate exerts nutrient-dependent life history traits, while munditi has little impact frugiperda. Transcriptome analysis gene expression suppression genes related carbohydrate transport casseliflavus. For instance, downregulation UDP-glycosyltransferase (UGT) amino acid is closely associated frugiperda.These findings provide deeper insights into its

Language: Английский

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Metagenomic profiling of gut microbiota in Fall Armyworm (Spodoptera frugiperda) larvae fed on different host plants

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Sept. 11, 2024

Language: Английский

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Transgenerational effect of a tea saponin–matrine mixture promotes fecundity and alters associated bacteria in the fall armyworm Spodoptera frugiperda

Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 213, P. 118472 - 118472

Published: March 29, 2024

Language: Английский

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Role of Gut Bacteria in Enhancing Host Adaptation of Tuta absoluta to Different Host Plants

Insects, Journal Year: 2024, Volume and Issue: 15(10), P. 795 - 795

Published: Oct. 13, 2024

The insect gut bacteria play important roles in development and growth, such as immune defense, nutrient metabolism, regulating adaptations for plants, etc.

Language: Английский

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