Effects of life history strategy on the diversity and composition of the coral holobiont communities of Sabah, Malaysia

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 6, 2025

Coral-associated microbes have essential roles in promoting and regulating host function health. As climate change advances other environmental perturbations increasingly impact corals, it is becoming ever more important that we understand the composition of microbial communities hosted. Without this baseline impossible to assess magnitude direction any future changes community structure. Here, characterised both bacterial Symbiodiniaceae four coral species (Diploastrea heliopora, Porites lutea, Pachyseris speciosa, Pocillopora acuta) collected from Sabah, Malaysia. Our findings reveal distinct associated with different tending reflect varied life history strategies their hosts. Microbial could be differentiated by collection site, shifts towards stress tolerant types seen samples on shallow Sunda Shelf. Additionally, identified a core microbiome within discrete between all species. We show are structured appear influenced characteristics. Furthermore, for each finding several amplicon sequence variants were shared hosts, suggests key role health regardless identity. Given paucity work performed megadiverse regions such as Coral Triangle, research takes increased importance our efforts how holobiont functions altered advances.

Language: Английский

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Herbicide prometryn aggravates the detrimental effects of heat stress on the potential for mutualism of Symbiodiniaceae

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: unknown, P. 137389 - 137389

Published: Jan. 1, 2025

Language: Английский

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Combination of Sample Preservation Approaches and DNA Extraction Methods for Long‐Read Sequencing of Nudibranchs' Genomes

Ecology and Evolution, Journal Year: 2025, Volume and Issue: 15(4)

Published: April 1, 2025

ABSTRACT With the increasing interest in whole genome sequencing of eukaryotes, it is becoming evident that selecting most suitable high molecular weight DNA extraction method crucial for maximizing benefits long‐read technologies. However, many species cannot be processed immediately at sampling site due to remoteness location, necessitating tissue preservation may affect fragment size. This study aimed identify combination four approaches and six methods ensure DNA. A single Peltodoris atromaculata (Nudibranchia) specimen was sliced into ∼30 mg sub‐samples, ensuring consistency across 24 preservation‐extraction combinations triplicates. Samples were either stored 4°C, dried room temperature, flash‐frozen liquid nitrogen, or preserved ethanol −20°C. Afterward, they using five commercially available kits specific extraction, as well a custom protocol. Three aspects quality evaluated: total yield, size distribution, availability amplification. Most yielded optimal results only some three aspects. We identified sequencing: CTAB‐based protocol applied frozen samples, Wizard (Promega) Nanobind (PacBio) both ethanol‐preserved paired with Monarch (NEB) kits. The suitability selected confirmed by PacBio sequencing, producing yield 3.6 Gbp (3.2x estimated coverage). indicate success extractions influenced methods. Although tested on nudibranchs, these findings are highly useful genomic studies other organisms, which need remote locations before being transported laboratory processing.

Language: Английский

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Coral‐associated Symbiodiniaceae exhibit host specificity but lack phylosymbiosis, with Cladocopium and Durusdinium showing different cophylogenetic patterns

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: May 3, 2025

Summary Altering the composition of Symbiodiniaceae community to adapt anomalous sea water warming represents a potential survival mechanism for scleractinian corals. However, processes assembly and long‐standing evolution coral–Symbiodiniaceae interactions remain unclear. Here, we utilized ITS2 (internal transcribed spacer 2) amplicon sequencing SymPortal framework investigate diversity specificity across 39 coral species. Furthermore, tested phylosymbiosis cophylogeny between hosts their Symbiodiniaceae. In our study, environmental samples exhibited highest diversity. Cladocopium Durusdinium dominated communities, with significant β‐diversity differences among Additionally, host was widespread in Symbiodiniaceae, especially spp., yet lacked phylosymbiotic pattern. Moreover, spp. showed cophylogenetic congruence hosts, while there no evidence switching predominant evolutionary event, implying its contribution diversification. These findings suggest that does not recapitulate phylogeny, alone drive or cophylogeny. As reservoirs, free‐living may influence symbiotic communities. Durusdinium– associations lack signals, indicating more flexible partnerships than . Overall, results enhance understanding interactions.

Language: Английский

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