Implication of the HLA-DQA1, HLA-DQB1 and CTLA-4 alleles in the susceptibility to type 1 diabetes in Jordanian population DOI Creative Commons

Monther Hussain Radi Obaied,

Nazmi Özer,

Hussein Alawneh

et al.

Molecular Biology Reports, Journal Year: 2025, Volume and Issue: 52(1)

Published: March 21, 2025

Abstract Background Type 1 diabetes mellitus (T1D) is a chronic autoimmune disease caused by the selective destruction of pancreatic beta cells, leading to insulin deficiency. Both genetic and environmental factors contribute susceptibility. Among factors, human leukocyte antigen (HLA) class II molecules, particulary DQA1 DQB1 haplotypes, have been associated with T1D risk. This study aimed identify haplotypes that increase susceptibility or provide protection against in Jordanian population. Methods A total 200 healthy individuals patients were included study. Genomic DNA was extracted from blood samples HLA-DQA1, HLA-DQB1 cytotoxic T-lymphocyte protein 4 (CTLA-4) gene regions amplified PCR. The PCR products then subjected restriction enzyme digestion analyzed through agarose gel electrophoresis determine different haplotypes. Results HLA-DQA1*01:01 found be significantly increased T1D. In contrast, HLA-DQA1*02:01 HLA-DQB1*05:01 appeared protective effects No significant differences observed for other between control patient groups. Additionally, no difference has terms CTLA-4 polymorphisms. Conclusion These findings suggest may serve as marker susceptibility, while confer protectionin Identifying these risk could early prevention strategies advanced research into additional markers

Language: Английский

Implication of the HLA-DQA1, HLA-DQB1 and CTLA-4 alleles in the susceptibility to type 1 diabetes in Jordanian population DOI Creative Commons

Monther Hussain Radi Obaied,

Nazmi Özer,

Hussein Alawneh

et al.

Molecular Biology Reports, Journal Year: 2025, Volume and Issue: 52(1)

Published: March 21, 2025

Abstract Background Type 1 diabetes mellitus (T1D) is a chronic autoimmune disease caused by the selective destruction of pancreatic beta cells, leading to insulin deficiency. Both genetic and environmental factors contribute susceptibility. Among factors, human leukocyte antigen (HLA) class II molecules, particulary DQA1 DQB1 haplotypes, have been associated with T1D risk. This study aimed identify haplotypes that increase susceptibility or provide protection against in Jordanian population. Methods A total 200 healthy individuals patients were included study. Genomic DNA was extracted from blood samples HLA-DQA1, HLA-DQB1 cytotoxic T-lymphocyte protein 4 (CTLA-4) gene regions amplified PCR. The PCR products then subjected restriction enzyme digestion analyzed through agarose gel electrophoresis determine different haplotypes. Results HLA-DQA1*01:01 found be significantly increased T1D. In contrast, HLA-DQA1*02:01 HLA-DQB1*05:01 appeared protective effects No significant differences observed for other between control patient groups. Additionally, no difference has terms CTLA-4 polymorphisms. Conclusion These findings suggest may serve as marker susceptibility, while confer protectionin Identifying these risk could early prevention strategies advanced research into additional markers

Language: Английский

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