Characterization of BbKlf1 as a novel transcription factor vital for asexual and infection cycles of Beauveria bassiana DOI
Rehab Abdelmonem Mohamed,

Chong‐Tao Guo,

Siyuan Xu

et al.

Environmental Microbiology Reports, Journal Year: 2022, Volume and Issue: 14(5), P. 719 - 731

Published: July 19, 2022

The large family of C2H2-type zinc finger transcription factors (TFs) comprise the Kruppel-like (KLFs) that evolved relatively late in eukaryotes but remain unexplored filamentous fungi. Here, we report an orthologue (BbKlf1) yeast Klf1 mediating cell wall integrity (CWI) is a wide-spectrum TF evidently localized nucleus and cytoplasm Beauveria bassiana. BbKlf1 features conserved domains multiple DNA-binding motifs predicted to bind promoter DNA fragments target genes across asexual developmental stress-responsive pathways. Despite limited impact on normal colony growth, deletion Bbklf1 resulted impaired CWI hypersensitivity Congo red-induced stress. Also, mutant was severely compromised tolerance oxidative osmotic stresses, hyphal septation differentiation, conidiation capacity (reduced by 95%), conidial quality (viability hydrocarbon epitope pattern) virulence. Importantly, these phenotypes correlated well with sharply repressed or nearly abolished expressions those required for involved chitin biosynthesis, antioxidant activity, division aerial maturation. These findings indicate essentiality insect-pathogenic lifecycles B. bassiana novel scenario much beyond orthologue-mediated CWI, suggesting important roles its orthologues

Language: Английский

A life-and-death struggle: interaction of insects with entomopathogenic fungi across various infection stages DOI Creative Commons

Meiqi Ma,

Jing Luo, Chong Li

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 8, 2024

Insects constitute approximately 75% of the world’s recognized fauna, with majority species considered as pests. Entomopathogenic fungi (EPF) are parasitic microorganisms capable efficiently infecting insects, rendering them potent biopesticides. In response to infections, insects have evolved diverse defense mechanisms, prompting EPF develop a variety strategies overcome or circumvent host defenses. While interaction mechanisms between and is well established, recent findings underscore that their interplay more intricate than previously thought, especially evident across different stages infection. This review primarily focuses on insect strategies, centered around three infection stages: (1) Early stage: involving pre-contact detection avoidance behavior in along induction behavioral responses upon contact cuticle; (2) Penetration intra-hemolymph growth initiation cellular humoral immune functions while symbiotic microbes can further contribute resistance; (3) Host insect’s death ultimate confrontation pathogens insects. Infected strive separate themselves from healthy population, rely infected spread new hosts. Also, we discuss novel pest management strategy underlying cooperation disturbing system. By enhancing our understanding insect, this provides perspectives for EPF-mediated developing effective fungal insecticides.

Language: Английский

Citations

44
Fungal infection of insects: molecular insights and prospects DOI Open Access
Hong Song, Junmei Shang,

Yaneli Sun

et al.

Trends in Microbiology, Journal Year: 2023, Volume and Issue: 32(3), P. 302 - 316

Published: Sept. 29, 2023

Language: Английский

Citations

37
A novel partitivirus orchestrates conidiation, stress response, pathogenicity, and secondary metabolism of the entomopathogenic fungus Metarhizium majus DOI Creative Commons
Ping Wang, Guogen Yang,

Najie Shi

et al.

PLoS Pathogens, Journal Year: 2023, Volume and Issue: 19(5), P. e1011397 - e1011397

Published: May 22, 2023

Mycoviruses are widely present in all major groups of fungi but those entomopathogenic Metarhizium spp. remain understudied. In this investigation, a novel double-stranded (ds) RNA virus is isolated from majus and named partitivirus 1 (MmPV1). The complete genome sequence MmPV1 comprises two monocistronic dsRNA segments (dsRNA 2), which encode an RNA-dependent polymerase (RdRp) capsid protein (CP), respectively. classified as new member the genus Gammapartitivirus family Partitiviridae based on phylogenetic analysis. As compared to MmPV1-free strain, isogenic MmPV1-infected single-spore isolates were compromised terms conidiation, tolerance heat shock UV-B irradiation, while these phenotypes accompanied by transcriptional suppression multiple genes involved response DNA damage repair. attenuated fungal virulence since infection resulted reduced hydrophobicity, adhesion, cuticular penetration. Additionally, secondary metabolites significantly altered infection, including production triterpenoids, metarhizins A B, increased nitrogen phosphorus compounds. However, expression individual proteins M. had no impact host phenotype, suggesting insubstantive links between defective single viral protein. These findings indicate that decreases fitness its environment insect-pathogenic lifestyle through orchestration stress tolerance, pathogenicity, metabolism.

Language: Английский

Citations

24
Fights on the surface prior to fungal invasion of insects DOI Creative Commons
Junmei Shang, Hong Song, Chengshu Wang

et al.

PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(2), P. e1011994 - e1011994

Published: Feb. 22, 2024

Entomopathogenic fungi (EPF) infect insects by landing on and penetrating cuticles. Emerging evidence has shown that, prior to the invasion of insects, fungal cells have battle overcome diverse challenges, including host behavioral defenses, colonization resistance mediated ectomicrobiotas, recognition, generation enough penetration pressure. The ascomycete EPF such as Metarhizium Beauveria can thus produce adhesive proteins and/or exopolysaccharide mucilage tightly glue Producing antimicrobial peptides chemical compounds enable outcompete cuticular defensive microbes. use divergent membrane receptors, accumulation, quick degradation lipid droplets in conidial help recognize proper hosts build up cellular turgor breach cuticles for systematic invasion. Further investigations are still required unveil multifaceted intricate relationships between insect hosts.

Language: Английский

Citations

8
Genomic Determinants of Entomopathogenic Fungi and Their Involvement in Pathogenesis DOI

Ravindra P. Vidhate,

Vishal V. Dawkar, Sachin A. Punekar

et al.

Microbial Ecology, Journal Year: 2022, Volume and Issue: 85(1), P. 49 - 60

Published: Jan. 3, 2022

Language: Английский

Citations

24
Coffee-Associated Endophytes: Plant Growth Promotion and Crop Protection DOI Creative Commons
Suhail Asad, Alviti Kankanamalage Hasith Priyashantha, Saowaluck Tibpromma

et al.

Biology, Journal Year: 2023, Volume and Issue: 12(7), P. 911 - 911

Published: June 25, 2023

Endophytic microbes are a ubiquitous group of plant-associated communities that colonize the intercellular or intracellular host tissues while providing numerous beneficial effects to plants. All plant species thought be associated with endophytes, majorly constituted bacteria and fungi. During last two decades, there has been considerable movement toward study endophytes coffee In this review, main consideration is given address coffee-associated endophytic fungi, particularly their action on growth promotion biocontrol pests. addition, we sought identify analyze gaps in available research. Additionally, potential improve quality seeds briefly discussed. Even though limited studies subject, potentiality through enhancing nitrogen fixation, availability minerals, nutrient absorption, secretion phytohormones, other bioactive metabolites well recognized. Further, antagonistic effect against various pathogenic bacteria, nematodes, also insect pests leads protection crop. Furthermore, it recognized enhance sensory characteristics as new field study.

Language: Английский

Citations

10
Fus3/Kss1‐MAP kinase and Ste12‐like control distinct biocontrol‐traits besides regulation of insect cuticle penetration via phosphorylation cascade in a filamentous fungal pathogen DOI
Xin Zhao,

Yahui Jiang,

Huifang Wang

et al.

Pest Management Science, Journal Year: 2023, Volume and Issue: 79(7), P. 2611 - 2624

Published: March 9, 2023

Homolog of the yeast Fus3/Kss1 mitogen-activated protein kinase (MAPK) pathway and its target transcription factor, Ste12-like, are involved in penetration host cuticle/pathogenicity many ascomycete pathogens. However, details their interaction during fungal infection, as well controlled other virulence-associated traits, unclear.Ste12-like (BbSte12) MAPK homolog (Bbmpk1) interacted nucleus, phosphorylation BbSte12 by Bbmpk1 was essential for insect cuticle an pathogen, Beauveria bassiana. some distinct biocontrol-traits were found to be mediated Ste12 Bbmpk1. In contrast ΔBbmpk1 colony that grew more rapid than wild-type strain, inactivation resulted opposite phenotype, which consistent with different proliferation rates hemocoel after direct injection conidia bypass cuticle. Reduced conidial yield decreased hydrophobicity examined both mutants, however they displayed conidiogenesis, accompanying differently altered cell cycle, hyphal branching septum formation. Moreover, showed increased tolerance oxidative agent, whereas phenotype seen ΔBbSte12 strain. RNA sequencing analysis revealed 356 genes depending on penetration, but 1077 584 independently BbSte12.BbSte12 separately participate additional pathways control conidiation, growth differentiation, stress response besides regulating via cascade. © 2023 Society Chemical Industry.

Language: Английский

Citations

9
Rad6, a ubiquitin conjugator required for insect-pathogenic lifestyle, UV damage repair, and genomic expression of Beauveria bassiana DOI

Xin-Cheng Luo,

Lei Yu, Siyuan Xu

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 281, P. 127622 - 127622

Published: Jan. 18, 2024

Language: Английский

Citations

3
Differential Roles of Five Fluffy Genes (flbA–flbE) in the Lifecycle In Vitro and In Vivo of the Insect–Pathogenic Fungus Beauveria bassiana DOI Creative Commons

Chong‐Tao Guo,

Xin-Cheng Luo,

Sheng‐Hua Ying

et al.

Journal of Fungi, Journal Year: 2022, Volume and Issue: 8(4), P. 334 - 334

Published: March 23, 2022

The fluffy genes flbA-flbE are well-known players in the upstream developmental activation pathway that activates key gene brlA of central (CDP) to initiate conidiation Aspergillus nidulans. Here, we report insignificant roles their orthologs radial growth Beauveria bassiana under normal culture conditions and different stresses although flbA flbD were involved respective responses heat shock H2O2. Aerial level was lowered deletion mutants flbB flbE (~15%) less than flbC (~30%), which CDP abaA repressed consistently during incubation. CDP-controlled blastospore production submerged cultures mimicking insect hemolymph abolished mutant with being sharply repressed, decreased by 55% only downregulated. fungal virulence against a model attenuated absence more irrespective cuticle infection or cuticle-bypassing (intrahemocoel injection). These findings unravel important role flbC, but null flbB/D/E, B. bassiana's insect-pathogenic lifecycle scenario distinctive from A.nidulans.

Language: Английский

Citations

14
UV‐induced mutagenesis of Beauveria bassiana (Hypocreales: Clavicipitaceae) yields two hypervirulent isolates with different transcriptomic profiles DOI

Yanxia Sun,

Bang‐Xian Zhang,

Wenting Zhang

et al.

Pest Management Science, Journal Year: 2023, Volume and Issue: 79(8), P. 2762 - 2779

Published: March 14, 2023

The fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) can infest over 300 plant species and cause huge economic losses. Beauveria bassiana (Hypocreales: Clavicipitaceae) is one of the most widely used entomopathogenic fungi (EPF). Unfortunately, efficacy B. against S. quite low. Hypervirulent EPF isolates be obtained by ultraviolet (UV)-irradiation. Here we report on UV-induced mutagenesis transcriptomic analysis bassiana.The wild-type (WT) (ARSEF2860) was exposed to UV light induce mutagenesis. Two mutants (named 6M 8M) showed higher growth rates, conidial yields, germination rates compared WT strain. levels tolerance osmotic, oxidative, stresses. protease, chitinase, cellulose, chitinase activities than WT. Both were compatible with insecticides matrine, spinetoram, chlorantraniliprole, but incompatible emamectin benzoate. Insect bioassays that both more virulent greater wax moth Galleria mellonella. Transcriptomic profiles determined RNA-sequencing. differentially expressed genes (DEGs) identified. gene set enrichment (GSEA), protein-protein interaction (PPI) network, hub revealed virulence-related genes.Our data demonstrate UV-irradiation a very efficient economical technique improve virulence stress resistance bassiana. Comparative provide insights into genes. These results new ideas for improving genetic engineering field EPF. © 2023 Society Chemical Industry.

Language: Английский

Citations

8