Characterization of BbKlf1 as a novel transcription factor vital for asexual and infection cycles of Beauveria bassiana DOI
Rehab Abdelmonem Mohamed,

Chong‐Tao Guo,

Siyuan Xu

et al.

Environmental Microbiology Reports, Journal Year: 2022, Volume and Issue: 14(5), P. 719 - 731

Published: July 19, 2022

The large family of C2H2-type zinc finger transcription factors (TFs) comprise the Kruppel-like (KLFs) that evolved relatively late in eukaryotes but remain unexplored filamentous fungi. Here, we report an orthologue (BbKlf1) yeast Klf1 mediating cell wall integrity (CWI) is a wide-spectrum TF evidently localized nucleus and cytoplasm Beauveria bassiana. BbKlf1 features conserved domains multiple DNA-binding motifs predicted to bind promoter DNA fragments target genes across asexual developmental stress-responsive pathways. Despite limited impact on normal colony growth, deletion Bbklf1 resulted impaired CWI hypersensitivity Congo red-induced stress. Also, mutant was severely compromised tolerance oxidative osmotic stresses, hyphal septation differentiation, conidiation capacity (reduced by 95%), conidial quality (viability hydrocarbon epitope pattern) virulence. Importantly, these phenotypes correlated well with sharply repressed or nearly abolished expressions those required for involved chitin biosynthesis, antioxidant activity, division aerial maturation. These findings indicate essentiality insect-pathogenic lifecycles B. bassiana novel scenario much beyond orthologue-mediated CWI, suggesting important roles its orthologues

Language: Английский

The carbon catabolite repressor CreA is an essential virulence factor of Metarhizium acridum against Locusta migratoria DOI
Dongxu Song,

Yumei Jin,

Youhui Shi

et al.

Pest Management Science, Journal Year: 2022, Volume and Issue: 78(8), P. 3676 - 3684

Published: May 25, 2022

Abstract BACKGROUND CreA has been proved to be a core gene in asexual conidiation Metarhizium acridum , which regulates the shift of normal and microcycle conidiation. At present, research on fungi focused carbon source metabolism. There is lack effect virulence pathogenic fungi. RESULTS The MaCreA disrupted strain (Δ ) for Locusta migratoria was lost by topical inoculation bioassay. formation rate turgor pressure appressoria decreased. Growth Δ host hemolymph delayed, number hyphal bodies significantly reduced. conidial cell wall became thicker, mannan content decreased, chitin increased significantly, it more sensitive calcofluor white Congo Red. α‐1,3‐Glucan β‐1,3‐glucan are exposed surface conidia than wild type. Lmspätzle Lmcactus immune response genes Toll pathway, showed stronger transcriptional activities at early stage invasion. phenoloxidase activity assay also immunostimulation vitro . CONCLUSION main reasons loss were reduced penetration ability appressoria, limited growth insect © 2022 Society Chemical Industry.

Language: Английский

Citations

4
Three Small Cysteine-Free Proteins (CFP1–3) Are Required for Insect-Pathogenic Lifestyle of Metarhizium robertsii DOI Creative Commons

Ya‐Ni Mou,

Kang Ren,

Siyuan Xu

et al.

Journal of Fungi, Journal Year: 2022, Volume and Issue: 8(6), P. 606 - 606

Published: June 6, 2022

Unique CFP (cysteine-free protein; 120 aa) has been identified as an extraordinary virulence factor in Beauveria bassiana (Cordycipitaceae), a main source of wide-spectrum fungal insecticides. Its homologs exclusively exist insect pathogens Hypocreales, suggesting their importance for insect-pathogenic lifestyle. In this study, all three (CFP1-3, 128-145 were proven essential factors Metarhizium robertsii (Clavicipitaceae). Despite limited effects on asexual cycles vitro, knockout mutants cfp1,cfp2 and cfp3 severely compromised capability normal cuticle infection, which most tested Galleria mellonella larvae survived. The blocked infection concurred with reduced secretion extracellular enzymes, including Pr1 proteases required penetration. Cuticle-bypassing by intrahemocoel injection ~250 conidia per larva resulted greater reduction the mutant cfp1 (82%) than cfp2 (21%) or (25%) versus parental wild-type. Transcriptomic analysis revealed dysregulation 604 genes (up/down ratio: 251:353) Δcfp1 mutant. Many them involved virulence-related cellular processes events aside from 154 functionally unknown 56:98). These results reinforce roles small hypocrealean adaptation to lifestyle exploitability genetic improvement insecticidal activity.

Language: Английский

Citations

4
Conserved and Noncanonical Activities of Two Histone H3K36 Methyltransferases Required for Insect-Pathogenic Lifestyle of Beauveria bassiana DOI Creative Commons

Kang Ren,

Ya‐Ni Mou,

Sheng‐Hua Ying

et al.

Journal of Fungi, Journal Year: 2021, Volume and Issue: 7(11), P. 956 - 956

Published: Nov. 11, 2021

Set2 and Ash1 are histone methyltransferases (KMTs) in the KMT3 family normally used to catalyze methylation of H3K36 (H3K36me) but remain unexplored fungal insect pathogens. Here, we report broader/greater roles mono-/di-/trimethylation (me1/me2/me3) H3K4 than

Language: Английский

Citations

5
Topical Infection of Cordyceps militaris in Silkworm Larvae Through the Cuticle has Lower Infectivity Compared to Beauveria bassiana and Metarhizium anisopliae DOI
Tatsuya Kato,

S. Inagaki,

Chikashi Shibata

et al.

Current Microbiology, Journal Year: 2024, Volume and Issue: 82(1)

Published: Dec. 2, 2024

Language: Английский

Citations

0
Characterization of BbKlf1 as a novel transcription factor vital for asexual and infection cycles of Beauveria bassiana DOI
Rehab Abdelmonem Mohamed,

Chong‐Tao Guo,

Siyuan Xu

et al.

Environmental Microbiology Reports, Journal Year: 2022, Volume and Issue: 14(5), P. 719 - 731

Published: July 19, 2022

The large family of C2H2-type zinc finger transcription factors (TFs) comprise the Kruppel-like (KLFs) that evolved relatively late in eukaryotes but remain unexplored filamentous fungi. Here, we report an orthologue (BbKlf1) yeast Klf1 mediating cell wall integrity (CWI) is a wide-spectrum TF evidently localized nucleus and cytoplasm Beauveria bassiana. BbKlf1 features conserved domains multiple DNA-binding motifs predicted to bind promoter DNA fragments target genes across asexual developmental stress-responsive pathways. Despite limited impact on normal colony growth, deletion Bbklf1 resulted impaired CWI hypersensitivity Congo red-induced stress. Also, mutant was severely compromised tolerance oxidative osmotic stresses, hyphal septation differentiation, conidiation capacity (reduced by 95%), conidial quality (viability hydrocarbon epitope pattern) virulence. Importantly, these phenotypes correlated well with sharply repressed or nearly abolished expressions those required for involved chitin biosynthesis, antioxidant activity, division aerial maturation. These findings indicate essentiality insect-pathogenic lifecycles B. bassiana novel scenario much beyond orthologue-mediated CWI, suggesting important roles its orthologues

Language: Английский

Citations

1