Exploring Microorganisms from Plastic-Polluted Sites: Unveiling Plastic Degradation and PHA Production Potential DOI Creative Commons
Diana A. Garza Herrera, Marija Mojićević, Brana Pantelic

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(12), P. 2914 - 2914

Published: Dec. 3, 2023

The exposure of microorganisms to conventional plastics is a relatively recent occurrence, affording limited time for evolutionary adaptation. As part the EU-funded project BioICEP, this study delves into plastic degradation potential isolated from sites with prolonged pollution, such as plastic-polluted forests, biopolymer-contaminated soil, oil-contaminated municipal landfill, but also distinctive soil sample pieces buried three decades ago. Additionally, samples Arthropoda species were investigated. In total, 150 strains and screened ability use plastic-related substrates (Impranil dispersions, polyethylene terephthalate, terephthalic acid, bis(2-hydroxyethyl) terephthalate). Twenty isolates selected based on their grow various identified Streptomyces, Bacillus, Enterococcus, Pseudomonas spp. Morphological features recorded, 16S rRNA sequence was employed construct phylogenetic tree. Subsequent assessments unveiled that 5 out 20 displayed capability produce polyhydroxyalkanoates, utilizing pre-treated post-consumer PET samples. With Priestia sp. DG69 Neobacillus DG40 emerging most successful producers (4.14% 3.34% PHA, respectively), these are poised further utilization in upcycling purposes, laying foundation development sustainable strategies waste management.

Language: Английский

Polyhydroxyalkanoates, the bioplastics of microbial origin: Properties, biochemical synthesis, and their applications DOI

Shivananda Behera,

Monika Priyadarshanee,

V.P. Vandana

et al.

Chemosphere, Journal Year: 2022, Volume and Issue: 294, P. 133723 - 133723

Published: Jan. 24, 2022

Language: Английский

Citations

75
A metagenomic perspective on the microbial prokaryotic genome census DOI Creative Commons
Dongying Wu, R. Seshadri, Nikos C. Kyrpides

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(3)

Published: Jan. 17, 2025

Following 30 years of sequencing, we assessed the phylogenetic diversity (PD) >1.5 million microbial genomes in public databases, including metagenome-assembled (MAGs) uncultivated microbes. As compared to vast uncovered by metagenomic sequences, cultivated taxa account for a modest portion overall diversity, 9.73% bacteria and 6.55% archaea, while MAGs contribute 48.54% 57.05%, respectively. Therefore, substantial fraction bacterial (41.73%) archaeal PD (36.39%) still lacks any genomic representation. This unrepresented manifests primarily at lower taxonomic ranks, exemplified 134,966 species identified 18,087 samples. Our study exposes hotspots freshwater, marine subsurface, sediment, soil, other environments, whereas human samples yielded minimal novelty within context existing datasets. These results offer roadmap future genome recovery efforts, delineating uncaptured underexplored environments underscoring necessity renewed isolation sequencing.

Language: Английский

Citations

5
The production, recovery, and valorization of polyhydroxybutyrate (PHB) based on circular bioeconomy DOI
Jianfei Wang, Jiaqi Huang, Shijie Liu

et al.

Biotechnology Advances, Journal Year: 2024, Volume and Issue: 72, P. 108340 - 108340

Published: March 26, 2024

Language: Английский

Citations

14
Inoculation with Bacillus megaterium CNPMS B119 and Bacillus subtilis CNPMS B2084 improve P-acquisition and maize yield in Brazil DOI Creative Commons

Christiane Abreu de Oliveira-Paiva,

Daniel Bini, S. M. de Sousa

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: June 26, 2024

Phosphorus (P) is a critical nutrient for plant growth, yet its uptake often hindered by soil factors like clay minerals and metal oxides such as aluminum (Al), iron (Fe), calcium (Ca), which bind P limit availability. Phosphate-solubilizing bacteria (PSB) have the unique ability to convert insoluble into soluble form, thereby fostering growth. This study aimed assess efficacy of inoculation Bacillus megaterium B119 (rhizospheric) B. subtilis B2084 (endophytic) via seed treatment in enhancing maize yield, grain content, enzyme activities across two distinct types field conditions. Additionally, we investigated various mechanisms contributing growth promotion, compatibility with commercial inoculants, root adhesion profile these strains. During five crop seasons experimental areas Brazil, Sete Lagoas-MG Santo Antônio de Goiás-GO, single inoculations either or were implemented three seasons, while co-inoculation both strains was applied seasons. All treatments received fertilizer according plot recommendations, except control. Both exhibited growth-promoting properties relevant dynamics, including phosphate solubilization mineralization, production indole-3-acetic acid (IAA)-like molecules, siderophores, exopolysaccharides (EPS), biofilms, phosphatases, no antagonism observed Azospirillum Bradyrizhobium . Strain displayed superior compared B119. In trials, resulted increased relative average productivities 22 16% Lagoas 6 3% Goiás, respectively. Co-inoculation proved more effective, an yield increase 24% 11% Goiás non-inoculated Across all accumulated content correlated availability rhizosphere after Goiás. These findings complement previous research efforts led validation registration first Brazilian inoculant formulated maize, effectively content.

Language: Английский

Citations

8
Heterologous expression of calcium-independent mesophilic α-amylase from Priestia megaterium: Immobilization on genipin-modified multi-walled carbon nanotubes and silica supports to enhance thermostability and catalytic activity DOI

Kübra Akbulut,

Sema Taranacı,

Sezen Özkök

et al.

Bioorganic Chemistry, Journal Year: 2025, Volume and Issue: 155, P. 108151 - 108151

Published: Jan. 8, 2025

Language: Английский

Citations

1
Unveiling novel features and phylogenomic assessment of indigenous Priestia megaterium AB-S79 using comparative genomics DOI Creative Commons
Adetomiwa A. Adeniji, Chinenyenwa Fortune Chukwuneme, Emilyn Costa Conceição

et al.

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

ABSTRACT Priestia megaterium strain AB-S79 isolated from active gold mine soil previously expressed in vitro heavy metal resistance and has a 5.7 Mb genome useful for biotechnological exploitation. This study used web-based bioinformatic resources to analyze P. genomic relatedness, decipher its secondary metabolite biosynthetic gene clusters (BGCs), better comprehend taxa. Genes were highly conserved across the 14 P . genomes examined here. The pangenome reflected total of 61,397 protein-coding genes, 59,745 homolog protein family hits, 1,652 singleton hits. There also 7,735 families, including 1,653 families 6,082 families. OrthoVenn3 comparison sequences with 13 other strains, 7 spp., 6 Bacillus spp. highlighted AB-S79’s unique evolutionary trait. antiSMASH identified two key transcription factor binding site regulators genome: zinc-responsive repressor (Zur) antibiotic production activator (AbrC3), plus putative enzymes biosynthesis terpenes ranthipeptides. harbors BGCs siderophores (synechobactins schizokinens), phosphonate, dienelactone hydrolase protein, phenazine (phzF), which is significant this study. Phosphonate particularly showed specificity sp. validating effect expansion contraction. looks be viable source value-added compounds. Thus, contributes theoretical framework systematic metabolic genetic exploitation sp., value-yielding strains. IMPORTANCE explores microbial natural product discovery using mining, focusing on Key findings highlight potential , AB-S79, applications. research shows limited output Africa, emphasizing importance native AB-S79. Additionally, underlines strain’s diverse capabilities, reinforcing suitability as model cell factories foundational role future

Language: Английский

Citations

1
Cell-free synthetic biology for natural product biosynthesis and discovery DOI Creative Commons
Andrew J. Rice,

Tien T. Sword,

Kameshwari Chengan

et al.

Chemical Society Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Cell free synthetic biology enables a broad range of approaches relevant to the generation, biosynthetic characterisation, bioengineering, activity profiling, and production complex natural products.

Language: Английский

Citations

1
A Plant Endophytic Bacterium Priestia megaterium StrainBP-R2 Isolated from the Halophyte Bolboschoenus planiculmis Enhances Plant Growth under Salt and Drought Stresses DOI Creative Commons
Hau‐Hsuan Hwang, Pei-Ru Chien,

Fan-Chen Huang

et al.

Microorganisms, Journal Year: 2022, Volume and Issue: 10(10), P. 2047 - 2047

Published: Oct. 17, 2022

Global warming and climate change have contributed to the rise of weather extremes. Severe drought soil salinization increase because rising temperatures. Economically important crop production plant growth development are hindered when facing various abiotic stresses. Plant endophytic bacteria live inside host plants without causing visible harm can be isolated from surface-sterilized tissues. Using stimulate environmental stress tolerance has become an alternative approach besides using traditional breeding genetically modifying approaches select or create new types resistant different The bacterium, Priestia megaterium (previously known as Bacillus megaterium) strain BP-R2, was root tissues salt marsh halophyte Bolboschoenus planiculmis. BP-R2 showed high sodium chloride (NaCl) concentrations produced auxin hormone, indole acetic acid (IAA), under tested conditions. Inoculation Arabidopsis pak choi (Brassica rapa L. R. Chinensis Group) with greatly enhanced parameters normal conditions compared that mock-inoculated plants. Furthermore, hydrogen peroxide (H2O2) content, electrolyte leakage (EL), malondialdehyde (MDA) concentration accumulated less in BP-R2-inoculated than control In summary, bacterium increased

Language: Английский

Citations

32
Consolidated bioprocessing of plant biomass to polyhydroxyalkanoate by co-culture of Streptomyces sp. SirexAA-E and Priestia megaterium DOI Creative Commons
Vijay Kumar, Brian G. Fox, Taichi E. Takasuka

et al.

Bioresource Technology, Journal Year: 2023, Volume and Issue: 376, P. 128934 - 128934

Published: March 20, 2023

Language: Английский

Citations

18
A Culturomics-Based Bacterial Synthetic Community for Improving Resilience towards Arsenic and Heavy Metals in the Nutraceutical Plant Mesembryanthemum crystallinum DOI Open Access
Noris J. Flores-Duarte, Eloísa Pajuelo, Enrique Mateos‐Naranjo

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(8), P. 7003 - 7003

Published: April 10, 2023

Plant-growth-promoting bacteria (PGPB) help plants thrive in polluted environments and increase crops yield using fewer inputs. Therefore, the design of tailored biofertilizers is utmost importance. The purpose this work was to test two different bacterial synthetic communities (SynComs) from microbiome Mesembryanthemum crystallinum, a moderate halophyte with cosmetic, pharmaceutical, nutraceutical applications. SynComs were composed specific metal-resistant plant-growth-promoting rhizobacteria endophytes. In addition, possibility modulating accumulation substances by synergetic effect metal stress inoculation selected tested. One isolated on standard tryptone soy agar (TSA), whereas other following culturomics approach. For that, culture medium based M. crystallinum biomass, called Mesem Agar (MA), elaborated. Bacteria three compartments (rhizosphere soil, root endophytes, shoot endophytes) TSA MA media, stablishing independent collections. All tested for PGP properties, secreted enzymatic activities, resistance towards As, Cd, Cu, Zn. best each collection order produce consortiums (denominated TSA- MA-SynComs, respectively), whose plant growth physiology, accumulation, metabolomics evaluated. Both SynComs, particularly MA, improved physiological parameters under mixture Regarding concentrations all metals/metalloids tissues below threshold toxicity, indicating that able soils when assisted metal/metalloid-resistant could be safely used pharmaceutical purposes. Initial analyses depict changes metabolome upon exposure inoculation, suggesting concentration high-value metabolites. usefulness both crop plant, namely Medicago sativa (alfalfa). results demonstrate effectiveness these alfalfa, improving growth, accumulation.

Language: Английский

Citations

18