Metabolite profiling of human‐originated Lachnospiraceae at the strain level

iMeta, Journal Year: 2022, Volume and Issue: 1(4)

Published: Oct. 13, 2022

Abstract The human gastrointestinal (GI) tract harbors diverse microbes, and the family Lachnospiraceae is one of most abundant widely occurring bacterial groups in GI tract. Beneficial adverse effects on host health were reported, but diversities at species/strain levels as well their metabolites have been, so far, not documented. In present study, we report collection 77 h uman‐originated L a ch nospiraceae sp ecies (please refer hLchsp, https://hgmb.nmdc.cn/subject/lachnospiraceae ) vitro metabolite profiles 110 strains ( https://hgmb.nmdc.cn/subject/lachnospiraceae/metabolites ). hLchsp produced 242 17 categories. larger categories alcohols (89), ketones (35), pyrazines (29), short (C2–C5), long (C > 5) chain acids (31), phenols (14), aldehydes other 30 compounds. Among them, 22 aromatic well‐known beneficial gut microbial metabolite, butyric acid, was generally by many strains, Agathobacter rectalis strain Lach‐101 Coprococcus comes NSJ‐173 top 2 acid producers, 331.5 310.9 mg/L vitro, respectively. Further analysis publicly available cohort‐based volatile‐metabolomic data sets feces revealed that over 30% prevailing volatile covered identified this study. This study provides resources together with metabolic for future studies host–microbe interactions developments novel probiotics or biotherapies.

Language: Английский

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Application of Metabolomics in Fungal Research

Molecules, Journal Year: 2022, Volume and Issue: 27(21), P. 7365 - 7365

Published: Oct. 29, 2022

Metabolomics is an essential method to study the dynamic changes of metabolic networks and products using modern analytical techniques, as well reveal life phenomena their inherent laws. Currently, more attention has been paid development histochemistry in fungus field. This paper reviews application metabolomics fungal research from five aspects: identification, response stress, metabolite discovery, metabolism engineering, interactions with plants.

Language: Английский

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Antifungal efficacy of Bacillus amyloliquefaciens ZK-9 against Fusarium graminearum and analysis of the potential mechanism of its lipopeptides

International Journal of Food Microbiology, Journal Year: 2024, Volume and Issue: 422, P. 110821 - 110821

Published: July 1, 2024

Language: Английский

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Current Strategies in Controlling Aspergillus flavus and Aflatoxins in Grains during Storage: A Review

Sustainability, Journal Year: 2024, Volume and Issue: 16(8), P. 3171 - 3171

Published: April 10, 2024

Aspergillus flavus is a ubiquitous pathogen that can infect many foods and grains, it produces large amounts of aflatoxins during their storage. Aflatoxins are considered highly toxic carcinogenic to humans, they cause great damage crop production, food security, human health. Thus, controlling A. in grains presents challenge humans worldwide. Over the past decade, strategies have been demonstrated be useful These methods involve physical agents, chemical biological etc. Some these currently used actual production. In this review, we summarize reported for storage ten years elucidate advantages disadvantages. The discussed include irradiation technology; low oxygen atmospheres; fungicides (benzalkonium chloride, iodine, ammonium bicarbonate, phenolic azole compounds); agents from plants, animals, micro-organisms; aflatoxin elimination methods. We expect review will promote applications current development novel technologies prevent or eliminate feed

Language: Английский

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The antifungal mechanisms of plant volatile compound 1-octanol against Aspergillus flavus growth

Applied Microbiology and Biotechnology, Journal Year: 2022, Volume and Issue: 106(13-16), P. 5179 - 5196

Published: July 2, 2022

Language: Английский

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Antifungal effects of trans-anethole, the main constituent of Illicium verum fruit volatiles, on Aspergillus flavus in stored wheat

Food Control, Journal Year: 2023, Volume and Issue: 149, P. 109721 - 109721

Published: March 1, 2023

Language: Английский

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Terpinen-4-ol from tea tree oil prevents Aspergillus flavus growth in postharvest wheat grain

International Journal of Food Microbiology, Journal Year: 2024, Volume and Issue: 418, P. 110741 - 110741

Published: May 9, 2024

Language: Английский

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Linalool, the main volatile constituent from Zanthoxylum schinifolium pericarp, prevents growth of Aspergillus flavus in post-harvest grains

Food Control, Journal Year: 2022, Volume and Issue: 137, P. 108967 - 108967

Published: March 16, 2022

Language: Английский

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Synergistic effects of ultrasound and plasma-activated water against Listeria innocua in crayfish disinfection by metabolomics analysis

Food Bioscience, Journal Year: 2024, Volume and Issue: 61, P. 104597 - 104597

Published: June 18, 2024

Language: Английский

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Gas Chromatography–Mass Spectrometry Profiling of Volatile Metabolites Produced by Some Bacillus spp. and Evaluation of Their Antibacterial and Antibiotic Activities

Molecules, Journal Year: 2023, Volume and Issue: 28(22), P. 7556 - 7556

Published: Nov. 12, 2023

Bacillus species produce different classes of antimicrobial and antioxidant substances: peptides or proteins with structural compositions molecular masses a broad range volatile organic compounds (VOCs), some which may serve as biomarkers for microorganism identification. The aim this study is the identification biologically active synthesized by five using gas chromatography coupled to mass spectrometry (GC-MS). current profoundly enhances knowledge antibacterial metabolites ensuring unambiguous VOCs produced species, were isolated from vegetable samples potato, carrot, tomato. Phylogenetic biochemical studies used identify bacterial isolates after culturing. analysis proved that BSS12, BSS13, BSS16, BSS21, BSS25 showed 99% nucleotide sequence similarities safensis AS-08, cereus WAB2133, acidiproducens NiuFun, toyonesis FORT 102, thuringiensis F3, respectively. crude extract was prepared assess antibiotic resistance potency potential against various targeted multidrug-resistant strains, including yeast strains such Candida albicans, krusei, Enterococcus hirae, Escherichia coli, Klebsiella aerogenes, pneumoniae, Staphylococcus aureus, epidermidis, Streptococcus group B, mutans, Shigella sonnei, Salmonella enteritidis, Serratia marcescens, Pseudomonas aeruginosa, Proteus vulgaris. GC-MS found come in variety chemical forms, ketones, alcohols, terpenoids, alkenes, etc. Overall, 69 identified all share components, have pharmacological applications. However, eight concentrations commonly species: acetoin, acetic acid, butanoic 2-methyl-, oxime-, methoxy-phenyl, phenol, 1,2-benzenedicarboxylic bis(2-methylpropyl) ester, nonanoic hexadecanoic methyl. present has demonstrated BSS25, BSS16 display potent inhibitory effects while BSS13 exhibit ability restrain growth activity krusei. Notably, BSS21 are only demonstrate substantial aerogenes. This disparity could be attributed higher acetoin whereas relatively elevated levels 2-methyl-. Certainly, presence contributes enhanced these conjunction other peptides, among factors. biology physiology can better understood results, also create novel biotechnological procedures Moreover, because its exceptional synthesize compounds, natural universal carriers form probiotic cultures fight pathogens, mycobacteria.

Language: Английский

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