Optimization of Fermentation Conditions and Product Identification of a Saponin-Producing Endophytic Fungus DOI Creative Commons
Qiqi Chen, Jingying Wang,

Yuhang Gao

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(9), P. 2331 - 2331

Published: Sept. 16, 2023

Some fungal endophytes isolated from P. ginseng may present a new method of obtaining saponins. This experiment aimed to optimize the total saponin yield produced through in vitro fermentation by an endophytic fungus and analyze its species fermented extract.Fermentation protocols were optimized with uniform design verified regression analysis maximize yield. The types under optimal conditions then identified analyzed using Liquid Chromatography-Mass Spectrometry.The Trametes versicolor strain NSJ105 (gene accession number: OR144428) wild could produce be increased more than two-fold optimization conditions. concentration saponins achieved protocol 105-DP was close predicted value. as follows: potato 97.3 mg/mL, glucose 20.6 inoculum volume 2.1%, broth pH 2.1, temperature 29.2 °C, time 6 d. It detected that extract contained ginsenosides Rf Rb3.The has potential application value production.

Language: Английский

Transcription factor PgNAC72 activates DAMMARENEDIOL SYNTHASE expression to promote ginseng saponin biosynthesis DOI
Ting Jiang, Yue Zhang, Gege Zuo

et al.

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 195(4), P. 2952 - 2969

Published: April 12, 2024

Abstract Ginsenosides, the primary bioactive constituents in ginseng (Panax ginseng), possess substantial pharmacological potential and are high demand market. The plant hormone methyl jasmonate (MeJA) effectively elicits ginsenoside biosynthesis P. ginseng, though regulatory mechanism remains largely unexplored. NAC transcription factors critical intricate networks participate numerous physiological activities. In this study, we identified a MeJA-responsive factor gene, PgNAC72, from transcriptome library produced MeJA-treated callus. Predominantly expressed flowers, PgNAC72 localizes to nucleus. Overexpressing (OE-PgNAC72) callus notably elevated total saponin levels, particularly dammarane-type ginsenosides, by upregulating dammarenediol synthase (PgDDS), encoding key enzyme pathway. Electrophoretic mobility shift assays dual-luciferase confirmed that binds NAC-binding elements PgDDS promoter, thereby activating its transcription. Further RNA-seq terpenoid metabolomic data OE-PgNAC72 line enhances biosynthesis. These findings uncover role of MeJA-mediated biosynthesis, providing insights into network presenting valuable target gene for metabolic engineering.

Language: Английский

Citations

14
Progress in Identification of UDP-Glycosyltransferases for Ginsenoside Biosynthesis DOI

Xiaoxuan Yuan,

Ruiqiong Li,

Weishen He

et al.

Journal of Natural Products, Journal Year: 2024, Volume and Issue: 87(4), P. 1246 - 1267

Published: March 7, 2024

Ginsenosides, the primary pharmacologically active constituents of

Language: Английский

Citations

11
The microbial biosynthesis of noncanonical terpenoids DOI Creative Commons

Mengyu Ma,

Mingkai Li,

Zhenke Wu

et al.

Applied Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 108(1)

Published: Feb. 21, 2024

Abstract Terpenoids are a class of structurally complex, naturally occurring compounds found predominantly in plant, animal, and microorganism secondary metabolites. Classical terpenoids typically have carbon atoms multiples five follow well-defined skeletons, whereas noncanonical deviate from these patterns. These often result the methyltransferase-catalyzed methylation modification substrate units, leading to irregular skeletons. In this comprehensive review, various activities applications terpenes been summarized. Importantly, review delves into biosynthetic pathways terpenes, including those with C6, C7, C11, C12, C16 bacteria fungi host. It also covers triterpenes synthesized non-squalene substrates nortriterpenes Ganoderma lucidum , providing detailed examples elucidate intricate processes involved. Finally, outlines potential future terpenoids. conclusion, insights gathered provide reference for understanding biosynthesis pave way discovery additional unique novel terpenes. Key points • The introduced . skeletons presented microbial is summarized

Language: Английский

Citations

9
Advancements in enzymatic biotransformation and bioactivities of rare ginsenosides：A Review DOI

Hu Yanbo,

Yiming Li,

Yi Cao

et al.

Journal of Biotechnology, Journal Year: 2024, Volume and Issue: 392, P. 78 - 89

Published: June 28, 2024

Language: Английский

Citations

9
Methyl jasmonate induces the regulation of protostane triterpene biosynthesis by microRNAs in Alisma orientale DOI

Wenyuan Run,

Tao Li, Shengyuan Wang

et al.

PROTOPLASMA, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Language: Английский

Citations

1
Structural Insights into the Substrate Recognition of Ginsenoside Glycosyltransferase Pq3‐O‐UGT2 DOI Creative Commons
Qiushuang Ji, Yirong Liu, Huanyu Zhang

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Abstract Ginsenosides are a group of tetracyclic triterpenoids with promising health benefits, consisting ginseng aglycone attached to various glycans. Pq3‐O‐UGT2, an important UDP‐dependent glycosyltransferase (UGT), catalyzes the production Ginsenoside Rg3 and Rd by extending glycan chain Rh2 F2, respectively, higher selectivity for F2. However, mechanism underlying its substrate recognition remains unclear. In this study, crystal structures Pq3‐O‐UGT2 in complex acceptor substrates solved. The revealed Nα5‐oriented binding pocket shaped unique conformation Nα5‐Nα6 linker. Hydrophobic interactions play pivotal role both while hydrogen bonds specifically aid F2 due additional glucose moiety. hydrophobic nature also enables recognize flavonoids. Overall, study provides novel insights into mechanisms ginsenoside UGTs, advancing understanding their function specificity.

Language: Английский

Citations

1
Ginsenosides for therapeutically targeting inflammation through modulation of oxidative stress DOI Creative Commons
Seungwha Paik, Gyu‐Yong Song, Eun‐Kyeong Jo

et al.

International Immunopharmacology, Journal Year: 2023, Volume and Issue: 121, P. 110461 - 110461

Published: June 16, 2023

Ginsenosides are steroid glycosides derived from ginseng plants such as Panax ginseng, quinquefolium, and notoginseng. Advances in recent studies have identified numerous physiological functions of each type ginsenoside, i.e., immunomodulatory, antioxidative, anti-inflammatory functions, the context inflammatory diseases. Accumulating evidence has revealed molecular mechanisms by which single or combined ginsenoside(s) exhibit effects, although it remains largely unclear. It is well known that excessive production reactive oxygen species (ROS) associated with pathological inflammation cell death a variety cells, inhibition ROS generation ameliorates local systemic responses. The ginsenosides attenuate unknown; however, targeting suggested one crucial for to control immune non-immune cells. This review will summarize latest progress ginsenoside studies, particularly antioxidant its effects. A better understanding distinct types action pave way developing potential preventive therapeutic modalities treating various inflammation-related

Language: Английский

Citations

19
A systematic review of ginsenoside biosynthesis, spatiotemporal distribution, and response to biotic and abiotic factors in American ginseng DOI
Lixia Tian, Ranran Gao, Yuxiang Cai

et al.

Food & Function, Journal Year: 2024, Volume and Issue: 15(5), P. 2343 - 2365

Published: Jan. 1, 2024

American ginseng ( Panax quinquefolius ) has gained recognition as a medicinal and functional food homologous product with several pharmaceutical, nutritional, industrial applications.

Language: Английский

Citations

7
Transcriptomic Analysis Reveals the Flavonoid Biosynthesis Pathway Involved in Rhizome Development in Polygonatum cyrtonema Hua DOI Creative Commons
Kui Wan,

Jingjie Ban,

Fengjie Yang

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(11), P. 1524 - 1524

Published: May 31, 2024

Polygonatum cyrtonema Hua (P. cyrtonema) rhizomes are rich in flavonoids and other secondary metabolites, exhibiting remarkable antioxidant, anti-tumor, immunomodulatory effects. flavonoid-biosynthesis-related genes have been characterized already. However, a comprehensive overview of flavonoid biosynthesis pathways is still absent. To articulate the accumulation pathways, we examined transcriptome changes using Illumina HiSeq from five different tissues RNA-seq 15 samples had over 105 Gb clean base, generating total 277,955 unigenes. The cDNA libraries fruits (F), leaves (L), roots (R), stems (S), (T) three-year-old P. plants generated 57,591, 53,578, 60,321, 51,530, 54,935 Comparative analysis revealed that 379 differentially expressed (DEGs) were group F _vs_ T, L R S transcripts DEGs principally enriched rhizomes. In addition, combined with WGCNA FPKM tissues’ transcription, nine transcription factor families (MYB, WRKY, AP2/ERF, etc.) red module, module positively correlated rhizome accumulation. Quantitative real-time PCR (qRT-PCR) further indicated BZIP1, C3H31, ERF114, DREB21 rhizomes, accompanied development cyrtonema. Therefore, this study provides foundation for research into uncovering

Language: Английский

Citations

6
Exploring the allelopathic autotoxicity mechanism of ginsenosides accumulation under ginseng decomposition based on integrated analysis of transcriptomics and metabolomics DOI Creative Commons
Rui Wang, Tingting Zhou, Yikai Wang

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: March 7, 2024

Continuous cropping obstacles seriously constrained the sustainable development of ginseng industry. The allelopathic autotoxicity ginsenosides is key “trigger” continuous in ginseng. During harvest, plants could be broken and remain soil. decomposition residue soil one important release ways ginsenosides. Therefore, mechanism through decomposed pathway needs an in-depth study. To investigate this regulation mechanism, integrated analysis transcriptomics metabolomics was applied. prototype were detected converse to rare during decomposition. caused more serious damage hairy root cells inhibited growth roots significantly. By high-throughput RNA sequencing gene study, significantly differential expressed genes (DEGs) obtained under ginsenoside interventions. These DEGs mainly enriched biosynthesis secondary metabolites metabolic pathways, phytohormone signal transduction, protein processing endoplasmic reticulum pathways. Based on functional enrichment DEGs, targeted based UPLC-MS/MS determination applied screen endogenous metabolized phytohormones (DMPs). influence accumulation studied. involved diterpenoid, zeatin, metabolites, After integrating analysis, regulate signaling pathways JA, ABA, SA. conclusion that converted into by released soil, which aggravated its autotoxicity. intervene response related This result provides a reference for study

Language: Английский

Citations

6