Unveiling the new chapter in nanobody engineering: advances in traditional construction and AI-driven optimization

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 6, 2025

Nanobodies (Nbs), miniature antibodies consisting solely of the variable region heavy chains, exhibit unique properties such as small size, high stability, and strong specificity, making them highly promising for disease diagnosis treatment. The engineering production Nbs has evolved into a mature process, involving library construction, screening, expression purification. Different types, including immune, naïve, synthetic/semi-synthetic libraries, offer diverse options various applications, while display platforms like phage display, cell surface non-surface provide efficient screening target Nbs. Recent advancements in artificial intelligence (AI) have opened new avenues Nb engineering. AI's exceptional performance protein structure prediction molecular interaction simulation introduced novel perspectives tools design optimization. Integrating AI with traditional experimental methods is anticipated to enhance efficiency precision development, expediting transition from basic research clinical applications. This review comprehensively examines latest progress engineering, emphasizing construction strategies, platform technologies, It evaluates strengths weaknesses libraries explores potential challenges predicting structure, antigen-antibody interactions, optimizing physicochemical properties.

Language: Английский

---

Recent advances in the application of CRISPR/Cas-based gene editing technology in Filamentous Fungi

Biotechnology Advances, Journal Year: 2025, Volume and Issue: 81, P. 108561 - 108561

Published: March 12, 2025

Language: Английский

---

Microbial production and applications of β-glucosidase-A review

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 256, P. 127915 - 127915

Published: Nov. 7, 2023

Language: Английский

---

Synthetic Biology Tools for Engineering Aspergillus oryzae

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(1), P. 34 - 34

Published: Jan. 3, 2024

For more than a thousand years,

Language: Английский

---

CRISPR/Cas9 mediated targeted knock-in of eglA gene to improve endoglucanase activity of Aspergillus fumigatus LMB-35Aa

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 23, 2024

Bioeconomy goals for using biomass feedstock biofuels and bio-based production has arisen the demand fungal strains enzymes processing. Despite well-known Trichoderma Aspergillus commercial strains, continuous bioprospecting revealed biodiversity potential of degrading enzymes. The strain fumigatus LMB-35Aa a great as source lignocellulose-degrading Nevertheless, genetic improvement should be considered to increase its biotechnological potential. Molecular manipulation based on homologous direct recombination (HDR) in filamentous fungi poses challenge since low rate. Currently, CRISPR/Cas9-mediated mutagenesis can enable precise efficient editing genomes. In this study, gene strategy improving endoglucanase activity A. was successfully used, which constitutes first report heterologous cellulase technology. For this, eglA from niger ATCC 10,864 integrated into conidial melanin pksP locus, facilitated selection edited events discerned by emergence albino colonies. Heterologous EglA enzyme biofilm fermentation system resulted 40% mutant compared wild type.

Language: Английский

---

Heterochromatin Protein Activates the Amylase Expression Pathway and Its Application to Recombinant Protein Expression in Penicillium oxalicum

Current Microbiology, Journal Year: 2025, Volume and Issue: 82(2)

Published: Jan. 9, 2025

Language: Английский

---

Biopharmaceuticals from filamentous fungi

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 253 - 290

Published: Jan. 1, 2025

Language: Английский

---

Insights into the morphology‐productivity relationship of filamentous fungi through small‐scale cultivation and automated microscopy of Thermothelomyces thermophilus

Biotechnology Progress, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Abstract Filamentous fungi are a cornerstone in the biotechnological production of enzymes, proteins, and organic acids. However, challenges understanding controlling relationship between morphology productivity can limit their application. This study addresses these using Thermothelomyces thermophilus , promising thermophilic fungus known for thermostable enzymes. We investigated effects environmental conditions on fungal enzyme combination microbioreactor cultivation, automated liquid handling, microscopy. Specifically, batch fed cultivations were performed at different pH levels glucose feeding rates to secretory phytase production, growth, morphology. Results from revealed two‐fold higher activity 5.5 compared 6.5, with notably smaller fragments end cultivation. Conversely, rate 1 g (l h) −1 showed 1.6‐fold 5.5, accompanied by much larger aggregates throughout phase. These findings suggest that large associated high productivity; however, breakdown further enhances release, increasing supernatant. not only provides insights morphology‐productivity T. but also demonstrates efficacy integrating microbioreactors methodology represents significant advance field biotechnology, paving way more efficient industrial bioprocesses.

Language: Английский

---

Filamentous fungi in biotechnology

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 221 - 232

Published: Jan. 1, 2025

Language: Английский

---

Applications of recombinant proteins in cultured meat production

Future Foods, Journal Year: 2025, Volume and Issue: 11, P. 100582 - 100582

Published: Feb. 20, 2025

Language: Английский

---