Frontiers in Cellular and Infection Microbiology,
Journal Year:
2024,
Volume and Issue:
14
Published: Oct. 16, 2024
Salmonella
enterica
ser.
Typhimurium
is
a
common
pathogen
that
poses
considerable
public
health
threat,
contributing
to
severe
gastrointestinal
diseases
and
widespread
foodborne
illnesses.
The
virulence
of
S.
regulated
by
quorum
sensing
(QS)
the
type
III
secretion
system
(T3SS).
This
study
investigated
inhibitory
effects
anti-QS
activity
epigallocatechin
gallate
(EGCG),
which
bioactive
ingredient
found
in
green
tea,
on
Typhimurium.
In
vitro
bacterial
experiments
demonstrated
EGCG
inhibited
production
autoinducers,
biofilm
formation,
flagellar
downregulating
expression
AI-1,
AI-2,
pathogenicity
islands
(SPI)-1,
SPI-2,
genes
related
flagella,
fimbriae,
curli
fibers.
mouse
model
Typhimurium-induced
enteritis,
considerably
reduced
intestinal
colonization
S
.
alleviated
damage.
conclusion,
protects
intestines
mice
infected
with
inhibiting
QS-induced
gene
expression,
demonstrating
its
potential
as
therapeutic
agent
for
controlling
infections.
Microorganisms,
Journal Year:
2024,
Volume and Issue:
12(5), P. 985 - 985
Published: May 14, 2024
Pseudomonas
aeruginosa
is
an
opportunistic
pathogen
that
usually
causes
chronic
infections
and
even
death
in
patients.
The
treatment
of
P.
infection
has
become
more
challenging
due
to
the
prevalence
antibiotic
resistance
slow
pace
new
development.
Therefore,
it
essential
explore
non-antibiotic
methods.
A
strategy
involves
screening
for
drugs
target
quorum-sensing
(QS)
system.
QS
system
regulates
drug
aeruginosa.
In
this
study,
veratryl
alcohol
(VA)
was
found
as
effective
inhibitor
(QSI).
It
effectively
suppressed
expression
QS-related
genes
subsequent
production
virulence
factors
under
control
including
elastase,
protease,
pyocyanin
rhamnolipid
at
sub-inhibitory
concentrations.
addition,
motility
activity
biofilm
formation,
which
were
correlated
with
aeruginosa,
also
by
VA.
vivo
experiments
demonstrated
VA
could
weaken
pathogenicity
Chinese
cabbage,
Drosophila
melanogaster,
Caenorhabditis
elegans
models.
Molecular
docking,
combined
quintuple
mutant
analysis,
identified
mechanism
LasR
protein
las
mainly.
Moreover,
increased
susceptibility
conventional
antibiotics
tobramycin,
kanamycin
gentamicin.
results
firstly
demonstrate
a
promising
QSI
treat
caused
Frontiers in Cellular and Infection Microbiology,
Journal Year:
2024,
Volume and Issue:
14
Published: Oct. 16, 2024
Salmonella
enterica
ser.
Typhimurium
is
a
common
pathogen
that
poses
considerable
public
health
threat,
contributing
to
severe
gastrointestinal
diseases
and
widespread
foodborne
illnesses.
The
virulence
of
S.
regulated
by
quorum
sensing
(QS)
the
type
III
secretion
system
(T3SS).
This
study
investigated
inhibitory
effects
anti-QS
activity
epigallocatechin
gallate
(EGCG),
which
bioactive
ingredient
found
in
green
tea,
on
Typhimurium.
In
vitro
bacterial
experiments
demonstrated
EGCG
inhibited
production
autoinducers,
biofilm
formation,
flagellar
downregulating
expression
AI-1,
AI-2,
pathogenicity
islands
(SPI)-1,
SPI-2,
genes
related
flagella,
fimbriae,
curli
fibers.
mouse
model
Typhimurium-induced
enteritis,
considerably
reduced
intestinal
colonization
S
.
alleviated
damage.
conclusion,
protects
intestines
mice
infected
with
inhibiting
QS-induced
gene
expression,
demonstrating
its
potential
as
therapeutic
agent
for
controlling
infections.
