Ethanol and 2-phenylethanol production by bee-isolated Meyerozyma caribbica strains DOI
Eduardo Dias Fenner, Stéfany Kell Bressan, Ângela Alves dos Santos

et al.

Preparative Biochemistry & Biotechnology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 11

Published: Oct. 10, 2024

Investigating the biotechnological potential of wild microorganisms is paramount for optimizing bioprocesses. Given this premise, we looked yeasts in Brazilian native stingless bees, considering recognized pollinating insect-associated production volatile organic compounds (VOCs). Two yeast strains species Meyerozyma caribbica were isolated from bees Scaptotrigona postica and evaluated their fermentative capacity. Both capable fermenting sucrose (the main sugar used ethanol industry) with over 90% efficiency yields up to 0.504 g/g. Through an experimental design analysis (CCD), it was verified that productivity these can also benefit high concentrations low pH values, desirable traits biofuel production. At same time, CCD analyses showed great capacity M. produce another alcohol broad interest, 2-phenylethanol. Interestingly, statistical demonstrated greater compound occur at availability nitrogen sources, which be easily achieved using residual low-cost feedstocks. Thus, our results suggest may efficiently both 2-phenylethanol

Language: Английский

Agro-ecosystem of honeybees as source for native probiotic yeasts DOI Creative Commons

Alice Agarbati,

Laura Moretti,

Laura Canonico

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 40(5)

Published: March 28, 2024

Abstract Probiotic microorganisms are used to improve the health and wellness of people research on this topic is current relevance interest. Fifty-five yeasts, coming from honeybee’s ecosystem belonging Candida , Debaryomyces Hanseniaspora Lachancea Metschnikowia Meyerozyma Starmerella Zygosacchromyces genera related different species, were evaluated for probiotic traits. The resistance gastrointestinal conditions, auto-aggregation, cell surface hydrophobicity or biofilm formation abilities as well antimicrobial activity against common human pathogenic bacteria evaluated. safety analysis strains was also carried out exclude any possible negative effect consumer’s health. influence proteinase treatment living yeasts their adhesion Caco-2 cells greatest selection occurred in first step survival at acidic pH presence bile salts, where more than 50% unable survive. Equally discriminating protease test which allowed only 27 species guilliermondii uvarum pulcherrima ziziphicola caribbica Pichia kluyveri kudriavzevii terricola . An integrated results obtained detection seven yeast with aptitudes, all genus, three M. guillermondii four M species.

Language: Английский

Citations

3
DNA in honey could describe the changes in flower visits and microbe encounters of honey bees over decades DOI Creative Commons
Alyssa R. Cirtwill, Helena Wirta

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 14, 2025

Abstract Recent environmental changes due to land-use and climate change threaten biodiversity the ecosystem services it provides. Understanding true scope of these is complicated by lack historical baselines for many interactions underpinning services, such as pollination, or disservices, disease spreading. To assess in vital find ways comparing past current between species. Here, we focus on honey bees – one world’s most important agricultural pollinators, plants they visit, microbes encounter environment. DNA offers insights into contemporary bees. Old samples could serve describe bees’ previous decades, providing a baseline against which over time. By identifying taxonomic origin plant, bacterial fungal fifty-year-old samples, show that plant can reveal visited past. Likewise, microbe records microbes, including pollinator pathogens, encountered possibly spread. However, some differences recovered old new suggest degradation different bias naive comparisons samples. Like other types ancient may be useful historically occurred should not taken proof an interaction did occur. Keeping limits data mind, time series offer unique information about how associations with flowers have changed during decades change.

Language: Английский

Citations

0
New fungal core microbiome members of the ground nesting bee Andrena vaga: The key to oligolecty? DOI Creative Commons
Hanna Gardein, Silvio Erler, Henri Greil

et al.

Basic and Applied Ecology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
First Report on Antifungal Activity of Metschnikowia pulcherrima Against Ascosphaera apis, the Causative Agent of Chalkbrood Disease in Honeybee (Apis mellifera L.) Colonies DOI Creative Commons
Massimo Iorizzo, Francesca Coppola, Gianfranco Pannella

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(5), P. 336 - 336

Published: April 25, 2025

Chalkbrood is the manifestation of fungal disease caused by Ascosphaera apis, which affects broods developing honeybees, particularly in Apis mellifera colonies. Recently, Metschnikowia pulcherrima has been proposed as a biocontrol agent winemaking and for management major postharvest soil-borne plant pathogenic fungi. In this study, antagonistic activity three M. strains against fifteen A. apis strains, isolated from contaminated hives mellifera, was evaluated, with specific focus on potential antifungal volatile organic compounds (VOCs). The study revealed that effective related to various mechanisms including competition nutrients, secretion pulcherriminic acid, biosynthesis VOCs. We also found each strain produced unique combination VOCs, strain-dependent varied depending yeast-mold combination. addition, preliminary analyses showed temperature 30 °C higher amount glucose (40 g/L) growing medium promote growth apis. These results could be useful designing new strategies chalkbrood honeybee

Language: Английский

Citations

0
Diversity of Culturable Yeasts in the Feces of Mew Gulls Breeding in Natural and Urban Habitats, with Insights into the Antifungal Susceptibility of the Observed Pathogens DOI Creative Commons
А. М. Глушакова, А. В. Качалкин

Birds, Journal Year: 2024, Volume and Issue: 5(3), P. 543 - 557

Published: Aug. 22, 2024

Migratory birds play an important role in the spread of yeasts environment over long distances and different geographical regions. Human activities, turn, have a major impact on biology wild and, consequently, microbial communities for which act as carriers disseminators. We sought to assess “response” diversity culturable feces Mew Gulls type nesting site (natural/anthropogenic) during breeding season from April October 2023. isolated molecularly identified 26 yeast species. The species composition natural habitat was more diverse, increased October. In contrast, urban decreased Analysis susceptibility conventional antibiotics (fluconazole, voriconazole, amphotericin B) using CLSI BMD (Clinical Laboratory Standards Institute broth microdilution) method strains opportunistic Candida (C. parapsilosis C. tropicalis) emerging pathogen Rhodotorula mucilaginosa showed that proportion resistant higher anthropogenic population. spent their near landfill flew away wintering appear be source pathogenic with resistance against antifungal agents.

Language: Английский

Citations

2
Ethanol and 2-phenylethanol production by bee-isolated Meyerozyma caribbica strains DOI
Eduardo Dias Fenner, Stéfany Kell Bressan, Ângela Alves dos Santos

et al.

Preparative Biochemistry & Biotechnology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 11

Published: Oct. 10, 2024

Investigating the biotechnological potential of wild microorganisms is paramount for optimizing bioprocesses. Given this premise, we looked yeasts in Brazilian native stingless bees, considering recognized pollinating insect-associated production volatile organic compounds (VOCs). Two yeast strains species Meyerozyma caribbica were isolated from bees Scaptotrigona postica and evaluated their fermentative capacity. Both capable fermenting sucrose (the main sugar used ethanol industry) with over 90% efficiency yields up to 0.504 g/g. Through an experimental design analysis (CCD), it was verified that productivity these can also benefit high concentrations low pH values, desirable traits biofuel production. At same time, CCD analyses showed great capacity M. produce another alcohol broad interest, 2-phenylethanol. Interestingly, statistical demonstrated greater compound occur at availability nitrogen sources, which be easily achieved using residual low-cost feedstocks. Thus, our results suggest may efficiently both 2-phenylethanol

Language: Английский

Citations

0