ROLE OF INTERCELLULAR ADHESION MOLECULES (ICAM-1), VASCULAR CELL ADHESION (VCAM-1) AND CALPROTECTIN (MRP8/14) IN PATHOGENESIS OF DIABETIC RETINOPATHY DOI Creative Commons
О. A. Saklakova,

M. V. Maksimenya,

Е. В. Фефелова

et al.

Zabajkalʹskij medicinskij vestnik, Journal Year: 2024, Volume and Issue: 2, P. 52 - 60

Published: July 23, 2024

Aim of the research . The aim is to study content intercellular adhesion molecules (ICAM-1), vascular cell molecule (VCAM-1) and calprotectin in blood serum patients with type 2 diabetes mellitus various stages diabetic retinopathy. also evaluate role these pathogenesis disease. Materials methods Four groups people were formed: first group (control group) included 21 healthy individuals; second prediabetes, third diabetes. fourth 63 retinopathy, this was further divided into 3 each: non-proliferative stage DR, preproliferative stage, proliferative stage.. concentrations ICAM-1, VCAM-1 (MRP8/14) determined using Human Vascular Inflammation Panel 1 multiplex analysis kits from Biolegend (USA). results assessed CytoFlex flow cytometer calculated Jamovi version 2.3. Results. In individuals MRP8/14 increased by 111,7% (p < 0,001) relative control group. without values protein exceed 2,7 times those prediabetes 29,2% = 0,049). who had levels are higher than complications. During number increases even more; during remain high, level ICAM-1 previous stages. Conclusion. Increasing increasing initial DR demonstrate initiation Researching relationship between markers development can provide additional information develop strategies for prevention treatment as well predicting its

Language: Английский

Advances in Vascular Diagnostics using Magnetic Particle Imaging (MPI) for Blood Circulation Assessment DOI
Marisa O. Pacheco,

Isabelle K Gerzenshtein,

Whitney L. Stoppel

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: June 16, 2024

Abstract Rapid and accurate assessment of conditions characterized by altered blood flow, cardiac pooling, or internal bleeding is crucial for diagnosing treating various clinical conditions. While widely used imaging modalities such as magnetic resonance (MRI), computed tomography (CT), ultrasound offer unique diagnostic advantages, they fall short specific indications due to limited penetration depth prolonged acquisition times. Magnetic particle (MPI), an emerging tracer‐based technique, holds promise circulation assessments, potentially overcoming existing limitations with reduction in background signals high temporal spatial resolution, below the millimeter scale. Successful pooling impaired flow necessitates tracers diverse half‐lives optimized MPI signal generation. Recent show potential cardiovascular complications, vascular perforations, ischemia, stroke. The impressive resolution also position excellent modality real‐time vessel perfusion via functional (fMPI). This review summarizes advancements analyzes current state pre‐clinical applications. work discusses perspectives on standardization required transition from a research endeavor implementation explore additional that may benefit capabilities MPI.

Language: Английский

Citations

4
Comment on “Pathogenesis-Guided Rational Engineering of Nanotherapies for the Targeted Treatment of Abdominal Aortic Aneurysm by Inhibiting Neutrophilic Inflammation” DOI Creative Commons
Heng Wang, Keyi Fan,

Xiaohua Jia

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: 19(2), P. 1861 - 1864

Published: Jan. 21, 2025

InfoMetricsFiguresRef. ACS NanoVol 19/Issue 2Article This publication is free to access through this site. Learn More CiteCitationCitation and abstractCitation referencesMore citation options ShareShare onFacebookX (Twitter)WeChatLinkedInRedditEmailJump toExpandCollapse Letter the EditorJanuary 21, 2025Comment on "Pathogenesis-Guided Rational Engineering of Nanotherapies for Targeted Treatment Abdominal Aortic Aneurysm by Inhibiting Neutrophilic Inflammation"Click copy article linkArticle link copied!Heng WangHeng WangCentre Transplant Renal Research, Westmead Institute Medical The University Sydney, New South Wales 2145, AustraliaMore Heng Wanghttps://orcid.org/0000-0001-7408-0398Keyi FanKeyi FanDepartment Vascular Surgery, Second Hospital Shanxi University, Taiyuan, 030001, ChinaMore Keyi FanXiaohua JiaXiaohua JiaKey Laboratory Molecular Imaging Chinese Academy Sciences, Automation, Beijing 100190, Xiaohua JiaRuijing Zhang*Ruijing ZhangDepartment Nephrology, China*Email: [email protected]More Ruijing ZhangHonglin Dong*Honglin DongDepartment Honglin DongGuoping Zheng*Guoping ZhengCentre Australia*Email: Guoping ZhengOpen PDFACS NanoCite this: Nano 2025, 19, 2, 1861–1864Click citationCitation copied!https://pubs.acs.org/doi/10.1021/acsnano.4c12263https://doi.org/10.1021/acsnano.4c12263Published January 2025 Publication History Received 2 September 2024Accepted 2025Revised 22 November 2024Published online 21 2025Published in issue 2025letterCopyright © Published American Chemical Society. available under these Terms Use. Request reuse permissionsThis licensed personal use PublicationsCopyright SocietySubjectswhat are subjectsArticle subjects automatically applied from Subject Taxonomy describe scientific concepts themes article.CellsImmunologyInflammationNanoparticlesRodent modelsRecently, Hu et al. (1) designed a nanotherapy targeting aortic abdominal aneurysm (AAA) inhibiting neutrophil inflammation. approach based lumino-conjugated α-cyclodextrin materials (LaCD), which anti-inflammatory nanoparticles (LaCD NPs) were synthesized. capability nanoprobe neutrophils was also demonstrated their previous study. (2) Additionally, modifying LaCD NPs with alendronate sodium, (AlaCD obtained, significantly enhanced ability target calcification within aneurysm. modification thereby inhibits neutrophil-mediated inflammatory responses aneurysm, particularly proinflammatory effects extracellular traps (NETs), matrix degradation, promotion vascular smooth muscle cell apoptosis.A Brief Overview Nanotherapeutics AAAClick section linkSection copied!Nanotherapeutics AAA represent an emerging trend treatment human disease. Nanomaterials possess unique properties such as high surface area, small size effects, potential, making them suitable drug delivery, (3) imaging guidance, (4) tissue repair. These help achieve therapeutic concentrations at lesion sites, that difficult conventional methods. Various nanomedicines have shown potential animal models (Table 1).Table 1. Examples Nanomedicine Delivery AAADiseaseNanoparticlesTargetsEffectsAAA, Rats (5)Rapamycin-loaded PEG-b-PBLGBeing endocytosed macrophagesThe level inhibitedAAA, (6)Cation functionalized PLGA-PANPFibrin clot intraluminal thrombusThe intracavitary thrombus dissolvedAAA, Mice (3)EVMS@RGD-HBc NCMacrophages cells expression αvβ3Inhibition macrophage polarization phenotypic switchingAAA, (7)EL-BSA-NP-PGGDamaged elastinInhibition recruitmentAAA, (8)Nanoparticles vesicles (EVs) derived mesenchymal stem (MSC)Immune cellsReduced inflammation activationAAA, (20)Supramolecular nanofibers using peptide amphiphile moleculesFragmented elastin, metalloproteinase (MMP-2), membrane type 1 metalloproteinaseOnly targeted localizationAAA, (21)TPN-siRNA, formed oxidative polymerization self-assembly epigallocatechin gallateMacrophages cellsA siRNA released silence MMP-2 MMP-9, promote M1 M2 repolarization macrophages, inhibit apoptosisAAA, (22)A rapamycin-loaded oxidation-responsive β-cyclodextrin materialIntegrins membranesIt attenuated infiltration CD68 macrophages outer rupture media rat AAA, reduced calcification, inhibited elastin degradationNanomedicine can be passively lesions or taken up specific cells, leveraging structure injured artery. For example, PEG-b-PBLG loaded rapamycin accumulate microdefects reducing being macrophages. (5) Another example PLGA-PANP, targets fibrin clots, helping reduce arterial injury. (6)Active involves conjugating ligands attaching drug-loaded cells. Fandi Mo developed EVMS@RGD-HBc NC viral actively αvβ3 coordinating microenvironment. Nasim Nosoudi constructed EL-BSA-NP-PGG nanoframe carriers damaged elastin-targeting particles recruitment tissues calcium chloride rats. (7)Nanoparticles internalized hitchhiking effect, responding signals sites delivery. Michael Spinosa discovered (MSC) -derived could modulate response progression via miR-147. (8)Several studies highlight advantages nanoparticles, sustained release lower concentrations. demonstrate efficacy protecting elastic fibers, though further research needed clarify immunogenicity, stability, bioavailability clinical translation. while nanomaterial-based photothermal therapy used cancer treatment, it remains unexplored AAA.Limitations StudyClick copied!To our knowledge, first study address therapy, paving way precision other diseases. has following limitations require clarification.1.Myeloperoxidase (MPO) not sufficiently precise marker neutrophils. MPO protein believed secreted both diseases, atherosclerosis, positively correlated plaque vulnerability. (9,10) To certain extent, reasonable choose state reflect activity However, legend Figure S1B accurate, would more rigorously represented markers Ly6G.2.Improving probe's vivo imaging. noted "due deep location aorta abdomen low accumulation Cy5/LaCD aortas relative major organs.″ Consequently, subsequent studies, they abandoned favor ex fluorescence (see original S5, 2F). Observation only visualize distribution characteristics nanomaterials but reveal changes metabolism. Using different strategies improve physical chemical fluorescent probes understand physiological processes diagnosis, prognosis We suggest near-infrared dyes Cy7 IRDye 800CW enhance penetration. (11,12) coupling Fe3O4 magnetic resonance nanoparticle overcome depth (13) may worthwhile explore mice, dorsal imaging, fasting dehydration protocols. (14) Developing integrated diagnostic platform significant implications.3.The fate monocytes peripheral blood. proposed "LaCD bloodstream, followed hitchhiking-effect-mediated translocation aneurysmal due cells." Figures 5F-J S18 indicate exhibit decreased migratory capacity, factor release, diminished recruit treatment. Therefore, after intravenous injection, immune metabolized degraded, resulting limited quantity reaching In future authors attempt elucidate interaction between carrier On one hand, cultured vitro viability migration engulfing examined relation dose time administration. then infused back into mice vivo, real-time elucidation quantification vector-cell viability. isolated animals, aggregation residual should detected times expect confirm will critical translation.4.Hu conducted RNA sequencing suggested close relationship activation. technique cannot ascertain cellular origin MPO. Performing single-cell purified yield results.5.Utilize common models. Among existing models, widely used. (15) Methods adventitial application porcine pancreatic elastase, CaCl2 solution infusion, angiotensin II slow-release pump implantation commonly (16−18) agree choosing experimental model; larger essential transition humans, especially context applications new technologies, including nanoparticles. no single modeling method fully true complexity AAA. Employing multiple acute chronic aneurysms provide accurate representation various scenarios pathogenesis. It closer pathological crucial mechanism pathophysiology identification targets. look forward validation excellent performance studies.6.Additionally, there some minor errors 3F 3G, intimal integrity content stained EVG 50 mg/kg group do appear show improvement. S9, dye Cy5 rather than Cy3. S11, appears deposition indicated alizarin red staining shows insufficient structural integrity. Quantitative analysis objectively clearly support results. addition, we replace representative images, readers effect intuitively.The purpose letter offer relevant knowledge suggestions improvement, affect results conclusions al.'s hope researchers additional approaches disease mortality rupture. Currently, preoperative detection practice primarily relies morphological techniques computed tomography angiography (CTA) ultrasound (19) presented precision-targeted strategy diseases immunological perspective, offering translational potential.Finally, like acknowledge extensive work thank designing delivery system aimed improving therapy.Author InformationClick copied!Corresponding AuthorsRuijing Zhang - Department China; Email: protected]Honglin Dong protected]Guoping Zheng Centre Australia; protected]AuthorsHeng Wang https://orcid.org/0000-0001-7408-0398Keyi Fan ChinaXiaohua Jia Key ChinaAuthor ContributionsThe manuscript written contributions all authors. All given approval final version manuscript.FundingThis supported NHMRC Ideas grant 2027965 Translational Medicine Research Center Diseases Province, China (Grant No. 2022017).AbbreviationsClick copied!AAAaortic aneurysmLaCD NPslumino-conjugated nanoparticlesNETsneutrophil trapsMSCmesenchymal cellEVsextracellular vesiclesMPOmyeloperoxidaseCTAcomputed angiographyReferencesClick copied! references publications. 1Hu, K.; Zhong, L.; Lin, W.; Zhao, G.; Pu, Feng, Z.; Zhou, M.; Ding, J.; Zhang, J. Pathogenesis-Guided Inflammation. 2024, 18 (8), 6650– 6672, DOI: 10.1021/acsnano.4c00120 Google ScholarThere corresponding record reference.2Tao, H.; Guo, Ma, Y.; Jin, T.; Gu, Dou, Liu, Hu, Xiong, X.; Luminescence Acute Liver Injury Biodegradable Biocompatible Nanoprobes. 2020, 14 (9), 11083– 11099, 10.1021/acsnano.0c00539 Scholar2Luminescence NanoprobesTao, Hui; Jiawei; Yongchang; Yang; Taotao; Lijuan; Yin; Jinyi; Houyuan; Xiaoxing; JianxiangACS (2020), 11083-11099CODEN: ANCAC3; ISSN:1936-0851. (American Society) injury result hepatic fatty liver, liver fibrosis, hepatitis, failure, mainly responsible global morbidity. Early diagnosis crit. Herein report luminescence injury, alc. (ALI) failure (ALF). purpose, biodegradable luminescent material chem. functionalization cyclic oligosaccharide, produced nanoprobes (defined NPs). dependent reactive oxygen species myeloperoxidase (MPO). Correspondingly, activated specifically imaged NPs, signal pos. assocd. count. mouse ALI ALF, enabled tracking livers. cases, intensity consistent time-dependent profiles neutrophils, MPO, parameters pathogenesis Moreover, capacity addnl. improved neutrophil-targeting peptide. addn., preliminary good safety NPs. effective biocompatible dynamic development promising neutrophil-assocd. >> SciFinder ®https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3cXhsFOisb%252FN&md5=46acf3fc91a4bf907428cfae74a9f1fa3Mo, F.; Wang, C.; Li, S.; Xiao, E.; P.; Yuan, Zuo, Fu, Chen, Ren, L. A Dual-Targeting, Multi-Faceted Nanodrug Optimizes Microenvironment Ameliorate Aneurysm. Advanced (Deerfield Beach, Fla.) 36 (33), e2405761 10.1002/adma.202405761 reference.4Wang, R.; Jia, Gao, Fan, Qiao, Yan, Hui, Dong, H. Highly sensitive particle NETosis anti-Ly6G iron oxide Cell death discovery 10 (1), 395, 10.1038/s41420-024-02156-3 reference.5Shirasu, Koyama, Miura, Hoshina, Kataoka, Watanabe, T. Nanoparticles Effectively Target Rapamycin Sites Experimental Rats. PloS 2016, 11 (6), e0157813 10.1371/journal.pone.0157813 reference.6Sivaraman, B.; Sylvester, A.; Ramamurthi, A. Fibrinolytic PLGA slow lysis attenuate proteolytic loss matrix. Materials science & engineering. C, biological 59, 145– 156, 10.1016/j.msec.2015.09.056 reference.7Nosoudi, N.; Chowdhury, Siclari, Parasaram, V.; Karamched, Vyavahare, N. Systemic Loaded Pentagalloyl Glucose Protects Elastic Lamina Prevents Journal cardiovascular 9 (5–6), 445– 455, 10.1007/s12265-016-9709-x reference.8Spinosa, Lu, Su, Bontha, S. Gehrau, Salmon, M. D.; Smith, Weiss, Mas, V. Upchurch, G. R., Jr.; Sharma, K. Human stromal cell-derived formation activation microRNA-147. FASEB journal: official Federation Societies Biology 2018, 32 (11), 6038 10.1096/fj.201701138RR reference.9Rashid, I.; Maghzal, Y. Cheng, Talib, Newington, Vajandar, Searle, Maluenda, Lindstedt, E. Jabbour, Kettle, Bongers, Power, Michaelsson, Peter, Stocker, R. Myeloperoxidase molecular stabilization high-risk atherosclerotic plaque. Eur. Heart 39 (35), 3301– 3310, 10.1093/eurheartj/ehy419 Scholar9Myeloperoxidase plaqueRashid, Imran; Ghassan Yung-Chih; David; Jihan; Darren; Minqin; Saumitra Amy; Ana; Eva-Lotte; Andrew; Antony Andre; Carl; Erik; Karlheinz; RolandEuropean (2018), 3301-3310CODEN: EHJODF; ISSN:1522-9645. (Oxford Press) As enzyme abundant ruptured plaques, investigate role employed tandem stenosis model instability apolipoprotein E gene knockout (Apoe-/-) mice. test Mpo-/-Apoe-/- 2-thioxanthine inhibitor AZM198. assessed liq. chromatog.-tandem mass spectrometry 2-chloroethidium generation hydroethidine bis-5HT-DTPA-Gd (MPO-Gd) mol. (MRI), phenotype verified histol. two-fold greater unstable compared stable phenotype. Genetic deletion increased fibrous cap thickness, haemosiderin AZM198 thickness phenotype, without affecting blood circulating leukocytes lipids. MPO-Gd MRI enhancement T1-weighted Mpo gene. Our data implicate non-invasive pharmacol. inhibition hold promise clin. translation management coronary artery ®https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC1MXhtlKju7bK&md5=287250d7c0704abde344944ca95d2ac110Sugiyama, Okada, Sukhova, Virmani, Heinecke, Libby, P. Macrophage regulation granulocyte colony-stimulating atherosclerosis implications syndromes. Am. Pathol. 2001, 158 (3), 879– 91, 10.1016/S0002-9440(10)64036-9 Scholar10Macrophage syndromesSugiyama, Seigo; Yoshikatsu; Galina Renu; Jay PeterAmerican Pathology (2001), 879-891CODEN: AJPAA4; ISSN:0002-9440. Society Investigative Pathology) Inflammation stress contribute many atherosclerosis. atheroma contains levels produces pro-oxidant species, hypochlorous acid (HOCl). documents nos. myeloperoxidase-expressing eroded plaques causing contrast, streaks contain little myeloperoxidase. Granulocyte factor, selectively regulates express produce HOCl vitro. myeloperoxidase-pos. co-localized factor. Pro-inflammatory stimuli known present plaque, CD40 ligand, lysophosphatidylcholine, cholesterol crystals, induce prodn. HOCl-modified proteins accumulated atheroma. identify endogenous regulator particular complication ®https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BD3MXitlOnurY%253D&md5=ef265d90189152083a67c4a96cb6be9f11Tong, Shang, Tian, Q.; Yang, vulnerable active myeloperoxidase-targeted Theranostics 2021, (2), 506– 521, 10.7150/thno.49812 reference.12Zhang, Liang, Zhu, Du, Xue, Optical multimodality plectin-1-targeted agent orthotopic ductal adenocarcinoma EBioMedicine 2022, 80, 104040 10.1016/j.ebiom.2022.104040 Scholar12Optical miceZhang, Wenjia; Xiaolong; Liang; Xinyu; Zhengyu; Jie; HuadanEBioMedicine (2022), 80 (), 104040CODEN: EBIOAX; ISSN:2352-3964. (Elsevier B.V.) Pancreatic (PDAC) lethal malignancy worldwide, challenging currently. Magnetic (MPI) internal PDAC tumors because its sensitivity unlimited depth. utilize MPI, combination (FMI) advance xenografts. plectin-1 IRDye800CW conjugated superparamagnetic (PTP-Fe3O4-IRDye800CW) PDAC-targeting triple-modality S.c. established. FMI, performed quant. observation tumors. Histol. analyses validation. PTP-Fe3O4-IRDye800CW possessed great expressed multi-modality higher specificity, even distribution, longer retention over 7 d Con-Fe3O4-IRDye800CW (MPI, 2d post-injection: PTP-Fe3O4-IRDye800CW: 85.72% ± 1.53% vs. Con-Fe3O4-IRDye800CW: 74.41% 1.91%, **P < 0.01 (Student's t test)). Ex Prussian blue stainings validate probes. demonst

Language: Английский

Citations

0
Kirenol ameliorates endotoxin-induced acute lung injury by inhibiting the ERK and JNK phosphorylation–mediated NFκB pathway in mice DOI
Frank Cheau‐Feng Lin, Shih‐Pin Chen, Sheng‐Di Lin

et al.

Inflammopharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Language: Английский

Citations

0
Exploring the diagnostic potential: magnetic particle imaging for brain diseases DOI Creative Commons

Lishuang Guo,

Yu An,

Ze-Yu Zhang

et al.

Military Medical Research, Journal Year: 2025, Volume and Issue: 12(1)

Published: April 27, 2025

Abstract Brain diseases are characterized by high incidence, disability, and mortality rates. Their elusive nature poses a significant challenge for early diagnosis. Magnetic particle imaging (MPI) is novel technique with sensitivity, temporal resolution, no ionizing radiation. It relies on the nonlinear magnetization response of superparamagnetic iron oxide nanoparticles (SPIONs), allowing visualization spatial concentration distribution SPIONs in biological tissues. MPI expected to become mainstream technology diagnosis brain diseases, such as cancerous, cerebrovascular, neurodegenerative, inflammatory diseases. This review provides an overview principles MPI, explores its potential applications discusses prospects management these

Language: Английский

Citations

0
Molecular imaging: The bridge from human phenome to personalized precision medicine DOI Creative Commons
Mei Tian, Yidan Gao,

Chenxi Xue

et al.

European Journal of Nuclear Medicine and Molecular Imaging, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 26, 2024

Language: Английский

Citations

1
A greedy regularized block Kaczmarz method for accelerating reconstruction in magnetic particle imaging DOI
Yusong Shen, Liwen Zhang, Hui Zhang

et al.

Physics in Medicine and Biology, Journal Year: 2024, Volume and Issue: 69(15), P. 155004 - 155004

Published: June 11, 2024

Magnetic particle imaging (MPI) is an emerging medical tomographic modality that enables real-time with high sensitivity and spatial temporal resolution. For the system matrix reconstruction method, MPI problem ill-posed inverse commonly solved using Kaczmarz algorithm. However, computation time of algorithm, which restricts speed, has limited development potential clinical applications for MPI. In order to achieve fast in MPI, we propose a greedy regularized block method (GRBK) accelerates reconstruction.

Language: Английский

Citations

0
ROLE OF INTERCELLULAR ADHESION MOLECULES (ICAM-1), VASCULAR CELL ADHESION (VCAM-1) AND CALPROTECTIN (MRP8/14) IN PATHOGENESIS OF DIABETIC RETINOPATHY DOI Creative Commons
О. A. Saklakova,

M. V. Maksimenya,

Е. В. Фефелова

et al.

Zabajkalʹskij medicinskij vestnik, Journal Year: 2024, Volume and Issue: 2, P. 52 - 60

Published: July 23, 2024

Aim of the research . The aim is to study content intercellular adhesion molecules (ICAM-1), vascular cell molecule (VCAM-1) and calprotectin in blood serum patients with type 2 diabetes mellitus various stages diabetic retinopathy. also evaluate role these pathogenesis disease. Materials methods Four groups people were formed: first group (control group) included 21 healthy individuals; second prediabetes, third diabetes. fourth 63 retinopathy, this was further divided into 3 each: non-proliferative stage DR, preproliferative stage, proliferative stage.. concentrations ICAM-1, VCAM-1 (MRP8/14) determined using Human Vascular Inflammation Panel 1 multiplex analysis kits from Biolegend (USA). results assessed CytoFlex flow cytometer calculated Jamovi version 2.3. Results. In individuals MRP8/14 increased by 111,7% (p < 0,001) relative control group. without values protein exceed 2,7 times those prediabetes 29,2% = 0,049). who had levels are higher than complications. During number increases even more; during remain high, level ICAM-1 previous stages. Conclusion. Increasing increasing initial DR demonstrate initiation Researching relationship between markers development can provide additional information develop strategies for prevention treatment as well predicting its

Language: Английский

Citations

0