Improving the nutritional value and bioactivity of soybean meal in solid-state fermentation using Bacillus strains newly isolated from the gut of the termite Termes propinquus

FEMS Microbiology Letters, Journal Year: 2022, Volume and Issue: 369(1)

Published: Jan. 1, 2022

The present study aimed to isolate and characterize proteolytic Bacillus spp. from termite guts test the possibility of application for improving nutritional value bioactivity fermented soybean meal (FSBM). Aerobic endospore-forming bacteria were isolated gut Termes propinquus. Ten isolates with high levels soy milk degradation selected tested extracellular enzyme production. Among them, two isolates, Tp-5 Tp-7, exhibited all hydrolytic activities (cellulase, xylanase, pectinase, amylase, protease, lipase phytase), weak alpha hemolytic also antagonistic against fish pathogenic species Aeromonas Streptococcus. Both phylogenetic biochemical analyses indicated that they closely related amyloliquefaciens. During solid-state fermentation SBM, Tp-7 highest protease activity (1127.2 1552.4 U g-1, respectively) at 36 h, resulting FSBMs showed a significant increase in crude protein content free radical-scavenging ability (P < 0.05), as well an improvement composition amino acids, metabolites other nutrients, while indigestible materials such fiber, lignin hemicellulose decreased. potential strains, especially improved FSBM by their strong antioxidant activities, together reducing antinutritional components.

Language: Английский

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Contrasting responses of cuticular bacteria of Pardosa pseudoannulata under cadmium stress

Ecotoxicology and Environmental Safety, Journal Year: 2023, Volume and Issue: 255, P. 114832 - 114832

Published: March 28, 2023

Although research into how spiders respond to cadmium (Cd)-induced toxicity is ongoing, little known about the effects of Cd contamination on exogenous microorganisms spiders. The current study used 16 S rRNA gene sequencing evaluate richness and structure external bacterial communities in wolf spider Pardosa pseudoannulata under long- short-term stress. Our results showed that Proteobacteria Acidibacter were dominating phylum genus. alpha diversity analysis high background concentration (Cd) reduced diversity, (SCd) stress elevated diversity. Hub genera, including Stenotrophobacter, Hymenobacter, Chitinophaga, Bryobacter, identified by co-occurrence network connectance with other genera. Further investigation revealed 15 14 taxa classified distinctively SCd stresses. Interestingly, functional prediction enhanced some crucial pathways involved specialized functions, manganese oxidation aromatic compound degradation. Random forest correlation analyses found spider's molting time was dominant driver affecting phyla (i.e., Planctomycetes) genera (e.g., Acidibacter, Reyranella, Haliangium). Collectively, this comprehensive creates new perspectives investigate divergent responses microbial spiders' habitat stress, provides valuable viewpoints for pollution evaluation control.

Language: Английский

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Identification of Acetomycin as an Antifungal Agent Produced by Termite Gut-Associated Streptomycetes against Pyrrhoderma noxium

Antibiotics, Journal Year: 2024, Volume and Issue: 13(1), P. 45 - 45

Published: Jan. 3, 2024

Plant fungal pathogen Pyrrhoderma noxium is responsible for the destructive and invasive disease of brown root rot currently affecting city Brisbane, Australia. In order to address this issue, environmentally friendly safe alternatives chemical control are preferred due city’s public setting. Antifungal natural products ideal candidates as biological can be detected through investigating metabolomes microbial symbionts. Within study, an NMR-based metabolomics approach was applied fermentation extracts obtained from 15 termite gut-associated streptomycetes. By analysing NMR spectra, six which displayed similar profiles exhibited antifungal activity against P. pathogen. The major compound within these identified acetomycin using X-ray crystallography analyses. This first reporting a potential product fungicide, particularly agent noxium. Inhibitory also found other important crop pathogens, including Aspergillus niger, Botrytis cinerea, Alteranaria alternata. Further experimentation woodblock test inhibitory on growth up 3 weeks significant difference in integrity woodblocks when conducting compression strength tests after 6 weeks. Therefore, may used fungicide

Language: Английский

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Oxidative cleavage of cellulose by fungi in the termite gut

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 284, P. 138222 - 138222

Published: Nov. 30, 2024

Language: Английский

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A Comprehensive account of Functional Role of Insect Gut Microbiome in Insect Orders

Journal of Natural Pesticide Research, Journal Year: 2024, Volume and Issue: unknown, P. 100110 - 100110

Published: Dec. 1, 2024

Language: Английский

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