Frontiers in Marine Science,
Journal Year:
2022,
Volume and Issue:
9
Published: Feb. 28, 2022
Probiotic
use
in
aquaculture
settings
can
be
an
approach
for
disease
control
and
dietary
supplementation.
We
assessed
the
antagonistic
effect
of
culture
supernatants
lactic
acid
bacteria
on
growth
known
shrimp
pathogens,
Vibrio
(
Listonella
)
anguillarum
,
alginolyticus
V.
harveyi
using
a
quantitative
microplate
bioassay.
Supernatants
from
Lactobacillus
curvatus
subsp.
curvatus,
L.
plantarum
Pediococcus
acidolactici
significantly
inhibited
these
vibrios.
The
active
component(s)
were
heat
stable
(>
100°C)
resistant
to
freeze-thawing.
Most
this
inhibitory
activity
was
brought
about
by
production
pH;
however,
there
evidence
other
factors
playing
role.
In
search
novel
probiotic
bacteria,
organism
isolated
gastrointestinal
tract
healthy
whiteleg
Penaeus
vannamei)—
identified
tentatively
as
Carnobacterium
maltaromaticum
.
This
isolate,
had
less
potent
vibriocidal
than
reduced
survival
at
dose
1
×
10
7
bacteria/shrimp.
During
28-day
feeding
trial,
juvenile
P.
vannamei
fed
with
supplemented
diets
showed
no
gross
changes
parameters
compared
control.
suggest
that
could
incorporated
into
biofloc
formulations
purge
pathogenic
vibrios
pond
settings,
rather
being
directly
shrimp.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Feb. 28, 2024
Abstract
This
study
aimed
to
examine
the
distribution
of
anaerobic
bacteria
in
rumen
fluid
Thai
crossbred
goats
and
screen
potential
probiotic
strains
capable
producing
antimicrobial
compounds
inhibiting
that
cause
milk
fat
depression.
Thirty-four
from
were
divided
into
13
groups
within
12
genera
based
on
16S
rRNA
gene
sequences.
The
RF1-5
RF5-12
identified
as
Streptococcus
luteliensis
Bacillus
licheniformis
,
respectively,
demonstrated
non-ropy
exopolysaccharide.
Furthermore,
mPRGC5
T
was
closely
related
Selenomonas
caprae
JCM
33725
(97.8%
similarity)
It
exhibited
low
average
nucleotide
identity,
digital
DNA–DNA
hybridization,
amino
acid
identity
values
with
type
ranging
84.9
86.0%,
21.3
21.8%,
73.8
76.1%,
respectively.
genotypic
phenotypic
characteristics
strongly
support
this
strain
a
new
species
genus
for
which
name
ruminis
proposed.
is
(=
33724
=
KCTC
25177
).
Ligilactobacillus
salivarius
MP3
showed
antibacterial
activity
against
Cutibacterium
acnes
subsp.
DSM
1897
Kocuria
rhizophila
MIII.
enterolysin
A
cluster
its
genome.
auto-aggregation
L.
93.6
±
0.2%.
Additionally,
co-aggregation
C.
K.
MIII
had
92.2
3.4%
87.3
4.5%,
adhesion
capacity
76.11
2.2%.
Probiogenomic
analysis
revealed
nonhazardous
animal
supplementation
included
acid-
bile-tolerant
ability.
However,
contained
three
antibiotic
resistance
genes.
Thus,
could
increase
content
by
suppressing
horizontal
transfer
awareness.
Microbiology Spectrum,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 9, 2024
ABSTRACT
Eimeria
maxima
is
a
major
cause
of
coccidiosis
in
chickens
and
key
predisposing
factor
for
other
economically
significant
diseases
such
as
necrotic
enteritis.
However,
detailed
understanding
the
intestinal
microbiome
response
to
E.
infection
still
lacking.
This
study
aimed
comprehensively
investigate
dynamic
changes
14
days
post-infection
(dpi)
with
.
Bacterial
16S
rRNA
gene
sequencing
was
performed
ileal
cecal
digesta
collected
from
mock
maxima-
infected
at
prepatent
(3
dpi),
acute
(5
7
recovery
phases
(10
dpi)
infection.
Although
no
notable
were
observed
3
dpi,
alterations
microbiota
occurred
both
ileum
cecum
5
dpi.
By
tended
return
healthy
state.
Notably,
Lactobacillus
enriched
cecum,
although
individual
,
Ligilactobacillus
Limosilactobacillus
species
varied
temporal
pattern
response.
Concurrently,
short-chain
fatty
acid-producing
bacteria,
Faecalibacterium
progressively
suppressed
by
cecum.
On
hand,
opportunistic
pathogens
Escherichia
Enterococcus
Staphylococcus
significantly
during
IMPORTANCE
We
have
first
time
infection,
synchronized
its
life
cycle.
Minimal
occur
early
while
coincide
disruption
mucosal
lining.
As
animals
recover
coccidiosis,
largely
returns
normal.
-induced
inflammation
likely
creates
an
environment
conducive
growth
aerotolerant
anaerobes
well
facultative
suppressing
obligate
bacteria.
These
findings
expand
our
dynamics
structure
offer
insights
into
pathogenesis
supporting
rationale
microbiome-based
strategies
control
prevention
this
condition.
Frontiers in Antibiotics,
Journal Year:
2022,
Volume and Issue:
1
Published: Oct. 12, 2022
The
increasing
global
expansion
of
antimicrobial
resistant
infections
warrants
the
development
effective
antibiotic
alternative
therapies,
particularly
for
use
in
livestock
production,
an
agricultural
sector
that
is
perceived
to
disproportionately
contribute
resistance
(AMR)
crisis
by
consuming
nearly
two-thirds
supply.
Probiotics
and
probiotic
derived
compounds
are
promising
their
successful
disease
prevention,
treatment,
animal
performance
commands
attention.
However,
insufficient
or
outdated
screening
techniques
may
unintentionally
this
crisis,
few
longitudinal
studies
have
been
conducted
determine
what
role
probiotics
play
AMR
dissemination
hosts
surrounding
environment.
In
review,
we
briefly
summarize
current
literature
regarding
efficacy,
feasibility,
limitations
probiotics,
including
evaluation
impact
on
microbiome
resistome
potential
influence
Probiotic
application
often
touted
as
ideal
therapy
might
reduce
need
agriculture
negative
downstream
impacts.
detailed
limited
research
has
linking
usage
with
reductions
natural
environments.
Additionally,
discuss
methods,
limitations,
across
globe,
highlighting
approaches
aimed
at
reducing
ensuring
safe
mediated
health
outcomes.
Based
information,
propose
economic
logistical
considerations
bringing
therapies
market
regulatory
roadblocks,
future
innovations,
significant
gaps
knowledge
requiring
additional
ensure
suitable
long-term
options
producers
therapy.
Microorganisms,
Journal Year:
2025,
Volume and Issue:
13(1), P. 160 - 160
Published: Jan. 14, 2025
Lactobacillus
is
a
key
genus
of
probiotics
commonly
utilized
for
the
treatment
oral
infections
The
primary
aim
our
research
was
to
investigate
probiotic
potential
newly
isolated
Levilactobacillus
brevis
DPL5
strain
from
human
breast
milk,
focusing
on
its
ability
combat
biofilm-forming
pathogens
such
as
Staphylococcus
aureus.
Employing
in
vitro
approaches,
we
demonstrate
L.
DPL5′s
endure
at
pH
3
with
survival
rates
above
30%,
and
withstand
osmotic
stress
often
found
during
industrial
processes
like
fermentation
freeze
drying,
retaining
over
90%
viability.
lyophilized
cell-free
supernatant
had
significant
antagonistic
effect
against
biofilm-producing
nasal
strains
aureus,
it
completely
eradicated
biofilms
subinhibitory
concentrations
20
mg·mL−1.
Higher
69
mg·mL−1
were
have
99%
bactericidal
effect,
based
conducted
probability
analysis,
indicating
production
bioactive
extracellular
compounds
capable
disrupting
biofilm
formation
S.
Furthermore,
genome-wide
sequencing
analysis
cutting-edge
Nanopore
technology
has
uncovered
50
genes
linked
activity,
supporting
adapt
thrive
harsh
gut
environment.
genome
also
contains
multiple
biosynthetic
gene
clusters
lanthipeptide
class
IV,
Type
III
polyketide
synthase
(T3PKS),
ribosomally
synthesized,
post-translationally
modified
peptides
(RiPP-like
compounds),
all
which
are
associated
antibacterial
properties.
Our
study
paves
way
further
exploration
DPL5,
setting
stage
innovative,
nature-inspired
solutions
stubborn
bacterial
infections.
Pathogens,
Journal Year:
2025,
Volume and Issue:
14(1), P. 81 - 81
Published: Jan. 16, 2025
The
intestinal
microbiota
is
known
to
be
altered
by
Eimeria-induced
coccidiosis,
but
it
remains
unclear
whether
the
fully
restored
after
recovery.
To
address
this,
110
newly
hatched
Cobb
male
broiler
chickens
were
challenged
with
2
×
104
sporulated
oocysts
of
Eimeria
maxima
(EM)
strain
M6
or
mock-infected
saline
on
day
10.
Body
weight
and
feed
intake
recorded.
Additionally,
10
mock-
12
EM-infected
birds
randomly
selected
assess
small
lesion,
fecal
oocyst
shedding,
ileal
cecal
compositions
using
16S
rRNA
gene
sequencing
at
3,
5,
7,
14,
21
days
post-infection
(dpi).
EM
infection
significantly
decreased
(p
<
0.001)
body
5
dpi,
persisting
through
dpi.
also
reduced
0.05)
gain,
intake,
efficiency
in
first
week;
however,
these
parameters
became
comparable
second
third
weeks.
At
7
during
peak
infection,
major
lactic
acid
bacteria
enriched,
while
short-chain
fatty
acid-producing
mostly
suppressed
both
ileum
cecum.
Opportunistic
pathogens
such
as
Escherichia
Clostridium
perfringens
transiently
bloomed
By
14
differential
bacterial
enrichment
subsided,
nearly
all
commensal
returned
healthy
levels
Coupled
growth
performance
between
EM-recovered
chickens,
we
conclude
that
largely
its
state
Understanding
microbiota's
responses
coccidiosis
may
inform
probiotic-based
mitigation
strategies.
