RSC Advances,
Journal Year:
2025,
Volume and Issue:
15(8), P. 6050 - 6067
Published: Jan. 1, 2025
Several
fused/binary
pyrazole
quinolinone
hybrids
are
synthesized
via
tandem
reactions
and
their
insecticidal
efficacy
against
S.
littoralis
A.
gossypii
evaluated.
Physiologia Plantarum,
Journal Year:
2025,
Volume and Issue:
177(2)
Published: March 1, 2025
The
identification
and
functional
analysis
of
key
stress
tolerance-related
genes
are
paramount
importance
in
elucidating
mechanisms
regulating
plant
responses
adaptation
to
environmental
stresses.
Significant
progress
has
been
achieved
mining
through
the
integrative
metabolome
other
omics
data.
However,
methodologies
for
precise
secondary
metabolites
still
require
further
refinement.
This
study
introduces
a
novel
approach
discovering
critical
by
integrating
isolation
drought-responsive
transcriptome
(IISMTA).
Using
this
approach,
four
(RMs),
namely
formononetin,
afrormosin,
ononin,
wistin,
were
isolated
from
Onobrychis
viciifolia
characterized
chromatography,
high-resolution
mass
spectrometry
(HRMS)
nuclear
magnetic
resonance
(NMR)
technologies.
Only
formononetin
was
identified,
while
latter
three
undetected
widely-targeted
analysis,
indicating
novelty
our
approach.
A
correlational
between
proposed
biosynthetic
pathways
RMs
data
drought-stressed
vs.
non-stressed
O.
seedlings
conducted
identify
involved.
Among
upregulated
potentially
involved,
OvIF7GT,
encoding
an
isoflavone
glycosyltransferase,
ectopically
expressed
Arabidopsis
thaliana
assess
its
role
biosyntheses
these
compounds
drought
adaptation.
Results
indicated
that
OvIF7GT
transgenic
plants
showed
increased
total
flavonoid
contents
tolerance
associated
with
enhanced
antioxidant
defense
osmoprotection,
reduced
oxidative
damage.
Therefore,
IISMTA
developed
is
valuable
complement
existing
gene
metabolite
discovery
approaches.
BMC Genomics,
Journal Year:
2025,
Volume and Issue:
26(1)
Published: May 9, 2025
Heat
stress
seriously
affects
the
production
and
health
of
dairy
cows
is
a
key
factor
limiting
sustainable
development
industry.
DNA
methylation
serves
as
an
important
epigenetic
regulatory
mechanism
closely
associated
with
animal's
response
to
heat
stress.
However,
specific
molecular
in
cows'
not
fully
understood.
In
this
study,
whole
genome
bisulfite
sequencing
analysis
blood
identified
49861
differentially
methylated
regions
corresponding
7613
genes
between
spring
summer
cows.
Among
them,
4069
promoter
region
were
significantly
enriched
biological
pathways
such
substance
transport,
reactive
oxygen
species
metabolism,
signal
transduction,
energy
metabolism.
By
integrating
expression
data
genes,
157
further
screened,
their
levels
negatively
correlated
gene
expression.
The
changes
DNLZ,
GNAS,
SMAD5
most
significant,
network
showed
that
DNLZ
has
high
connectivity
protein-protein
interaction
network,
indicating
its
potential
function
response.
Experimental
verification
shows
under
conditions,
level
CpG
islands
increases,
level.
Dual-luciferase
reporter
assays
using
constructs
containing
experiment
confirms
inhibits
transcriptional
activity,
higher
degree
methylation,
stronger
inhibitory
effect.
research
results
provide
new
insights
into
stress-related
cows,
clarify
roles
target
markers
for
cultivation
heat-resistant
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(10), P. 4515 - 4515
Published: May 9, 2025
The
increasing
global
population
and
intensifying
resource
limitations
present
a
formidable
challenge
for
sustainable
crop
production,
especially
in
developing
regions.
This
review
explores
the
pivotal
role
of
microbial
ecosystem
services
alleviating
environmental
stresses
that
impede
agricultural
productivity.
Soil
microbiota,
particularly
plant
growth-promoting
microbes
(PGPMs),
are
integral
to
soil
health
fertility
resilience
against
both
abiotic
(drought,
salinity,
temperature
extremes,
heavy
metals)
biotic
(pathogen)
stresses.
These
microorganisms
employ
variety
direct
indirect
mechanisms,
including
modulation
phytohormones,
nutrient
solubilization,
production
stress-alleviating
enzymes,
synthesis
antimicrobial
compounds,
enhance
growth
mitigate
adverse
impacts.
Advances
biotechnology
have
expanded
toolkit
harnessing
beneficial
microbes,
enabling
development
inoculants
consortia
tailored
specific
stress
conditions.
highlights
multifaceted
contributions
such
as
improving
uptake,
promoting
root
development,
facilitating
pollutant
degradation,
supporting
carbon
sequestration,
all
which
underpin
practices.
Furthermore,
synergistic
interactions
between
roots
rhizospheric
emphasized
key
drivers
structure
enhancement
long-term
By
synthesizing
current
research
on
mechanisms
microbe-mediated
tolerance,
this
underscores
potential
interventions
bridge
gap
food
security
conservation.
integration
solutions
into
agroecosystems
offers
promising,
eco-friendly
strategy
revitalize
soils,
boost
yields,
ensure
sustainability
face
mounting
challenges.
