Biosynthesized ZnO-CuO Nanocomposite for Biofilm Formation of Proteus mirabilisupon LuxS Gene Expression

Inorganics, Journal Year: 2025, Volume and Issue: 13(2), P. 65 - 65

Published: Feb. 19, 2025

Proteus mirabilis has been identified as the third most frequent reason for catheter-associated urinary tract infections. The production of urease significantly enhances force catheter blockage caused by biofilm formation. Because biofilms are important virulence factors that make antibiotics less potent, it is becoming increasingly to develop novel alternative antibiotics. In addition unique properties they possess, nanoparticles made from metal oxide currently attracting considerable attention possible antibacterials. This research aims explore potential anti-biofilm green manufactured ZnO-CuO generated P. mirabilis. By synthesizing reductive enzymes, bacterial cells can participate in biosynthesis process. study explores whether synthesized work an agent formed These were using Bacteriocins determine their effectiveness against bacteria, which partially purified and showed antimicrobial activity Gram-negative bacteria AFM, TEM, FESEM, XRD, ultraviolet (UV)–visible spectroscopy used analyze biosynthesized ascertain chemical physical characteristics. XRD verified hexagonal structure, TEM demonstrated a size range 96.00 nm, FESEM surface morphology. dispersion roughness shown through AFM examination. produced nanoparticles’ UV-visible spectra displayed maximum peak at 287 232 nm. When applied strains (wild-type) (multidrug-resistant), copper zinc had notable inhibitory effects. Weak effectively generate biofilms, following incubation with 128 μg/mL subminimum concentrations (MICs) CuO 24 48 h 37 °C. Following treatment nanocomposite these strains, downregulation alterations LuxS expression detected utilizing real-time PCR After this, isolates treated nanocomposite, downregulated shifts found technique contrast not treated. Zinc (ZnO) be utilized antibacterial agents concentration-dependent manner, aligning all observed findings. present demonstrates oxide–zinc nanocomposites effective Their noteworthy gene successfully prevents formation swarming motility.

Language: Английский

Harnessing the functional role of polymeric nanocomposite in dental caries disease: A comprehensive review

Hybrid Advances, Journal Year: 2025, Volume and Issue: unknown, P. 100433 - 100433

Published: March 1, 2025

Language: Английский

Solvent-free synthesis and characterization of nanocurcumin-reinforced zinc oxide bio-nanocomposites for enhanced optoelectronic applications

Applied Physics A, Journal Year: 2025, Volume and Issue: 131(5)

Published: April 7, 2025

Language: Английский