Wide-Field Quantitative Ghost Phase Imaging with Phase-Shifting Holographic Ghost Diffraction DOI Creative Commons

R. V. Vinu,

Yanyan Huang,

Xingpeng Du

et al.

Optics Express, Journal Year: 2024, Volume and Issue: 33(1), P. 531 - 531

Published: Dec. 23, 2024

Ghost holography has attracted notable applied interest in the modern quantitative imaging applications with futuristic features of complex field recovery diversified scenarios. However, utilization digital ghost frame works introduces space bandwidth or time restrictions implementation technique domains. Here, we propose and demonstrate a phase approach holographic diffraction scheme combination phase-shifting technique. The makes use an off-axis system by superposing fields reference random generated from independent diffuser. In addition, utilizes high-speed response spatial light modulator to introduce fast temporal shifting one ghost-diffraction that views object, which practically results enhancement effective frequency domain suppressing redundant terms. applicability is experimentally validated demonstrating various abrupt continuous samples.

Language: Английский

Multi-modal transport of intensity diffraction tomography microscopy with an electrically tunable lens DOI Creative Commons
Zihao Zhou, Runnan Zhang, Ning Zhou

et al.

Biomedical Optics Express, Journal Year: 2025, Volume and Issue: 16(2), P. 837 - 837

Published: Jan. 15, 2025

Optical diffraction tomography (ODT) is an important technique for three-dimensional (3D) imaging of semi-transparent biological samples, enabling volumetric visualization living cells, cultures, and tissues without the need exogenous dyes. However, ODT faces significant challenges in complex specimens due to limited specificity refractive index (RI) coupled relationship between absorption phase image formation. Here, we present multi-modal transport intensity (MM-TIDT), a high-speed 3D microscopy that integrates electrically tunable lens with modified illumination patterns decouple information. Leveraging dual schemes-circular annular apertures-MM-TIDT acquires two stacks, facilitating accurate decoupling. Based on alternating direction method multipliers (ADMM) framework total variation (TV) non-negativity regularization, our reconstructs distributions fluorescence RI high accuracy robustness. Experimental validation fluorescent polystyrene microspheres, Spirulina specimens, DAPI-labeled C166 cells demonstrates capability MM-TIDT resolve fine structural details across diverse sample types, providing versatile platform exploring dynamic processes intricate cellular interactions.

Language: Английский

Citations

1

Hybrid Iterating-Averaging Low Photon Budget Gabor Holographic Microscopy DOI Creative Commons
Mikołaj Rogalski, Piotr Arcab, Emilia Wdowiak

et al.

ACS Photonics, Journal Year: 2025, Volume and Issue: 12(4), P. 1771 - 1782

Published: Jan. 10, 2025

Achieving high-contrast, label-free imaging with minimal impact on live cell culture behavior remains a primary challenge in quantitative phase (QPI). By enabling under low illumination intensities (low photon budget, LPB), it is possible to minimize photostimulation, phototoxicity, and photodamage while supporting long-term high-speed observations. However, LPB introduces significant difficulties QPI due high levels of camera shot noise quantification noise. Digital in-line holographic microscopy (DIHM) technique known for its robustness against data. simultaneous minimization inherent DIHM twin image perturbation critical challenge. In this study, we present the iterative Gabor averaging (IGA) algorithm, novel approach that integrates retrieval frame effectively suppress both disturbance multiframe DIHM. The IGA algorithm achieves by leveraging an process reconstructs high-fidelity images selectively across frames. Our simulations demonstrate consistently outperforms conventional methods, achieving superior reconstruction accuracy, particularly high-noise conditions. Experimental validations involving dynamic sperm cells static test target measurement further confirmed IGA's efficacy. also proved successful optically thin samples, which often yield signal-to-noise holograms even at budgets. These advancements make powerful tool photostimulation-free, biological samples enhance ability extremely optical thickness, potentially transforming biomedical environmental applications low-light settings.

Language: Английский

Citations

0

Portable Filter-Free Lens-Free Incoherent Digital Holography System DOI Creative Commons
Tatsuki Tahara

Photonics, Journal Year: 2025, Volume and Issue: 12(2), P. 167 - 167

Published: Feb. 19, 2025

A portable incoherent digital holography system without a polarization filter or refractive lens was developed. Phase-shifted self-interference holograms of light diffracted from an object were generated attenuation due to using two polarization-sensitive phase-only spatial modulators (TPP-SLMs). The number optical elements in filter-free lens-free reduced make the compact and portable. Experiments conducted developed set on tripod stand objects illuminated by light-emitting diode.

Language: Английский

Citations

0

Multi-Channel Cellytics for Rapid and Cost-Effective Monitoring of Leukocyte Activation DOI Creative Commons

Hye-Ran Cheon,

Samir Kumar, Inha Lee

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(3), P. 143 - 143

Published: Feb. 24, 2025

Morphological changes in leukocytes are valuable markers for diseases and immune responses. In our earlier work, we presented Cellytics, a device that uses lens-free shadow imaging technology (LSIT) to monitor natural killer cell activity. Here, present an improved Cellytics system has been upgraded four-channel configuration achieve higher throughput while maintaining robust reproducibility rapid cost-effective leukocyte analysis. The performance of this multi-channel was through refinements the micro-pinhole chip. Etched pinholes provided better image resolution clarity compared drilled pinholes. To stimulate leukocytes, used activation stimulator cocktail (ASC) quantified resulting morphological using shadow-based metrics, including peak-to-peak distance (PPD) maxima-to-minima standard deviation (MMD-SD). addition, developed new parameter (LAP) specifically assess these activation-induced changes. After ASC stimulation, showed significantly increased PPD LAP values decreased MMD-SD non-activated leukocytes. These results consistent with flow cytometric emphasize potential accurate assessment provide tool both clinical diagnostics basic immunological research.

Language: Английский

Citations

0

Quantitative Phase Imaging Based on the Coded Aperture Correlation Holography Cross‐Correlation Reconstruction Method DOI
Xuelian Yu,

ZhengXian Wang,

J. R. Niu

et al.

Laser & Photonics Review, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

Abstract Interference‐free coded aperture correlation holography (I‐COACH) is an innovative incoherent digital method capable of recording 3D scenes without lasers or dual‐wave interference, offering significant potential in diverse applications. quantitative phase imaging (QPI) a noninvasive optical technique for extracting information transparent samples, often requiring interference diffraction combined with reconstruction algorithms. However, I‐COACH's partially coherent illumination and noninterference limit its extraction capability. A complex amplitude cross‐correlation introduced to overcome these limitations. This avoids numerical inversion, distance considerations, mask (CPM) modulation effects. It reconstructs the sample's by determining amplitudes point source hologram (PSH) object (OH) applying cross‐correlation. Experimental results from I‐COACH confirm ability reconstruct distributions effectively. The also offers excellent refocusing remains applicable under illumination. approach expands utility I‐COACH, particularly biomedical imaging.

Language: Английский

Citations

0

Fourier Holo-Ptychographic Microscopy: A Hybrid Digital Holography-Fourier Ptychography Approach to Synthetic-Aperture Quantitative Phase Microscopy DOI Creative Commons
Zhuoshi Li, Yefeng Shu, Linpeng Lu

et al.

ACS Photonics, Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

Language: Английский

Citations

0

Metasurface-Refractive Hybrid Lens Modeling with Vector Field Physical Optics DOI Creative Commons
Ko-Han Shih, C. Kyle Renshaw

Photonics, Journal Year: 2025, Volume and Issue: 12(4), P. 401 - 401

Published: April 21, 2025

Metasurfaces (MSs) have emerged as a promising technology for optical system design. When combined with traditional refractive optics, MS-refractive hybrid lenses can enhance imaging performance, reduce aberrations, and introduce new functionalities such polarization control. However, modeling these requires advanced simulation techniques that usually go beyond conventional raytracing tools. This work presents physical optics framework lenses. We ray-wave method integrates multiple propagation to account vector wave through various elements. At the center of proposed is Gaussian decomposition beam optics. Ray-path diffraction automatically considered in this method, complex input wavefront be modeled well. Several are integrated ensure accuracy decomposing an incoming into beamlets, adaptive consideration local principal curvatures best-fit width estimation from covariance matrix. To demonstrate effectiveness our we apply it several lens designs, including polarization-sensitive MSs aberration-correcting systems.

Language: Английский

Citations

0

Propagation of autofocusing Airy vortex beams with quadratic phase modulation DOI
Qian Zhang, Qixuan Chen, Haolong He

et al.

Optics & Laser Technology, Journal Year: 2025, Volume and Issue: 187, P. 112759 - 112759

Published: March 16, 2025

Language: Английский

Citations

0

Long-Range Imaging through Scattering Media Using Deep Learning DOI Creative Commons
Ying Jin, Cangtao Zhou,

Wanjun Dai

et al.

Photonics, Journal Year: 2024, Volume and Issue: 11(9), P. 887 - 887

Published: Sept. 20, 2024

Imaging through scattering media is an important and challenging problem, the technology has been used in many fields, such as autonomous driving, industrial inspections, remote sensing imaging, biomedical imaging. However, most of previous experiments numbers or letters for close-range while objects life are colorful. In this study, a new deep learning network, DesUNet, was constructed to image realistic at medium long distances under sunlight media, realize object recognition. addition, study also compares imaging results different neural networks, show that DesUNet network improves feature information storage ability enhances reconstruction. It not only clearly restores original appearance object, but extracts physical about object. order further verify power conducted indoor near distance outdoor experiments. For reconstructed objects, could be identified. confidence level reach above 0.9 YOLO. The good robustness generalization.

Language: Английский

Citations

0

Instant-SFH: Non-Iterative Sparse Fourier Holograms Using Perlin Noise DOI Creative Commons
David Li, Susmija Jabbireddy, Yang Zhang

et al.

Sensors, Journal Year: 2024, Volume and Issue: 24(22), P. 7358 - 7358

Published: Nov. 18, 2024

Holographic displays are an upcoming technology for AR and VR applications, with the ability to show 3D content accurate depth cues, including accommodation motion parallax. Recent research reveals that only a fraction of holographic pixels needed display images high fidelity, improving energy efficiency in future displays. However, existing iterative method computing sparse amplitude phase layouts does not run real time; instead, it takes hundreds milliseconds render image into hologram. In this paper, we present non-iterative computation Fourier holograms uses Perlin noise image-plane phase. We conduct simulated optical experiments. Compared Gaussian-weighted Gerchberg-Saxton method, our achieves time improvement over 600 times while producing nearly equal PSNR SSIM quality. The real-time performance enables presentation dynamic crucial such as video streaming interactive visualization, on

Language: Английский

Citations

0